Human eosinophil-lymphocyte interactions

Weller, Peter F.; Lim, Kaiser G.

doi:10.1590/s0074-02761997000800023

Cited by 12 publications

(9 citation statements)

References 104 publications

(60 reference statements)

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“…Mast cells and basophils are both able to produce histamine and cytokines, like IL-4, IL-10 and IL-13 (Falcone et al 2000, Mazzoni et al 2001, which could act on immature dendritic cells. Eosinophils have also been described as cytokine producing APC cells (Weller & Lim 1997, Shi et al 2000. Platelets, abundantly present in the blood lake, could be another player.…”

Section: The Host Responsementioning

confidence: 99%

Leishmanial infection: analysis of its first steps. A review

Almeida

Vilhena²,

Barral

et al. 2003

Mem. Inst. Oswaldo Cruz

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Section: The Host Responsementioning

confidence: 99%

Leishmanial infection: analysis of its first steps. A review

Almeida

Vilhena²,

Barral

et al. 2003

Mem. Inst. Oswaldo Cruz

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“…To evaluate the specificity of the adhesive priming effect, we tested the ability of anti-ICAM-3 mAb to block stimulated eosinophil secretion. Adhesion-mediated augmentation of activated LTC 4 CD4 ϩ T cell secretion of cytokines caused by activation or adhesion did not cause the augmented secretion of LTC 4 from eosinophils. Both GM-CSF and IL-5 were below threshold levels of detection in triplicate samples from three separate eosinophil donors and below that necessary to augment stimulated eosinophil secretion in vivo (19).…”

Section: Adhesion Of Eosinophils To Cd4mentioning

confidence: 79%

“…ϩ T cells results in significant priming of LTC 4 secretion by FMLP LTC 4 secretion cause by FMLP plus cytochalasin B was significantly augmented to 2022 Ϯ 276.8 pg/ml when the eosinophils were adherent to CD4 ϩ T cells vs 1455 Ϯ 259.3 pg/ml for nonadherent, activated eosinophils ( p Ͻ 0.05; Fig. 6).…”

Section: Adhesion Of Eosinophils To Cd4mentioning

confidence: 93%

“…Many of these cytokines also have direct effects on eosinophil maturation, migration, and survival (1)(2)(3). While Th2 cell-specific cytokines alone have no effect on eosinophil secretion of bronchoactive eicasonoids, they substantially augment, or prime, eosinophil secretion in the presence of other activators (4).…”

Section: D4mentioning

confidence: 94%

“…At the end of adhesion, 10 Ϫ6 M FMLP (Sigma-Aldrich) and 5 g/ml cytochalasin B (Sigma-Aldrich) were added to wells to activate all eosinophils. We have previously demonstrated that this results in maximal secretion of LTC 4 and granular proteins of eosinophils (17). Activation was terminated after 30 min by plate centrifugation with low centrifugal force (500 rpm) for 5 min at 4°C.…”

Section: Ltc 4 Assaymentioning

confidence: 99%

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CD4+ T Cell and Eosinophil Adhesion Is Mediated by Specific ICAM-3 Ligation and Results in Eosinophil Activation

Douglas

Leff

Sperling

2000

The Journal of Immunology

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T cells and eosinophils, which are found in close proximity in asthmatic lungs, express many surface receptors that are counterligands. These data suggest that direct interactions between these cell types could play an important role in regulating airway inflammation in asthma. We examined the effect of selective adhesion between counterligands on human eosinophils and CD4+ T cells to determine 1) the existence of specific adhesive interactions and 2) if augmented specific adhesion to CD4+ T cells also caused augmented secretion of leukotriene C4 (LTC4) from eosinophils. A new method for binding of human CD4+ T cells to microwell plates was developed, which allowed for specific quantitative assessment of eosinophil adhesion to individual CD4+ T cells in culture. Adhesion of CD4+ T cells to eosinophils was minimal in unstimulated cells but increased after activation of T cells by PMA. Augmented adhesion was regulated substantially through binding of ICAM-3 and only minimally by ICAM-1. We further evaluated whether this specific adhesion up-regulated stimulated secretion of LTC4 from eosinophils. Adhesion with CD4+ T cells augmented eosinophil secretion of LTC4 caused by FMLP plus cytochalasin. Blockade of ICAM-3, as well as ICAM-1, inhibited completely the augmented secretion of eosinophil LTC4. We demonstrate that eosinophils and CD4+ T cells are capable of ligand-specific adhesion that is mediated predominantly by ICAM-3 ligation and that this binding causes augmented eosinophil secretion.

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Eosinophil cationic protein- and eosinophil-derived neurotoxin/eosinophil protein X-immunoreactive eosinophils in prurigo nodularis

Johansson

Liang

Marcusson

et al. 2000

Archives of Dermatological Research

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It is known that eosinophils are actively involved in allergy and inflammation. The granular components of eosinophils, eosinophil cationic protein (ECP) and eosinophil-derived neurotoxin/eosinophil protein X (EDN/EPX), play an important role in such allergic and inflammatory processes. Prurigo nodularis is a chronic inflammatory skin disease with obvious cutaneous nervous involvement. To detect ECP and EDN/ EPX expression in the eosinophils and their relation to nerve fibres in prurigo nodularis, ECP and EDN/EPX single-labelling immunofluorescence, and ECP and PGP 9.5 double-labelling immunofluorescence, were performed. In prurigo nodularis lesional skin, the ECP- and EDN/EPX-containing cells, which were mainly distributed in the upper dermis, were significantly increased in number compared to their numbers in uninvolved and normal skin. The immunoreactivity of ECP and EDN/EPX in prurigo lesional skin was stronger than in uninvolved skin or control skin. The PGP 9.5-immunoreactive nerves were also increased in number in the areas where there were increased eosinophils. The nerves were in close proximity to eosinophils, and occasionally even seemed to be in contact. The present results indicate that the cutaneous nerves and the ECP- and EDN/EPX-containing eosinophils are possibly involved in the pathogenesis of the disease. The close relationship of nerves and eosinophils indicates that the cutaneous nerves may influence eosinophil function in the chronic inflammatory states of prurigo nodularis. ECP and EDN/EPX could thus be released to the local tissue and modulate the inflammation of the prurigo nodularis lesion.

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Human eosinophil-lymphocyte interactions

Cited by 12 publications

References 104 publications

Leishmanial infection: analysis of its first steps. A review

Leishmanial infection: analysis of its first steps. A review

CD4+ T Cell and Eosinophil Adhesion Is Mediated by Specific ICAM-3 Ligation and Results in Eosinophil Activation

Eosinophil cationic protein- and eosinophil-derived neurotoxin/eosinophil protein X-immunoreactive eosinophils in prurigo nodularis

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