Modulation by IL-10 of antigen-induced allergic responses in mice

Zuany-Amorim, Cláudia; Vargaftig, B.B.; Pretolani, Marina

doi:10.1590/s0074-02761997000800019

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…After three washes in RPMI (Life Technologies, Paisley, U.K.), 5 ϫ 10 5 splenocytes/250 l RPMI supplemented with 10% FBS (Life Technologies), 2 mM L-glutamine (Life Technologies), 100 g/ml streptomycin (Life Technologies), and 100 U/ml penicillin (Life Technologies) were added to each well in triplicate and then incubated for 72 h at 37°C in 5% CO 2 in the presence or the absence of 80 g/ml OVA. For anti-CD3 activation cells were cultured in 96-multiwell plates coated with 4.5 g/ml immobilized anti-CD3 mAb 145-2C11 (BD PharMingen, Oxford, U.K.) for 48 h (19). Plates were then centrifuged (80 ϫ g, 5 min, 4°C), and IL-4 and IFN-␥ levels in the supernatants were determined by ELISA (R&D Systems, Oxon, U.K.).…”

Section: Splenocyte Activationmentioning

confidence: 99%

Long-Term Protective and Antigen-Specific Effect of Heat-Killed Mycobacterium vaccae in a Murine Model of Allergic Pulmonary Inflammation

Zuany-Amorim

Manlius

Trifilieff

et al. 2002

The Journal of Immunology

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106

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This report examines the effect of heat-killed Mycobacterium vaccae in a mouse model of allergic pulmonary inflammation. The s.c. administration of M. vaccae 3 wk before the immunization significantly reduced Ag-induced airway hyperreactivity and the increase in the numbers of eosinophils observed in the bronchoalveolar lavage fluid, blood, and bone marrow, even though no detectable changes in either cytokine (IL-4, IL-13, IL-5, and IFN-γ) or total IgE levels were observed. Furthermore, transfer of splenocytes from OVA-immunized and M. vaccae-treated mice into recipient, OVA-immunized mice significantly reduced the allergen-induced eosinophilia by an IFN-γ-independent mechanism, clearly indicating that the mechanism by which M. vaccae induces its inhibitory effect is not due to a redirection from a predominantly Th2 to a Th1-dominated immune response. The protective effect of M. vaccae on the allergen-induced eosinophilia lasted for at least 12 wk after its administration, and the treatment was also effective in presensitized mice. Moreover, the allergen specificity of the inhibitory effect could be demonstrated using a double-immunization protocol, where M. vaccae treatment before OVA immunization had no effect on the eosinophilic inflammation induced by later immunization and challenge with cockroach extract Ag. Taken together, these results clearly demonstrate that M. vaccae is effective in blocking allergic inflammation by a mechanism independent of IFN-γ, induces long term and Ag-specific protection, and therefore has both prophylactic and therapeutic potential for the treatment of allergic diseases.

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Section: Splenocyte Activationmentioning

confidence: 99%

Long-Term Protective and Antigen-Specific Effect of Heat-Killed Mycobacterium vaccae in a Murine Model of Allergic Pulmonary Inflammation

Zuany-Amorim

Manlius

Trifilieff

et al. 2002

The Journal of Immunology

Self Cite

106

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“…challenge with a small dose of the antigen results in accumulation of leucocytes in the peritoneum. 16,17 Eosinophils are prominently involved in this inflammatory reaction, which also produce significant bone marrow eosinophilia involving T-cell-derived cytokines such as IL-5, IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF). 18,19 Carrageenan-induced oedema in the paw is a widely used model to investigate the physiopathology of an acute local inflammation.…”

Section: Introductionmentioning

confidence: 99%

Cell‐mediated immune response to unrelated proteins and unspecific inflammation blocked by orally tolerated proteins

Ramos¹,

Rodrigues²,

Azevedo³

et al. 2009

Immunology

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Oral tolerance promotes a generalized decrease in specific immune responsiveness to proteins previously encountered via the oral route. In addition, parenteral immunization with a tolerated protein also triggers a significant reduction in the primary responsiveness to a second unrelated antigen. This is generally explained by 'innocent bystander suppression', suggesting that the transient and episodic effects of inhibitory cytokines released by contact with the tolerated antigen would block responses to the second antigen. In disagreement with this view, we have previously shown that: (i) these inhibitory effects do not require concomitance or contiguity of the injections of the two proteins; (ii) that intravenous or intragastric exposures to the tolerated antigen are not inhibitory; and (iii) that the inhibitory effect, once triggered, persists in the absence of further contact with the tolerated protein, possibly by inhibition of secondary responsiveness (immunological memory). The present work confirms that immunological memory of the second unrelated antigen is hindered by exposure to the tolerated antigen and, in addition, shows that this exposure: (i) inhibits the inflammation triggered by an unrelated antigen through the double effect of inhibiting production of leucocytes in the bone marrow and blocking their migration to inflammed sites; and (ii) significantly blocks footpaw swelling triggered by carrageenan. Taken together, these results conclusively demonstrate that inhibitory effects of parenteral injection of tolerated antigens are much more general than suggested by the 'innocent bystander suppression' hypothesis.

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The Effects of Il-6 and Il-10 and Their Specific Antibodies in the Acute Inflammatory Responses Induced by Carrageenan in the Mouse Model of Pleurisy

2002

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Modulation by IL-10 of antigen-induced allergic responses in mice

Cited by 3 publications

References 35 publications

Long-Term Protective and Antigen-Specific Effect of Heat-Killed Mycobacterium vaccae in a Murine Model of Allergic Pulmonary Inflammation

Long-Term Protective and Antigen-Specific Effect of Heat-Killed Mycobacterium vaccae in a Murine Model of Allergic Pulmonary Inflammation

Cell‐mediated immune response to unrelated proteins and unspecific inflammation blocked by orally tolerated proteins

The Effects of Il-6 and Il-10 and Their Specific Antibodies in the Acute Inflammatory Responses Induced by Carrageenan in the Mouse Model of Pleurisy

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