Gonadal hybrid dysgenesis in Drosophila sturtevanti (Diptera, Drosophilidae)

Almeida, Luciane Madureira; Carareto, Cláudia Márcia Aparecida

doi:10.1590/s0073-47212002000200007

Cited by 8 publications

(4 citation statements)

References 30 publications

(30 reference statements)

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“…Together with the bi-directionality of the low hybrid dysgenesis indices previously reported for this species (Almeida, 2000;Almeida and Carareto, 2002), this statistical homogeneity allows us to reinforce the suggestion of P element inactivity in D. sturtevanti. These findings also contribute to our knowledge of the structural differentiation and geographical variation in P elements of D. sturtevanti.…”

Section: P Element Rflp Analysissupporting

confidence: 86%

Geographic polymorphism of P element in populations of Drosophila sturtevanti

2003

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The aim of this report was to detect full-sized P element sequences in eight strains of Drosophila sturtevanti populations from distant geographic regions and to assess the structural geographic variation among P element sequences. PCR analysis confirmed the presence of a putative complete P element in all strains. Southern blot analysis indicated bands shared by all strains, and bands restricted to geographically related strains. Parsimony analysis corroborated the hybridization pattern that reflected the geographic relationships.

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Section: P Element Rflp Analysissupporting

confidence: 86%

Geographic polymorphism of P element in populations of Drosophila sturtevanti

2003

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“…The morphology of the male gonads of the F1 hybrids was analyzed under a stereomicroscope microscope (model MZ APO; Leica Microsystems GmbH, Wetzlar, Germany) fitted with the Motic Advanced 3.2 Plus Image Analysis System (Motic, Hong Kong) to evaluate the presence of gonadal dysgenesis (which may be uni- or bilateral) [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Trends in evolution of the Rhodniini tribe (Hemiptera, Triatominae): experimental crosses between Psammolestes tertius Lent & Jurberg, 1965 and P. coreodes Bergroth, 1911 and analysis of the reproductive isolating mechanisms

et al. 2021

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Background The tribe Rhodniini is a monophyletic group composed of 24 species grouped into two genera: Rhodnius and Psammolestes. The genus Psammolestes includes only three species, namely P. coreodes, P. tertius and P. arthuri. Natural hybridization events have been reported for the Rhodniini tribe (for genus Rhodnius specifically). Information obtained from hybridization studies can improve our understanding of the taxonomy and systematics of species. Here we report the results from experimental crosses performed between P. tertius and P. coreodes and from subsequent analyses of the reproductive and morphological aspects of the hybrids. Methods Crossing experiments were conducted between P. tertius and P. coreodes to evaluate the pre- and post-zygotic barriers between species of the Rhodniini tribe. We also performed cytogenetic analyses of the F1 hybrids, with a focus on the degree of pairing between the homeologous chromosomes, and morphology studies of the male gonads to evaluate the presence of gonadal dysgenesis. Lastly, we analyzed the segregation of phenotypic characteristics. Results Interspecific experimental crosses demonstrated intrageneric genomic compatibility since hybrids were produced in both directions. However, these hybrids showed a high mortality rate, suggesting a post-zygotic barrier resulting in hybrid unviability. The F1 hybrids that reached adulthood presented the dominant phenotypic segregation pattern for P. tertius in both directions. These insects were then intercrossed; the hybrids were used in the cross between P. tertius ♀ × P. coreodes ♂ died before oviposition, and the F1 hybrids of P. coreodes ♀ x P. tertius ♂ oviposited and their F2 hybrids hatched (however, all specimens died after hatching, still in first-generation nymph stage, pointing to a hybrid collapse event). Morphological analyses of male gonads from F1 hybrids showed that they did not have gonadal dysgenesis. Cytogenetic analyses of these triatomines showed that there were metaphases with 100% pairing between homeologous chromosomes and metaphases with pairing errors. Conclusion The results of this study demonstrate that Psammolestes spp. have intrageneric genomic compatibility and that post-zygotic barriers, namely unviability of hybrid and hybrid collapse, resulted in the breakdown of the hybrids of P. tertius and P. coreodes, confirming the specific status of species based on the biological concept of species. Graphical abstract

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“…This result can be confirmed by the birth of hybrids in all directions of the crossings (Table 1), which demonstrates that both gonads and gametogenesis are normally occurring in first generation hybrids (F1). Gonadal dysgenesis, a phenomenon quite common in drosophilids [75], has never been reported in the subfamily Triatominae. On the other hand, the sterility of the hybrid from the abnormal gametogenesis (chromosomal pairing errors) has been observed several times in these vectors [58,76,77].…”

Section: Plos Onementioning

confidence: 97%

Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex

et al. 2021

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Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.

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Gonadal hybrid dysgenesis in Drosophila sturtevanti (Diptera, Drosophilidae)

Cited by 8 publications

References 30 publications

Geographic polymorphism of P element in populations of Drosophila sturtevanti

Geographic polymorphism of P element in populations of Drosophila sturtevanti

Trends in evolution of the Rhodniini tribe (Hemiptera, Triatominae): experimental crosses between Psammolestes tertius Lent & Jurberg, 1965 and P. coreodes Bergroth, 1911 and analysis of the reproductive isolating mechanisms

Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex

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