2012
DOI: 10.1590/s0044-59672012000200017
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Abstract: Este estudo avaliou a eficácia da heparina sódica e do EDTA tripotássico como anticoagulantes e seus efeitos sobre os parâmetros hematológicos de tambaqui (Colossoma macropomum). Foram utilizados dez indivíduos de tambaqui com médias de 384,9 ± 85,71 g de peso e 27,90 ± 2,10 cm de comprimento total para avaliação da heparina 5.000 UI e 100 UI, bem como do K 3 EDTA 10%. Foram analisados a inibição da coagulação por 10 h, eritrograma e teste de fragilidade osmótica dos eritrócitos. Os resultados foram submetidos… Show more

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Cited by 5 publications
(3 citation statements)
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References 13 publications
(8 reference statements)
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“…Walencik & Witeska (2007) observed higher fragility in common carp erythrocytes to hipotonic solution since the lesser concentration of Na 2 EDTA tests (0.01mg ml -1 ) and a positive relation of the osmotic fragility and the increase of the anticoagulant concentrations. Similar results were described in Blennius pholis (Mainwaring & Rowley 1985), Oncorhynchus mykiss (Korcock et al 1988), Oreochromis niloticus (Ekanem et al 2012), Pseudoplatystoma reticulatum x P. corruscans (Ishikawa et al 2010) and Colossoma macropomum (Pádua et al 2012).…”
Section: Discussionsupporting
confidence: 77%
See 1 more Smart Citation
“…Walencik & Witeska (2007) observed higher fragility in common carp erythrocytes to hipotonic solution since the lesser concentration of Na 2 EDTA tests (0.01mg ml -1 ) and a positive relation of the osmotic fragility and the increase of the anticoagulant concentrations. Similar results were described in Blennius pholis (Mainwaring & Rowley 1985), Oncorhynchus mykiss (Korcock et al 1988), Oreochromis niloticus (Ekanem et al 2012), Pseudoplatystoma reticulatum x P. corruscans (Ishikawa et al 2010) and Colossoma macropomum (Pádua et al 2012).…”
Section: Discussionsupporting
confidence: 77%
“…The blood coagulation is one of the main causes of the quality deterioration of a hematological analysis, also being responsible for alterations in the immunological and biochemical parameters (Pádua et al 2012). The most used anticoagulants for fish are the sodium heparin and the sodium ethylendiamintetracetic acid (NA 2 EDTA).…”
Section: Introductionmentioning
confidence: 99%
“…At the beginning and at the end of days of the experiment, nine sh in total (N= 9) were captured with nets (n= 9 sh per diet; N= 54) after fasting for 24 hours. The juvenile tambaqui collected were anesthetized with benzocaine (100 mg.L -1 ), for blood collection (1 mL per sh) by puncturing the caudal vessel, mediated by 1 mL syringes, 13 x 4.00 mm needles, containing 15 μL of the anticoagulant Heparin sodium 5,000 IU (150 IU.mL -1 of blood) diluted in 0.65% saline solution (1:50) (Pádua et al 2012;Oliveira et al 2012).…”
Section: Sample Collection Biometrics and Blood Parametersmentioning
confidence: 99%