Identification and purification of a 72kDa antigen of Leishmania braziliensis braziliensis present on the surface and in the cytoplasm of the promastigotes and its specific recognition by sera from mucocutaneous leishmaniasis patients

Kutner, Shirley; Pellerin, Patrice; Brenière, Simone Frédérique; Desjeux, Philippe; Dédet, J. P.

doi:10.1590/s0074-02761988000500041

Cited by 5 publications

(3 citation statements)

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“…The 70.8 kDa protein showed reactivity with serum samples of patients with paracoccidioidomycosis and serum samples of patients with leishmaniasis, although at a lower frequency. In some analyses, the 72 kDa protein has been recognized as an important surface antigenic component, specific for L. braziliensis [21][22][23]. However, other researchers have identified the 70-72 kDa proteins as members of protein families from highly-preserved areas of DNA, and have also been recognized by sera of patients with visceral leishmaniasis, along with other illnesses, such as tuberculosis, toxoplasmosis and hydatidosis [24].…”

Section: Discussionmentioning

confidence: 99%

Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Szargiki¹,

Castro²,

Luz³

et al. 2009

Braz J Infect Dis

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Section: Discussionmentioning

confidence: 99%

Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Szargiki¹,

Castro²,

Luz³

et al. 2009

Braz J Infect Dis

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“…3(c)]. The prominent 60-kDa antigen may correspond to that described by Masuda et al (1989) as speci® c to ATL patients, and the prominent 72-kDa antigen may correspond with that described by Kutner et al (1988) as not cross-reacting with sera from cases of either visceral leishmaniasis or Chagas disease. Sequential, dual ELISA, with differently coloured detection products, although a useful adjunct to differential immunological screening of expression libraries (Miles and Clarke, 1989), failed to detect binding differences between sera from cases of LCL and MCL in the present study.…”

Section: Discussionmentioning

confidence: 82%

Immunological selection for Leishmania (Viannia) braziliensis antigens

Cuba¹,

Ogunkolade²,

Howard³

et al. 2001

Annals of Tropical Medicine And Parasitology

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Comparative ELISA and selective immunoblotting procedures were used in attempts to identify differential serological indicators of infection with the Leishmania (Viannia) braziliensis complex, infection with the L. braziliensis species, and therapeutic cure of localized or mucocutaneous leishmaniasis (LCL or MCL). Although mean ELISA absorbance values were significantly higher for MCL sera than for LCL sera, absorbance could not be used as a reliable indicator of the clinical form of disease. Immunoblotting profiles were similar with sera from MCL and LCL. Pre-adsorption with heterologous trypanosomatid antigens indicated that recognition of antigens of about 56, 60, 66, 72, 88 and 110 kDa might be specific to the subgenus Viannia. In two-colour, sequential, dual ELISA-based immunoblotting, no antigens recognized only by sera from MCL patients were detected. After glucantime therapy, immunoblotting profiles with LCL sera were reduced both in intensity and in the range of antigens detected; a 104-kDa antigen was newly detected with post-treatment LCL sera. Overall, the results show the value of differential immunological detection strategies and support the close relationship between species of the subgenus Viannia but fail to indicate a prognostic antigen for MCL.

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“…Few specific L. (V.) braziliensis antigens are described in the literature: a 72 kDa protease in the promastigote surface (Legrand et al 1987 ;Kutner et al 1988 ;Kutner, Pellerin & Brénière,1990) ; a 47-54 kDa glycoprotein (Rodríguez, Hernández & Merino, 1983 ;Misle, Márquez & Hernández, 1985 ;Nagakura et al 1986) ; a 25 kDa antigen by (Misle et al 1985) and 17 and 24 kDa promastigote stage-specific antigens (McMahon-Pratt, Bennett & Jaffe, 1984 a). We recently described 3 mAbs, SST-2, SST-3, and SST-4, reactive with promastigotes of this species (Silveira et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization ofLeishmania (Viannia) braziliensismembrane antigens recognized by mAbs SST-2, SST-3, and SST-4

2003

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The immunolocalization of Leishmania (Viannia) braziliensis stage-specific antigens recognized by mAbs was analysed by transmission electron microscopy. The antigen recognized by mAb SST-2 was present at the surface of promastigotes, including the flagellum and flagellar pocket. The reactivity of SST-2 with isolates of different serodemes showed a pronounced microheterogeneity in terms of the number of reactive bands within the low molecular weight range from 24 to 33 kDa. The 180 kDa glycoprotein recognized by mAb SST-3 was present only in the flagellar membrane. SST-3 also recognized multiple discrete bands from 160 to 200 kDa, as observed in several serodemes. In contrast, mAb SST-4, which recognizes a 98 kDa antigen, showed weak labelling on the promastigote surface by transmission electron microscopy and indirect immunofluorescence. Based on Western blotting, indirect immunofluorescence, and solid-phase radioimmunoassay, the antigens recognized by mAbs SST-2, SST-3 and SST-4 were present in all L. (V.) braziliensis analysed, from 7 different serodemes.

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Identification and purification of a 72kDa antigen of Leishmania braziliensis braziliensis present on the surface and in the cytoplasm of the promastigotes and its specific recognition by sera from mucocutaneous leishmaniasis patients

Cited by 5 publications

References 0 publications

Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Comparison of serological and parasitological methods for cutaneous leishmaniasis diagnosis in the state of Paraná, Brazil

Immunological selection for Leishmania (Viannia) braziliensis antigens

Immunolocalization ofLeishmania (Viannia) braziliensismembrane antigens recognized by mAbs SST-2, SST-3, and SST-4

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