Vaccination of Callithrix jacchus (Linné, 1758) marmosets with the PF strain of Trypanosoma cruzi

Abstract

Callithrix jacchus marmosets, vaccinated more than once with high doses of the PF strain of Trypanosoma cruzi, showed a certain degree of parasitemia related to the number of parasites injected. Thirty days after vaccination, all animals were alive and showed no apparent morbid symptoms. The relationship between the dose of injected trypanosomes and the observed parasitemias is discussed and analysed as well as the immunologic incompetence of the experimental animals used.<br>Sagüis da espécie Callithrix jacchus vacinados mais de uma vez, com altas doses de tripanosomas da cepa PF do T. cruzi, apresentam certo grau de parasitemia relacionado com o número de parasitas injetados. Trinta dias após a vacinação todos os animais estavam vivos e sem manifestações aparentes da doença. A relação entre a dose de tripanosomas injetados e as parasitemias observadas é discutida e analisada bem como a incompetência imunológica dos animais usados

Evaluation of pathogen P21 protein as a potential modulator of the protective immunity induced by Trypanosoma cruzi attenuated parasites

“…In our experience and from others as well, a subpatent infection with naturally or genetically modified attenuated parasites is enough to confer strong protection against a subsequent infection. 10 , 17 , 18 Hence, if rP21 is described as a determinant protein favoring parasite cell invasion and permanence in the host, we could speculate that the additional supplementation of rP21 during the immunisation process with the attenuated parasites would promote the continuity of the parasites in the host for a longer period of time, thus giving additional time for the development of a prophylactic immune response able to avoid a second parasite establishment. On the other hand, we would be also favoring the recruitment of immune cells in a larger extent being able to improve the protective immune response conferred by the attenuated parasites.…”