Differential Serodiagnosis of Human Infections Caused by Trypanosoma cruzi and Leishmania spp. Using ELISA with a Recombinant Antigen (rTc24)

Passos, Valéria Maria Azeredo; Volpini, Ângela; Braga, Érika Martins; Lacerda, Patricia; Ouaissi, Ali; Lima‐Martins, Maria Virginia C.; Krettli, Antoniana U.

doi:10.1590/s0074-02761997000600011

Cited by 12 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The function of this protein remains largely unknown, but it has been hypothesized that it participates in an acyl-calcium switch system (12), and this proposal is supported by experimental data showing a Ca 2ϩ -dependent conformational change (26). Since it was cloned for the first time, its utility in the diagnosis of T. cruzi infection has been well established (14,18,25,(33)(34)(35), including the possibility of using its gene sequence for PCR diagnosis (31). The rapid reduction of antibodies against calflagin in patients that respond to trypanocidal treatment has also been demonstrated, indicating that calflagin may be used in therapy follow-up (33).…”

Section: Discussionmentioning

confidence: 99%

“…The rapid reduction of antibodies against calflagin in patients that respond to trypanocidal treatment has also been demonstrated, indicating that calflagin may be used in therapy follow-up (33). It is worth emphasizing that several isoforms of calflagin were tested, by a number of research groups (14,18,25,34,35), against panels of sera, including samples from Leishmania spp.-infected patients. In all these studies, the authors detected low levels of cross-reactivity for the sera from patients infected by Leishmania.…”

Section: Discussionmentioning

confidence: 99%

“…However, Passos et al reported that the reactivity of patient sera against this protein in a geographic area that was coendemic for T. cruzi and Leishmania spp. infections were significantly higher than the prevalence of chagasic infection (25). These authors determined the reactivity percentage of 335 sera from patients presenting clinical manifestations of cutaneous and mucocutaneous leishmaniasis.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, no Leishmania orthologs have been described up to now. Several studies on the diagnostic profile of this recombinant protein identified its use as a valuable antigen for the serological detection of the infection in the indeterminate phase (14,18,25,34,35) and for therapy evolution follow-up (33). Several works also established that sera from Leishmania spp.-infected individuals have low levels of cross-reactivity with T. cruzi calflagins (14,34,35).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Use of Full-Length Recombinant Calflagin and Its C Fragment for Improvement of Diagnosis ofTrypanosoma cruziInfection

et al. 2005

View full text Add to dashboard Cite

Serological diagnosis of Trypanosoma cruzi infection is hampered by issues related to test specificity due to the cross-reactivity of most antigens with proteins of related parasites such as Leishmania spp. The recombinant calflagins are considered relevant antigens for the diagnosis of infection by Trypanosoma cruzi. In the present work, we describe two genes coding for putative calflagins in Leishmania major with the N-terminal moieties presenting high similarity with T. cruzi genes. This fact raised questions about their role in some cross-recognition of this antigen by sera from Leishmania spp.-infected individuals. The complete T. cruzi calflagin and two fragments of the protein, consisting of 146 amino acids of the N-terminal and 65 amino acids of the C-terminal regions, were expressed and evaluated against a panel of sera, which included wellcharacterized samples from T. cruzi, and Leishmania-infected patients. We were able to show that sera from Leishmania (Viannia) braziliensis-infected individuals recognized the recombinant full-length calflagin. Both the N-terminal and the complete protein presented the same high sensitivity (98.5% of sera from T. cruziinfected patients was detected) but different specificities (94% and 98%, respectively, when evaluated against sera from people not infected by T. cruzi, including 15 sera from people infected with L. braziliensis). The C-terminal fragment presented low sensitivity (70%) but 100% specificity. We propose the use of these antigens in two sequential assays to optimize the serological diagnosis of T. cruzi infection in humans in geographic areas where Leishmania spp. infection is coendemic.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Use of Full-Length Recombinant Calflagin and Its C Fragment for Improvement of Diagnosis ofTrypanosoma cruziInfection

et al. 2005

View full text Add to dashboard Cite

show abstract

“…In addition, this antigenic heterogeneity does not allow the differential diagnosis between the acute and chronic phases and also among the clinical manifestations (48). Indeed, discrepancies or inconclusive results, such as false-positive reactions caused by cross-reactivity with antibodies induced by other pathogens (mainly Leishmania) and false-negatives, may occur (35,41,42). For these reasons, the World Health Organization recommends using at least two of those serologic tests in parallel (52).…”

mentioning

confidence: 99%

Trypanosoma cruziRecombinant Complement Regulatory Protein: a Novel Antigen for Use in an Enzyme-Linked Immunosorbent Assay for Diagnosis of Chagas' Disease

Meira¹,

Galvão²,

Gontijo

et al. 2002

J Clin Microbiol

View full text Add to dashboard Cite

Currently, diagnosis of Chagas' disease is based on serological methods, but due to the high occurrence of inconclusive results, more reliable methods are needed. The use of recombinant antigens for serodiagnosis of Chagas' disease is recommended in order to increase the sensitivity and specificity of the serological tests. The Trypanosoma cruzi complement regulatory protein (CRP) is a surface glycoprotein present on the trypomastigote forms of the parasite, and the recombinant CRP (rCRP) was cloned in a mammalian expression system and purified by affinity chromatography. The purified recombinant protein was used as an antigen in an enzyme-linked immunosorbent assay (rCRP ELISA) in order to verify its sensitivity and specificity compared with other established methods. In this evaluation, a panel of 184 serum samples distributed among chronic chagasic patients (n ‫؍‬ 65), blood bank donors (n ‫؍‬ 100), and patients infected with Leishmania spp. (n ‫؍‬ 19) was used. The sensitivity and specificity of the rCRP ELISA were 100% when compared to conventional serology and complement-mediated lysis tests from these groups. When hemoculture and PCR tests were evaluated for diagnosis of chronic chagasic patients, using the rCRP ELISA as a reference test, the positivities were found to be 64.62 and 81.54%, respectively, showing a higher degree of sensitivity of the test. The data demonstrate that rCRP ELISA was able to discriminate between chronic chagasic patients and nonchagasic individuals, such as blood donors and patients with leishmaniasis. Thus, the rCRP is an excellent antigen for use in Chagas' disease diagnosis, due to the absence of false-negative or false-positive results.

show abstract

Validation of a Leishmania infantum ELISA rapid test for serological diagnosis of Leishmania chagasi in dogs

Marcondes

Biondo

Gomes

et al. 2011

Veterinary Parasitology

View full text Add to dashboard Cite

Differential Serodiagnosis of Human Infections Caused by Trypanosoma cruzi and Leishmania spp. Using ELISA with a Recombinant Antigen (rTc24)

Cited by 12 publications

References 0 publications

Use of Full-Length Recombinant Calflagin and Its C Fragment for Improvement of Diagnosis ofTrypanosoma cruziInfection

Use of Full-Length Recombinant Calflagin and Its C Fragment for Improvement of Diagnosis ofTrypanosoma cruziInfection

Trypanosoma cruziRecombinant Complement Regulatory Protein: a Novel Antigen for Use in an Enzyme-Linked Immunosorbent Assay for Diagnosis of Chagas' Disease

Validation of a Leishmania infantum ELISA rapid test for serological diagnosis of Leishmania chagasi in dogs

Contact Info

Product

Resources

About