Endocytosis-inducer adhesins produced by enteropathogenic serogroups of Escherichia coli participate on bacterial attachment to infant enterocytes

Andrade, João Ramos Costa; Silva, Carla Cavalheiro da

doi:10.1590/s0074-02761987000100015

Cited by 5 publications

(5 citation statements)

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“…Ten of the eleven complement factors identified increased, with four having a significant increase (p < 0.05), and only one slightly decreased. These findings were consistent with the previous study by Stabile et al, which showed that C3, C4, and Factor H had the same change during human fetal serum development [33]. For instance, C3, which plays a central role in the activation of both classical and alternative complement pathways, exhibited a significant increase of more than 10-fold in this study.…”

Section: Resultssupporting

confidence: 94%

A comparison of E15.5 fetus and newborn rat serum proteomes

Wei¹,

Jia²,

Zhu³

et al. 2012

Proteome Sci

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BackgroundSerum proteins carry out several functions in the circulation, including transfer, immunological functions, messenger functions, coagulation, and regulation of homeostasis. To investigate changes in serum proteins that occur during development, the serum proteomes of embryonic 15.5 (E15.5) fetuses and newborn rats were compared using LC-MS/MS.ResultsA total of 958 proteins were identified in the serum of rats at both developmental stages. The serum proteome pattern of newborn rats was compared to E15.5 fetuses by relative quantitation. The expression patterns of hemoglobin subunits were different at the two stages, with most of the subunits having decreased expression in newborn rats compared to E15.5 fetuses. In addition, 8 of 12 apolipoproteins were significantly decreased and 10 of 11 identified complement molecules were increased, with 4 exhibiting a significant increase. Moreover, 11 of 14 of the significantly increased enzyme regulators were inhibitors. The serum proteome patterns of different littermates from both developmental stages were also compared. We found that the levels of many highly abundant serum proteins varied between littermates, and the variations were larger than the variations of the technical control.ConclusionsThe serum proteomes of newborn rats and E15.5 fetuses were compared. The expression patterns of hemoglobin subunits were different at the two developmental stages, with most of the subunits having decreased expression. The majority of apolipoproteins had significantly decreased expression, while almost all identified complement proteins had increased expression. The levels of several highly abundant serum proteins also varied among littermates at these two developmental stages. This is the first study using LC-MS/MS to investigate serum proteome development.

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Section: Resultssupporting

confidence: 94%

A comparison of E15.5 fetus and newborn rat serum proteomes

Wei¹,

Jia²,

Zhu³

et al. 2012

Proteome Sci

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“…We showed previously that EPEC with type lpili adhered more readily to HeLa and HEp-2 cells (Andrade et al, 1984;Andrade and Santa Rosa, 1986b) and to human enterocytes (Andrade and Silva, 1987) than non-pilate EPEC strains. Hydrophobic properties and the spatial arrangement of type-1 pili could facilitate the initial contact with the cell surface, improving further interaction between endocytosis-inducer adhesins and their…”

Section: Discussionmentioning

confidence: 70%

“…Adherence is specifically blocked by galactose, glucose and rhamnose and even among EPEC strains of the same serogroup a great diversity of adhesins is anticipated from sugar-inhibition adherence tests (Andrade and Santa Rosa, 1986b). Such EPEC adhesins were called endocytosis-inducer adhesins to emphasise their contribution to bacterial pene- tration into cultured cells (Andrade and Silva, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, jejunal enterocytes from infants have specific receptors for EPEC endocytosis-inducer adhesins, since the binding of some EPEC strains to enterocytes is specifically inhibited by carbohydrates that block attachment to and internalisation of the same strains by HEp-2 cells (Andrade and Silva, 1987). Thus, infection of HEp-2 cells by EPEC seems to provide a useful and reliable model for testing EPEC attachment to and penetration of enterocytes.…”

Section: Discussionmentioning

confidence: 99%

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An endocytic process in HEp-2 cells induced by enteropathogenic Escherichia coli

Andrade

Daveiga²,

rosa³

et al. 1989

Journal of Medical Microbiology

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Summary. Infection of HEp-2 cells by enteropathogenic Escherichia coli (EPEC) was examined by transmission and scanning electronmicroscopy. EPEC strains of serogroups 0 1 1 1 : K58 and 055 : K59 recently isolated from human patients did not exhibit enterotoxic activity, as judged by the Vero-cell and suckling-mouse assays, or invasive ability as judged by the Sereny test. These strains attached to and penetrated HEp-2 cells. Transmission electronmicroscopy showed bacteria in close contact with cell membranes 15 min after infection; later, intense swelling and budding of membranes and penetration of EPEC into the cell cytoplasm occurred. Intracellular bacteria were enclosed in membrane-bound vacuoles in the cell cytoplasm underlying localised adherence sites observed by light microscopy. Scanning electronmicroscopy showed morphologically altered membranes only at the sites of bacterial attachment. Bacteria inactivated by ultraviolet light were not internalised and cytochalasin B ( 2 10 mg/L) markedly inhibited uptake. These observations suggest that penetration of EPEC into HEp-2 cells occurs by an endocytic process in metabolically active bacteria.

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“…Resumidamente, podemos considerar que as crianças estudadas com diarréia aguda associada à EPEC apresentaram como características gerais a baixa idade, peso insuficiente ao nascer, desmame precoce e desnutrição protéico-calórica. Esse perfil vem sendo apontado por vários autores nos últimos anos [23][24][25] . Pudemos observar que os pacientes portadores do sorogrupo O55 tinham idade significativamente menor que aqueles em que se isolou o sorogrupo O111.…”

Section: Discussionunclassified

Diarréia aguda grave associada à Escherichia coli enteropatogênica clássica (EPEC): características clínicas e perdas fecais em lactentes hospitalizados

Oliva¹,

Scaletsky²,

Morais³

et al. 1997

Rev. Assoc. Med. Bras.

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INTRODUÇÃODiarréia aguda é uma síndrome prevalente na infância e observada com maior freqüência nas crianças de tenra idade 1,2 . O impacto dessa síndrome na morbidade é tanto maior quanto menor a idade das crianças acometidas, seja no número de episó-dios por criança por ano, seja nas taxas de hospitalização [3][4][5][6][7] . Diversos estudos realizados em centros urbanos de países em desenvolvimento têm mostra-

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Endocytosis-inducer adhesins produced by enteropathogenic serogroups of Escherichia coli participate on bacterial attachment to infant enterocytes

Cited by 5 publications

References 0 publications

A comparison of E15.5 fetus and newborn rat serum proteomes

A comparison of E15.5 fetus and newborn rat serum proteomes

An endocytic process in HEp-2 cells induced by enteropathogenic Escherichia coli

Diarréia aguda grave associada à Escherichia coli enteropatogênica clássica (EPEC): características clínicas e perdas fecais em lactentes hospitalizados

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