Caracterização biológica comparativa das cepas Berenice e Berenice-78 de Trypanosoma cruzi isoladas da mesma paciente em diferentes períodos

Abstract

The Berenice-78 strain of T. cruzi is very different from the Berenice strain isolated 16 years earlier from the same patient. The authors verified its high infectivity and low virulence for C3H inbred mice that survived the acute phase of infection. In these animals, it was verified that the tropism of parasites was more accentuated for cardiac and skeletal musculature and the parasitaemic level progressively increased with successive blood passages with posterior stability. In relation to Berenice strain the same characteristics were observed as described by Brener, Chiari & Alvarenga (1974). The increase in its virulence for albino mice was again demonstrated. The authors discussed the possibility of reinfection of the patient called Berenice and the importance of knowledge about T. cruzi strains of low virulence for laboratory animals.

Trypanosoma cruzi high infectivity in vitro is related to cardiac lesions during long-term infection in Beagledogs

“…The morphological shape of blood trypomastigotes from different T. cruzi strains had already been described by Chagas (1909) and confirmed for several other authors (Brener & Chiari 1963, Lana & Chiari 1986, Marques-Araújo & Chiari 1988. Our results are in accordance to those described in the literature for Y strain (Brener & Chiari 1963, Marques-Araújo & Chiari 1988.…”

“…Until today, the pathogenesis of the chronic phase is not fully understood, in part by the difficulty in finding the ideal animal model and by the lack of correlation between the host immune response and heart damage. Since chronic canine chagasic cardiomyopathy has been shown to resemble the human disease (Lana & Chiari 1986, Lana et al 1992 we decided to investigate the correlation between the production of different immunoglobulins isotypes and pathogenesis of Chagas disease.…”

“…cruzi strains -T. cruzi Y strain (T. cruzi II) isolated from an acute case of human Chagas disease (Silva & Nussenzweig 1953) and Berenice-78 strain (T. cruzi II), isolated by xenodiagnosis on 1978 (Lana & Chiari 1986) from the first reported Chagas disease patient, Berenice (Chagas 1909).…”

Activity of the New Triazole Derivative Albaconazole against Trypanosoma ( Schizotrypanum ) cruzi in Dog Hosts

“…cruzi strains. The T. cruzi strains used in this study were T. cruzi Y, which is partially resistant to benznidazole, and T. cruzi II Berenice-78, which is susceptible to benznidazole (12,15,24,35).…”

Variation in susceptibility to benznidazole in isolates derived from Trypanosoma cruzi parental strains

“…cruzi strains and populations isolated from dogs -The parental strains Colombian (Col) -T. cruzi I (Federici et al 1964), resistant to benznidazole (Filardi & Brener 1987) and Berenice-78 (Be-78) -T. cruzi II (Lana & Chiari 1986), 100% susceptible to benznidazole (Toledo et al 1995), were used as references.…”