Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae)

Bello, Felio J.; Brochero, Helena; Bóshell, Jorge; Olano, Víctor Alberto; Rey, Gloria

doi:10.1590/s0074-02761997000100027

Cited by 8 publications

(13 citation statements)

References 21 publications

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“…In the present study, an optimal incubation time of between 16-20 h was determined to achieve successful cell cultures, which corresponds to approximately 2/3 of the total time of embryo formation before the egg hatches. A similar finding has been published in previous studies on the establishment of cell lines derived from mosquitoes (Pant & Dandha 1980, Bello et al 1997. In addition to the above-mentioned incubation period, the embryonated eggs that produced the best results at the initiation of the cell cultures were those that received an additional 4-6 h of incubation at 28ºC.…”

Section: Discussionsupporting

confidence: 85%

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Segura

Santamaría

Cabrera

et al. 2012

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 85%

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Segura

Santamaría

Cabrera

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…Si bien, esta característica de crecimiento lento de las células, en la etapa de iniciación de los cultivos, ha sido común al utilizar otras especies de mosquitos (14,16,17,45,46), probablemente, también contribuyó a la interferencia del crecimiento celular la adición de albendazol al medio de cultivo, usado durante un tiempo prolongado. Esta droga fue empleada porque previamente había demostrado actividad contra microsporidios, tanto en ensayos in vitro de infecciones inducidas (47)(48)(49), como en infecciones naturales de embriones utilizados en explantes para obtener cultivos celulares (16). Sin embargo, después de descartar contaminación de los cultivos con parásitos, el fármaco disuelto en dimetilsulfóxido (DMSO) fue suspendido.…”

Section: Discussionunclassified

“…aegypti; posteriormente, se establecieron otras líneas celulares de esta misma especie obtenidas, en orden cronológico, por Singh en 1967 (10), Peleg en 1968 (11,12) (14)(15)(16)(17).…”

unclassified

Características de nuevos cultivos celulares derivados de tejidos embrionarios de Aedes aegypti (Diptera: Culicidae).

Silva¹,

Escovar²,

Bello³

2005

biomedica

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Introducción. Los cultivos celulares de insectos son una metodología útil en estudios biomédicos y tecnológicos. Objetivo. El propósito principal del presente trabajo fue obtener y caracterizar cultivos celulares derivados de tejidos embrionarios de Aedes aegypti. Materiales y métodos. Se emplearon huevos embrionados para los explantes de tejidos en los medios de cultivos MM/VP12 y L-15/Grace, con suplemento de 20% de suero fetal bovino y una mezcla al 1% de antibiótico y antimicótico, con un rango de pH entre 6,8 y 7,0. Los cultivos se incubaron a una temperatura de 28 o C sin atmósfera de CO 2 . Resultados. El crecimiento celular se obtuvo en el medio L-15/Grace, 3 semanas después de haber sido sembrados los tejidos embrionarios; sin embargo, se necesitaron 6 meses para la formación de la monocapa confluente. Desde agosto de 2003 hasta junio de 2004, se habían realizado 28 subcultivos. Las células se caracterizaron morfológicamente; predominaron las formas epitelioides en subcultivos de pases altos. También se reconocieron las particularidades morfométricas del cariotipo y, además, se determinaron los perfiles isoenzimáticos y moleculares de los cultivos celulares, los cuales se compararon con muestras de adultos de la especie tomadas de la misma colonia y con líneas celulares derivadas de otros insectos. Discusión. Estas células representan, potencialmente, un importante sistema in vitro en investigaciones básicas y aplicadas. Palabras clave: Aedes aegypti, cultivos celulares, vesículas, cariotipos, isoenzimas, RAPD-PCR.Characteristics of new cell cultures derived from embryonic tissues of Aedes aegypti (Diptera: Culicidae) Introduction. Cell cultures from insects are a useful methodology in technological and biomedical studies.Objective. The present work was aimed at obtaining and characterizing cell cultures derived from Aedes aegypti embryonic tissues. Materials and methods. Embryonated eggs were used for embryonic tissue explants in L-15/ Grace and MM/VP12 culture media, supplemented with 20% fetal bovine serum and a mixture of 1% antimycotic and antibiotics, at a pH ranging from 6.8 to 7.0. The incubation temperature was 28 o C; a CO 2 atmosphere was not required. Results. Cell growth was obtained in L-15/Grace medium three weeks after embryonic tissues explants. Six months were required for achieving a confluent monolayer. Twenty-eight serial cell subcultures were carried out from August 2003 to June 2004. Cell morphology was characterized as epithelial in the later subcultures. Karyotype morphometry as well as molecular and isozymatic profiles were established. The cultures were compared with adult samples from the species taken from the same colony and with cell lines derived from other insects.

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“…Cell samples were simultaneously run with adult and pupae extracts originating from the same colony. The current cell line's isozymatic patterns were compared to the Anopheles albimanus cell line LSB-AA695BB (Bello et al 1997). The only band or the band having the greatest migration distance from An.…”

Section: Methodsmentioning

confidence: 99%

“…taeniorhynchus (Bello et al 1995), An. albimanus SB-AA695BB (Bello et al 1997) and Lutzomyia longipalpis (Tesh 1983, Rey & Ferro Bello 2000 cell lines.…”

Section: A B C Dmentioning

confidence: 99%

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

Bello

Rodrı́guez

Escovar

et al. 2001

Mem. Inst. Oswaldo Cruz

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Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae)

Abstract: A new cell line designated LSB-AA695BB, was established from embryos of the mosquito Anopheles albimanus. The primary culture was initiated in April, 1995, and

Cited by 8 publications

References 21 publications

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)

Características de nuevos cultivos celulares derivados de tejidos embrionarios de Aedes aegypti (Diptera: Culicidae).

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

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