Estudos sobre a evolução dos Endotrematodes brazileiros

Lutz, Adolpho

doi:10.1590/s0074-02761924000100003

Cited by 27 publications

(16 citation statements)

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“…However, cercariae of Pa. segregatum recovered from Biomphalaria spp. in Brazil possess refractile granules at the base of the oral sucker (Lutz 1924;Ruiz 1952;Lie and Basch 1967) that are lacking in the larvae of Drepanocephalus spp. Moreover, the numbers of excretory concretions (>40) in the main excretory ducts are higher in cercariae of Pa. segregatum than in the species of Drepanocephalus.…”

Section: Discussionmentioning

confidence: 99%

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

Pinto

Griffin

Quiniou³

et al. 2015

Parasitol Res

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Species of trematodes belonging to the genus Drepanocephalus are intestinal parasites of piscivorous birds, primarily cormorants (Phalachrocorax spp.), and are widely reported in the Americas. During a 4-year malacological study conducted on an urban lake in Brazil, 27-collar-spined echinostome cercariae were found in 1665/15,459 (10.7 %) specimens of Biomphalaria straminea collected. The cercariae were identified as Drepanocephalus spp. by sequencing the 18S (SSU) rDNA, ITS1/5.8S rDNA/ITS2 (ITS), 28S (LSU) rDNA region, cytochrome oxidase subunit 1 (CO1), and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) markers. In experimental life cycle studies, metacercariae developed in laboratory-reared guppies (Poecilia reticulata); however, attempts to infect birds and rodents were unsuccessful. Two closely related morphotypes of cercariae were characterized. One species, identified by molecular markers as a genetic variant of Drepanocephalus auritus (99.9 % similarity at SSU, ITS, LSU; 97.2 % at CO1; 95.8 % at ND1), differs slightly from an archived North American isolate of this species also sequenced as part of this study. A second species, putatively identified as Drepanocephalus sp., has smaller cercariae and demonstrates significant differences from D. auritus at the CO1 (11.0 %) and ND1 (13.6 %) markers. Aspects related to the morphological taxonomic identification of 27-collar-spined echinostome metacercariae are briefly discussed. This is the first report of the involvement of molluscs of the genus Biomphalaria in the transmission of Drepanocephalus and the first report of D. auritus in South America.

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Section: Discussionmentioning

confidence: 99%

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

Pinto

Griffin

Quiniou³

et al. 2015

Parasitol Res

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“…In some cases, life cycles were experimentally established starting from one of the developmental stages collected in nature, using snails and vertebrates from the laboratory for the completion of the cycle. The life cycle of some species, such as E. macrorchis and E. elegans, was completed from materials collected in trapped wild birds as described by Lutz (1924) . Thus, caution should be used with this approach since the finding of small samples of hosts infected with echinostomes in field surveys may lead to false interpretations in relation to the usual natural hosts of such species.…”

Section: Echinostomes As Experimental Modelsmentioning

confidence: 99%

“…Echinostoma revolutum has been reported from a large variety of definitive hosts, such as birds, pig, dog, rat, among others (Lutz 1924 ). This may serve as example of the wide range of hosts that an echinostome can use.…”

Section: Experimental Definitive Hosts Of Major Echinostomes Speciesmentioning

confidence: 99%

Echinostomes in the wild

Maldonado

Lanfredi

2008

The Biology of Echinostomes

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“…The natural definitive host is the wild semi-aquatic rodent Nectomys squamipes (Maldonado et al 2001). Echinostomes have been found parasitizing livestock, such as pigs, chickens and ducks, as well as pets (Lutz 1924;Maldonado and Lanfredi 2009). The adult worms of Echinostoma have been found in the gastrointestinal tract of the definitive host (Nollen 1996).…”

Section: Introductionmentioning

confidence: 99%

Effect of praziquantel on adult Echinostoma paraensei worms in experimentally infected mice

et al. 2012

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Echinostomiasis is a food-borne intestinal, snail-mediated parasitosis caused principally by ingestion of snails infected with digenean trematodes of the Echinostoma genus. The treatment and control of trematodiasis is usually done by administration of praziquantel (PZQ). In this study, we evaluated the effect on Echinostoma paraensei of different doses of praziquantel through analysis of morphological parameters using light microscopy, scanning electron microscopy, and confocal scanning laser microscopy along with parasitological data. We used 30 female mice aged 4 weeks. Each animal was given 40 metacercarie of E. paraensei by gavage. The animals were divided into five groups, each group containing six animals, where one group was utilized as untreated control. Two weeks after infection, the mice were given praziquantel by gavage at total dosages of 12.5, 25, 50 or 100 mg/kg by body weight. Two days after treatment, the mice were euthanized in a CO(2) chamber for recovery of helminths in the small intestine. The doses of 50 and 100 mg/kg of praziquantel eliminated all the worms. There were significant differences (p<0.05) between all the treated groups when compared to the control group. The body morphology showed contraction with vacuolization of the parenchyma, and the spine of the peristomic collar was not evident by light microscopy. The scanning electron microscopy revealed that the other doses caused retraction of spines of the peristomic collar and also the tegument spines at the body edge, as well as the development of vesicles and peeling; all these alterations were more evident at the dose of 25 mg/kg. In turn, the confocal scanning laser microscopy revealed vacuolization and disorganization of spines and vitelline glands. E. paraensei responds differently to experimental treatment with praziquantel according to the doses utilized causing morphological alteration and even worm elimination.

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Estudos sobre a evolução dos Endotrematodes brazileiros

Cited by 27 publications

References 0 publications

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

Biomphalaria straminea (Mollusca: Planorbidae) as an intermediate host of Drepanocephalus spp. (Trematoda: Echinostomatidae) in Brazil: a morphological and molecular study

Echinostomes in the wild

Effect of praziquantel on adult Echinostoma paraensei worms in experimentally infected mice

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