Comparison of Two Enzyme Immunoassays for the Detection of Antibody to Hepatitis B Virus Core Antigen

Souto, Francisco José Dutra; Fontes, Cor Jésus Fernandes; Oliveira, Jaqueline Mendes de; Gaspar, Ana; Lyra, Luiz Guilherme Costa

doi:10.1590/s0074-02761997000100018

Cited by 6 publications

(5 citation statements)

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“…Anti‐HBs testing was not implemented as it is less sensitive in detecting previous HBV contact (Souto et al , 1997). In addition, anti‐HBs could be absent during the convalescence period or in patients who resolved infection but lost detectable anti‐HBs (Dienstag & Ryan, 1982; Behzad‐Behbahani et al , 2006).…”

Section: Discussionmentioning

confidence: 99%

Significance of detecting anti-HBc among Egyptian male blood donors negative for HBsAg*

Antar

El-Shokry

Hamid

et al. 2010

Transfusion Medicine

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Section: Discussionmentioning

confidence: 99%

Significance of detecting anti-HBc among Egyptian male blood donors negative for HBsAg*

Antar

El-Shokry

Hamid

et al. 2010

Transfusion Medicine

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“…In the present study, anti‐HBs testing was not implemented as it is less sensitive in detecting previous HBV contact (Souto et al , 1997). In addition, anti‐HBs could be absent during convalescence period or in patients who resolved infection but lost detectable anti‐HBs (Dienstag & Ryan, 1982; Behzad‐Behbahani et al , 2006).…”

Section: Discussionmentioning

confidence: 99%

Anti‐HBc screening in Egyptian blood donors reduces the risk of hepatitis B virus transmission

El-Zayadi

Ibrahim

Badran

et al. 2008

Transfusion Medicine

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Occult hepatitis B virus (HBV) in blood donors is considered as a potential risk for transmission of HBV infection. The aim of this study was to determine the prevalence of anti-hepatitis B core antibody (anti-HBC) positivity in Egyptian blood donations as well as to estimate the frequency of HBV-DNA in anti-HBc-positive donations. The study included 760 Egyptian healthy blood donors, representing 26 different Egyptian governorates screened according to routine practice for the presence of hepatitis B surface antigen (HBsAg), hepatitis C virus (HCV) antibodies (Abs), HIV-1/2 Abs and Treponema Abs. The accepted blood units for donation were tested for the presence of total anti-HBc Abs by two tests. Positive units for anti-HBc were further tested for HBV-DNA by polymerase chain reaction. According to routine screening, a total of 48/760 units (6.3%) were rejected [38 (5%) HCV-Ab-positive units, 9 (1.18%) HbsAg-positive units and 1 (0.13%) Treponema-Ab-positive unit]. Among the accepted blood units for donation, prevalence of anti-HBc was 78/712 units (10.96%). HBV-DNA was detected in 9/78 (11.54%) of the anti-HBc-positive units, and thus, occult HBV infection was detected in 9/712 (1.26%) of the accepted blood donations. Implementing anti-HBc test to the routine assay for the forthcoming two decades would certainly eliminate possible HBV-infected units. Rejection of these units will be beneficial to decrease the risk of HBV transmission with its potential consequences particularly in immunocompromised recipients.

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“…The samples were analyzed for HBV surface antigen and antibody (HBsAg and anti-HBs, respectively), total and IgM antibodies against HBV core antigen (anti-HBc and IgM anti-HBc, respectively), and IgM antibody against hepatitis A virus (IgM anti-HAV), using enzyme immunoassays (EIA) prepared by Instituto Oswaldo Cruz (FIOCRUZ). The reagent kits for the HB-sAg and total anti-HBc EIA detection were recently evaluated in a blind comparison with a similar EIA kit as a gold standard showing high agreement rates [Souto et al, 1997]. The performance of the FIOCRUZ IgM anti-HBc test is periodically checked by comparison with similar commercial kits at the Department of Virology, Instituto Oswaldo Cruz.…”

Section: Methodsmentioning

confidence: 99%

Outbreak of hepatitis B virus in recent arrivals to the Brazilian Amazon

1998

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An outbreak of acute hepatitis cases in a small community took place 6 months after the community's arrival to the Brazilian Amazon. An epidemiological investigation was performed and included residents aged more than two years. Study subjects were interviewed and bled to test for hepatitis markers by enzyme immunoassays. Around 80% of the village population was surveyed. The overall prevalence of hepatitis B virus (HBV) markers was 75.1% (281/374). The surface antigen of HBV (HBsAg) and the IgM class antibody against hepatitis B core antigen (IgM anti-HBc) were present in 10.4% and 9.6%, respectively. Evidence of HBV-HDV (Delta virus) coinfection or hepatitis C infection was not found. IgM class antibody against hepatitis A virus was uncommon (3.7%). Follow-up evaluation 6 and 12 months later were carried out to identify new HBV infections. An incidence rate of 7.2 new infections per 100 exposed subjects per month was found. Average individual risk for HBV infection among susceptible inhabitants of the village between June 1995 and June 1996 can be estimated at 57.6%. The predominant HB-sAg subtype found (ayw3) suggests that immigrants may have carried HBV from the original area. Time living in the study region was significantly associated with HBV markers in analysis for linear trend and logistic regression analysis. Environmentally related factors may have facilitated HBV transmission.

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Comparison of Two Enzyme Immunoassays for the Detection of Antibody to Hepatitis B Virus Core Antigen

Cited by 6 publications

References 0 publications

Significance of detecting anti-HBc among Egyptian male blood donors negative for HBsAg*

Significance of detecting anti-HBc among Egyptian male blood donors negative for HBsAg*

Anti‐HBc screening in Egyptian blood donors reduces the risk of hepatitis B virus transmission

Outbreak of hepatitis B virus in recent arrivals to the Brazilian Amazon

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