Efeito de diferentes reguladores de crescimento na regeneração in vitro de pau-rosa (Aniba rosaeodora Ducke)

Jardim, Lyana Silva; Sampaio, Paulo de Tarso Barbosa; Costa, Suely de Souza; Gonçalves, Cláudia de Queiroz Blair; Brandão, Hélio Leonardo Moura

doi:10.1590/s0044-59672010000200005

Cited by 15 publications

(11 citation statements)

References 1 publication

(2 reference statements)

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“…Neste trabalho, percebeu-se a formação de brotos nos explantes de T. guianensis, mesmo na ausência de reguladores vegetais (Figura 2), o mesmo sendo observado para Aniba rosaeodora Ducke (Jardim et al, 2010) e Syzygium cordatum (Dewir et al, 2011 (Bhatt & Dhar, 2004), nos quais não foi observada a iniciação do processo morfogenético, em meio de cultura ausente de reguladores. Essa maior capacidade morfogenética de alguns tecidos pode ser causada pela variabilidade genética de cada cultura (George & Debergh, 2008).…”

Section: Resultsunclassified

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

et al. 2013

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In vitro multiplication of Tapirira guianensis Aubl. (Anacardiaceae)Tapirira guianensis is of great medicinal, ecological and socio-economical importance occurring throughout the Brazilian territory. The objective of this study was to establish and determine the best conditions for the in vitro multiplication of T. guianensis. The explants nodal, cotyledonary and epicotyl segments from seedlings germinated in vitro were tested in diverse concentrations of 6-benzilaminopurine (BAP) and/or napthaleneacetic acid (NAA) in medium WPM. The following parameters were evaluated: the percentage of responsive explants, the number of shoots and buds, and the length of shoots and shoot dry mass at 30 and 60 days after inoculation. The cotyledonary segment, under these conditions, is the most suitable explant for the multiplication, with no induction of adventitious buds in epicotyl. The treatment with 1.0 mg L -1 BAP in the absence of NAA is the most responsive to the T. guianensis regeneration.Key words: medicinal woody, in vitro propagation, regeneration, direct organogenesis. Tapirira guianensis possui grande relevância medicinal, ecológica e socioeconômica, ocorrendo em todo o territó-rio brasileiro. O objetivo deste estudo foi estabelecer e determinar as melhores condições para a sua multiplicação in vitro. Os explantes, segmentos nodais, cotiledonares e epicótilos, oriundos de plântulas germinadas in vitro, foram testados em concentrações de 6-benzilaminopurina (BAP) e, ou, ácido naftalenoacético (ANA), em meio de cultura WPM. As características avaliadas foram a percentagem de explantes responsivos, o número de brotos e de gemas, o comprimento dos brotos e a matéria seca da parte aérea, aos 30 e 60 dias após inoculação. Foi observado que o segmento cotiledonar, nas condições deste estudo, foi o explante mais indicado para a multiplicação, não havendo indução de brotos adventícios nos epicótilos. O tratamento com 1,0 mg L -1 de BAP na ausência de ANA é o mais responsivo para a regeneração de T. guianensis.Palavras-chave: lenhosa medicinal, propagação in vitro, regeneração, organogênese direta.

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Section: Resultsunclassified

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

et al. 2013

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“…The different combinations between BAP and NAA significantly reduced root induction (figure 2g). Cytokinins added to the culture medium generally promote the formation of adventitious shoots; whereas auxins stimulate the formation of the root system in tissues predisposed to rooting (Jardim et al 2010). C. ferrea explants, when subjected to isolated concentrations of NAA, had a low rooting rate (figure 2g).…”

Section: Literature Citedmentioning

confidence: 99%

In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Silva

Imakawa

Costa

et al. 2018

Hoehnea

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The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.

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“…This stage is even more difficult when the shooting is from indirect organogenesis because the formation of calluses at base of shoots, which may jeopardize the differentiation of roots (MANTOVANI et al, 2010;DIAS;BONDANI, 2013). However, the callus may have either isolated cells or in groups with active centers of cell division, which once properly induced enable the production of new organs (JARDIM et al, 2010), which might explain the rooting of shoots with calluses at the base of explant. However, usually at the acclimatization phase only photosynthesis is playing a role as an energy supplier to the plant from in vitro cultivation.…”

Section: Resultsmentioning

confidence: 99%

Indirect in vitro organogenesis of Hancornia speciosa Gomes

Prudente¹,

Paiva²,

Nery³

et al. 2016

Biosci. J.

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ABSTRACT:Hancornia speciosa Gomes is a fruit species belonging to the Apocynaceae family and holds social, cultural and economic potential mostly due to its use in composition of many food industry products and the consumption its fruits in natura. Several aspects regarding their propagation need further studies, since the species is undergoing a continuous process of domestication. The objective was to obtain an in vitro protocol for indirect organogenesis and rooting with subsequent acclimatization of H. speciosa plants. To obtain indirect organogenesis, internodal segments were inoculated in WPM culture medium gelled with 7 g L -1 agar, added with 30 g L -1 sucrose, 0.4 g L -1 PVP, and supplied with different concentrations of 2,4-D (0.0; 2.46; 7.38; 12.30; and 17.22 µM), BAP (0.0; 4.92; 9.84; 14.76; and 19.68 µM), and TDZ (0.0; 4.92; 9.84; 14.76; and 19.68 µM). For the in vitro rooting, shootings with approximately 6.0 cm diameter length kept for 15 days in WPM medium with no plant growth regulator and, afterwards, subjected to treatments with different auxins (Control; 9.84 µM IBA; 9.84 µM NAA; and 9.84 µM IAA) as well as the combination among them, in order to verify their effects on percentage of rooting (%), number of roots, and average length of the largest root (cm). The formation of calluses was observed in all explants subjected to the concentrations of the regulators tested. The highest shooting regeneration occurred with 7.38 µM 2,4-D (73%). The highest percentage of shoot rooting (80%) and roots with the largest length (1.3 cm) were found in the culture medium with the combination of 4.92 µM NAA and 4.92 µM IBA. The in vitro regeneration of H. speciosa is feasible. The acclimatization of rooted shoots in Trospstrato® was accomplished with successful and 100% survival of plant material was observed during this stage.

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Efeito de diferentes reguladores de crescimento na regeneração in vitro de pau-rosa (Aniba rosaeodora Ducke)

Cited by 15 publications

References 1 publication

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Indirect in vitro organogenesis of Hancornia speciosa Gomes

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