Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory

Campos, Rozalia Mendes Figueira; Andrade, Sônia Gumes

doi:10.1590/s0074-02761996000600029

Cited by 10 publications

(19 citation statements)

References 22 publications

(20 reference statements)

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“…Por el contrario, otros autores han encontrado que las infecciones con T. cruzi II son bastante graves y que comprometen algunos tejidos como recto y esófago (1,16,31). Los resultados encontrados en este estudio muestran que la parasitemia y el tropismo por órganos de parásitos pertenecientes al grupo T. cruzi II es menor que la de parásitos del grupo T. cruzi I, con tropismo preferencial por bazo e hígado.…”

Section: Cuadrounclassified

Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

Botero¹,

Mejía²,

Triana³

2007

biomedica

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Introducción. T. cruzi I y T. cruzi II son grupos genéticamente diferentes y se cree que dicha variabilidad es determinante del tropismo tisular en el hospedero vertebrado y responsable de las diversas manifestaciones clínicas de la enfermedad de Chagas. Objetivo. Caracterizar biológica y genéticamente dos clones colombianos de los grupos T. cruzi I y II en el modelo murino. Materiales y métodos. Las cepas CAS15 y AF1 pertenecientes a los grupos T. cruzi I y II fueron clonadas en medio semisólido. Un clon de cada una de ellas y una mezcla de ambos se utilizaron para infectar ratones, los cuales se sacrificaron a diferentes tiempos post-infección. Para analizar la presencia del parásito en sangre y diferentes órganos, se utilizaron el microhematocrito y la reacción en cadena de la polimerasa con los marcadores de la secuencia satélite del ADN nuclear y con el espaciador intergénico del gen mini-exón. Resultados. El clon T. cruzi I fue más infectivo, observándose un tropismo preferencial por corazón, recto y músculo esquelético, mientras que el clon T. cruzi II presentó un tropismo preferencial por bazo e hígado. Durante la infección con la mezcla de los clones, se observó que el clon T. cruzi I predominó sobre el T. cruzi II tanto en sangre como en órganos. Conclusiones. Los resultados confirman que las diferencias genéticas entre los grupos de T. cruzi podrían estar determinando el tropismo tisular y de esta manera jugar un papel fundamental en el entendimiento de las manifestaciones clínicas de la enfermedad de Chagas en Colombia.Palabras claves: Trypanosoma cruzi, enfermedad de Chagas, variación (Genética), tropismo. Biological and genetic characterization of two Colombian clones of Trypanosoma cruzi groups I and IIIntroduction. Genetic differences between T. cruzi I and T. cruzi II may determine differences in their tissue tropism in the vertebrate host and may also be responsible for the differences in clinical manifestations of Chagas disease. Objective. Two Colombian clones of the T. cruzi groups I and II were characterized biologically and genetically in a murine model. Materials and methods. Strains Cas15 and AF1 belonging to the T. cruzi groups I and II were cloned in semisolid medium. A clone of each strain and a mix of both were used to infect mice; the mice were subsequently sacrificed at selected post-infection intervals. In order to identify the parasite presence in blood and organs, two methods were used (a) microhematocrit and (b) polymerase chain reaction with primers for satellite DNA and the intergenic region of miniexon. Results. The T. cruzi I clone was more infectious, with a preferential tropism observed in heart, rectum and skeletal muscle, whereas clone T. cruzi II exhibited a preferential tropism for spleen and liver. During the infection with the clone mixture a predominance of the T. cruzi I clone over clone II in blood as well as in organs was observed. Conclusion. The results corroborate that the genetic differences between the T. cruzi groups correlate with their tissue tropism, an...

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Section: Cuadrounclassified

Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

Botero¹,

Mejía²,

Triana³

2007

biomedica

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“…From each positive animal, thus representing one clone, inoculum was individually obtained and injected into suckling mice. Five clones and 14 subclones have been obtained and submitted to biological, biochemical and genetic characterization (Campos & Andrade 1996, Campos et al 1999. Biological and biochemical characterization have shown that all the clones and subclones revealed parasitemic profils characteristics for Type II strains, and similar to the parental strain (21SF), in the infected mice (Campos & Andrade 1996).…”

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confidence: 99%

Trypanosoma cruzi: clonal structure of parasite strains and the importance of principal clones

Andrade

1999

Mem. Inst. Oswaldo Cruz

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“…Biological and isoenzymic characters of the isolated clones reproduced the patterns of the parental strain 7 . Molecular characterization of the clones isolated from the 21SF strain has been performed by the restriction fragment length polymorphism (RFLP) of the kDNA 8 .…”

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confidence: 59%

“…Investigations into the clonal constitution of the 21SF strain (biodeme Type II), in previous studies by Campos & Andrade 7 , have shown a homology between the isolated clones. It has 1: 320 to 1: 640 1: 80 to 1: 640 * Treated with benznidazole and untreated controls 21SF -P = 21SF parental strain been demonstrated that the profiles for the isoenzymes 5 phosphoglucomutase (PGM), glucose phosphate isomerase (GPI), alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT), expressed the zymodeme Z2 11 , that corresponds to the biodeme Type II, suggesting the predominance of a principal clone in this strain.…”

Section: Discussionmentioning

confidence: 88%

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Response to chemotherapy with benznidazole of clones isolated from the 21SF strain of Trypanosoma cruzi (biodeme Type II, Trypanosoma cruzi II)

Campos

Guerreiro

Sobral

et al. 2005

Rev. Soc. Bras. Med. Trop.

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Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory

Cited by 10 publications

References 22 publications

Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

Trypanosoma cruzi: clonal structure of parasite strains and the importance of principal clones

Response to chemotherapy with benznidazole of clones isolated from the 21SF strain of Trypanosoma cruzi (biodeme Type II, Trypanosoma cruzi II)

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