Utilization of a new culture medium in biochemical testes for the mycobacterial classification

Medeiros, Luciene; Callejas, Ana Milena; Valdivia, José A.

doi:10.1590/s0074-02761992000300017

Cited by 3 publications

(3 citation statements)

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“…All of them belong to the collection of the 'Instituto de Medicina Tropical Pedro Kourí (IPK), La Habana', Cuba. They were cultivated for 3 weeks at 37 uC in liquid UIT-L medium (a modified Löwenstein-Jensen medium) (Mederos et al, 1992), checked for purity, collected, washed with saline, killed at 120 uC for 20 min, and dried in a vacuum over P 2 O 5 .…”

Section: Methodsmentioning

confidence: 99%

Analysis of the structure of mycolic acids of Mycobacterium simiae reveals a particular composition of α-mycolates in strain ‘habana’ TMC 5135, considered as immunogenic in tuberculosis and leprosy

2007

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Section: Methodsmentioning

confidence: 99%

Analysis of the structure of mycolic acids of Mycobacterium simiae reveals a particular composition of α-mycolates in strain ‘habana’ TMC 5135, considered as immunogenic in tuberculosis and leprosy

2007

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“…All of them belonged to the collection of the IPK (Instituto de Medicina Tropical Pedro Kourí), La Habana, Cuba. They were cultivated in liquid UIT-L medium (Mederos et al, 1992) at 35 uC for 3 weeks, harvested, washed with saline, killed at 120 uC for 20 min and dried in a vacuum over P 2 O 5 (Mederos et al, 2007).…”

Section: Methodsmentioning

confidence: 99%

Structural studies of cord factors from Mycobacterium simiae related to the capacity for tumour necrosis factor alpha (α-TNF) induction

et al. 2010

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The structure of cord factor was studied in several strains of Mycobacterium simiae, including 'habana' TMC 5135, considered as highly immunogenic in experimental tuberculosis and leprosy. The mycolic acids liberated from cord factor were identified in all cases as a9-, a-and ketomycolates. According to the general NMR and MS data, a9-mycolates were mono-unsaturated and contained from 64 to 68 carbon atoms, whereas a-mycolates mainly presented two 2,3-disubstituted cyclopropane rings and a chain length of 80-91 carbon atoms; keto-mycolates mostly contained one cyclopropane ring and 85-91 carbon atoms. Taking into account the 1 H-NMR results, strains varied in the ratio of the different mycolates, and the high levels of ketomycolates found in the cord factors of TMC 5135 and ATCC 25275 T stood out. Notably, MS revealed that the odd carbon number series of a-mycolates (C87-C89) predominated in the cord factor of TMC 5135, in contrast to the remaining studied strains, in which the even (C84-C86) and odd carbon number series appeared more equal. The fine structural differences detected among the cord factors studied did not seem to be relevant to the general capacity of these molecules to induce the secretion of tumour necrosis factor alpha, as the cord factors from several strains of M. simiae (TMC 5135, IPK-342 and ATCC 25275 T ) induced similar amounts of this cytokine in RAW 264.7 cells.

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“…All of them belong to the culture collection of the Instituto Pedro Kourí (IPK) de Medicina Tropical, La Habana, Cuba. The strains were cultivated for 3 weeks at 37°C in liquid UIT‐L medium (a modified Löewentein‐Jensen medium) (Mederos et al. 1992), checked for purity, collected, washed in saline, killed at 120°C for 20 min and dried in a vacuum over P 2 O 5 .…”

Section: Methodsmentioning

confidence: 99%

New variants of polar glycopeptidolipids detected inMycobacterium simiae, includinghabanastrains, as evidenced by electrospray ionization-ion trap-mass spectrometry

Mederos

Valdivia

Valero-Guillén

2008

Journal of Applied Microbiology

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Aims: To determine the composition of polar glycopeptidolipids (pGPLs) of Mycobacterium simiae and, particularly, those of 'habana' strains, in a search for specific markers given the immunogenic potential of 'habana' TMC 5135 in experimental tuberculosis. Methods and Results: pGPLs were determined in free lipid extracts using electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS), working in both negative-and positive-ion mode. In the case of TMC 5135, the presence of the previously characterized GPL-II (containing 2,4-di-O-CH 3 glucuronic acid as distal sugar in the oligosaccharide antigenic moiety) and GPL-III (containing 4-O-CH 3 glucuronic acid as distal sugar) was confirmed using MS ⁄ MS and MS ⁄ MS ⁄ MS approaches. Interestingly, some 'habana' strains presented variants of GPL-II, designated GPL-II¢-A and GPL-II¢-B. A di-O-CH 3 -deoxyhexose (tentatively, 2,3-di-O-CH 3 -fucose) was identified as the penultimate sugar in the oligosaccharide moiety of GPL-II¢-A, whereas in GPL-II¢-B the penultimate sugar was fucose (tentative identification). On the contrary, the distal sugar of the oligosaccharide chain of pGPLs of Myco. simiae ATCC 25275T was identified as tri-O-CH 3 -glucuronic acid (designated GPL-sim T

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Utilization of a new culture medium in biochemical testes for the mycobacterial classification

Cited by 3 publications

References 0 publications

Analysis of the structure of mycolic acids of Mycobacterium simiae reveals a particular composition of α-mycolates in strain ‘habana’ TMC 5135, considered as immunogenic in tuberculosis and leprosy

Analysis of the structure of mycolic acids of Mycobacterium simiae reveals a particular composition of α-mycolates in strain ‘habana’ TMC 5135, considered as immunogenic in tuberculosis and leprosy

Structural studies of cord factors from Mycobacterium simiae related to the capacity for tumour necrosis factor alpha (α-TNF) induction

New variants of polar glycopeptidolipids detected inMycobacterium simiae, includinghabanastrains, as evidenced by electrospray ionization-ion trap-mass spectrometry

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