The vitellogenin gene family of Aedes aegypti

Hamblin, Martha T.; Marx, Jeffrey L.; Wolfner, Mariana F.; Hagedorn, Henry H.

doi:10.1590/s0074-02761987000700021

Cited by 10 publications

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“…Four genes were previously isolated from an Ae. aegypti genomic DNA library and restriction mapped ( Gemmill et al 1986 ; Hamblin et al 1987 ). Based on the comparisons of the restriction sites present in the four vitellogenin genes, Vg-A1, A2, B, and C, isolated in this study, and the previously mapped clones, the same four vitellogenin genes had been cloned.…”

Section: Resultsmentioning

confidence: 99%

“…Four vitellogenin genes of Ae. aegypti had been previously cloned ( Hamblin et al 1987 ), and one of them, Vg-A1, was sequenced and extensively studied ( Gemmill et al 1986 ; Racioppi et al 1986 , Chen et al 1994 ; Romans et al 1995 ). However, the clones of the other genes were lost.…”

Section: Discussionmentioning

confidence: 99%

“…This is unlike other organisms such as X. laevis ( Germond et al 1984 ), C. elegans ( Spieth et al 1991 ), D. melanogaster ( Wahli 1988 ), and Ae. aegypti ( Hamblin et al 1987 ), in which several members of the vitellogenin gene family exist.…”

Section: Discussionmentioning

confidence: 99%

“…Vitellogenins are precursors of the yolk proteins, which are phosphoglycolipoproteins utilized in oviparous animals to provide nutrition for the developing embryo. Vitellogenins of many animals have multiple vitellogenin genes including the frog, Xenopus laevis ( Germond et al 1984 ), the chicken, Gallus gallus , the nematode, Caenorhabditis elegans ( Spieth et al 1991 ), and several insects including Drosophila melanogaster ( Wahli 1988 ), and Aedes aegypti ( Hamblin et al 1987 ). The gene duplication events that gave rise to these gene families are not well understood.…”

Section: Introductionmentioning

confidence: 99%

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Mosquito Vitellogenin Genes: Comparative Sequence Analysis, Gene Duplication, and the Role of Rare Synonymous Codon Usage in Regulating Expression

Isoe¹,

Hagedorn²

2007

Journal of Insect Science

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Comparative sequence analysis of mosquito vitellogenin (Vg) genes was carried out to gain a better understanding of their evolution. The genomic clones of vitellogenin genes were isolated and sequenced from all three subfamilies of the family Culicidae including Culicinae (Aedes aegypti, Ochlerotatus atropalpus, Ae. polynesiensis, Ae. albopictus, Ochlerotatus triseriatus and Culex quinquefasciatus), Toxorhynchitinae (Toxorhynchites amboinensis), and Anophelinae (Anopheles albimanus). Genomic clones of vitellogenin genes Vg-B and Vg-C were isolated from Ae. aegypti and sequenced. A comparison of Vg-B and Vg-C, with the previously characterized vitellogenin gene, Vg-A1, suggests that Vg-A1 and Vg-B probably arose by a recent gene duplication, and Vg-C apparently diverged from the two other members of the gene family in an earlier gene duplication event. Two vitellogenin genes orthologous to Vg-C were cloned from a Cx. quinquefasciatus DNA library, one of which is truncated at the N-terminal end. Single vitellogenin genes, orthologous to Vg-C, were cloned from the An. albimanus and Tx. amboinensis libraries. Incomplete sequences orthologous to Vg-B and Vg-C were isolated from the Oc. atropalpus library. Only partial sequences were isolated from Ae. polynesiensis, Ae. albopictus and Oc. triseriatus. Inferred phylogenetic relationships based on analysis of these sequences suggest that Vg-C was the ancestral gene and that a recent gene duplication gave rise to Vg-A1 and Vg-B after the separation of the genus Aedes.The deduced amino acid composition of mosquito vitellogenin proteins exhibits higher tyrosine and phenylalanine composition than other mosquito proteins except for the hexamerin storage proteins. Analysis of vitellogenin coding sequences showed that a majority of amino acid substitutions were due to conserved and moderately conserved changes suggesting that the vitellogenins are under moderately selective constrains to maintain tertiary structure. The vitellogenin genes of the three anautogenous mosquitoes, that require a blood meal to develop eggs, had very high synonymous codon usage biases similar to highly expressed genes of other organisms. On the other hand, the vitellogenin genes of autogenous mosquitoes, that develop at least one batch of eggs without a blood meal, exhibited low synonymous codon usage bias. An unusual pattern of synonymous codon usage was observed in the first 15 amino acid residues encoding the signal peptide in the vitellogenin genes, where a high number of rarely used synonymous codons are present. It is hypothesized that rare synonymous codons have selectively accumulated in the signal peptide region to down-regulate the rate of translation initiation in the absence of a blood meal. Real-time PCR gene expression experiments showed that all three Ae. aegypti vitellogenin genes were highly expressed after a blood meal, and expressed in non-blood-fed females, males, larvae and pupae at trace levels. Sequences were deposited in GenBank (accession numbers: Ae. aegypti Vg-B, AY380797, V...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mosquito Vitellogenin Genes: Comparative Sequence Analysis, Gene Duplication, and the Role of Rare Synonymous Codon Usage in Regulating Expression

Isoe¹,

Hagedorn²

2007

Journal of Insect Science

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“…Multiple vitellogenin genes in the genome of Ae. aegypti have been described ( Hamblin et al 1987 ) while the multiple transcripts with similarity to cathepsin B and vitellogenic carboxypeptidase indicate that these proteins may be encoded by more than one gene as well. It has been already established that vitellogenin, vitellogenic carboxypeptidase and cathepsin B are synthesized by the fat bodies in response to a blood meal, secreted into the hemolymph and accumulate in ovaries, where they are deposited in the oocytes during development ( Raikhel, 1987 ; Raikhel, et al, 2002 ; Raikhel and Lea, 1983 ; Ribeiro, 2003 ).…”

Section: Resultsmentioning

confidence: 99%

A transcriptome analysis of the Aedes aegypti vitellogenic fat body

Feitosa

Calvo

Merino

et al. 2006

Journal of Insect Science

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Aedes (Stegomyia) aegypti is an important dengue vector in tropical and subtropical zones throughout the world. A transcriptome of Ae. aegypti vitellogenic fat bodies is described here. The fat body is a dynamic tissue that participates in multiple biochemical functions of intermediate metabolism. A total of 589 randomly selected cDNAs were assembled into 262 clusters based on their primary sequence similarities. The putative translated proteins were classified into categories based on their function in accordance with significant similarity using the BlastX at NCBI FTP site and Pfam (Bateman et al. 2000) and SMART (Schultz et al. 2000) databases. The characterization of transcripts expressed in the fat body of Ae. aegypti at 24 hours post blood meal provides a basic tool for understanding the processes occurring in this organ and could identify putative new genes whose promoters can be used to specifically express transgenes in the fat bodies of Ae. aegypti.

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Vitellogenesis in Mosquitoes

Raikhel

1992

Advances in Disease Vector Research

100

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The vitellogenin gene family of Aedes aegypti

Cited by 10 publications

References 0 publications

Mosquito Vitellogenin Genes: Comparative Sequence Analysis, Gene Duplication, and the Role of Rare Synonymous Codon Usage in Regulating Expression

Mosquito Vitellogenin Genes: Comparative Sequence Analysis, Gene Duplication, and the Role of Rare Synonymous Codon Usage in Regulating Expression

A transcriptome analysis of the Aedes aegypti vitellogenic fat body

Vitellogenesis in Mosquitoes

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