Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis

Silva, João Guilherme Lino da; Silva, Thiago Miranda da; Peloso, Eduardo de Figueiredo; Machado-Coelho, George Luiz Lins; Mayrink, Wilson; Ariosa, Marília Caixeta Franco; Silva, Paulo Márcio de Faria e; Marques, Marcos José

doi:10.1590/s0037-86822012000200023

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…The association of MST with molecular biology methods, especially in reference treatment centers, offers higher diagnostic accuracy as well as good sensitivity and specificity. Therefore, a combination of diagnostic methods is usually required to obtain accurate results 15 , 16 . There is a need to develop faster, more effective and simpler assays for the diagnosis of this disease 17 .…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Zanetti

Sato

Longhi³

et al. 2019

Rev. Inst. Med. trop. S. Paulo

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American Tegumentary leishmaniasis (ATL) is an infectious disease caused by several species of Leishmania . Even though the direct detection of parasites has low sensitivity, it is still the gold standard for the laboratory diagnosis of ATL. Recent studies have shown promising results of Enzyme-Linked Immunosorbent Assays ( ELISAs) using recombinant antigens. The aim of this study is to compare the accuracy of ELISAs using novel antigens with the standard ELISA based on soluble antigens of Leishmania (SLA) to diagnose ATL. Studies that analyzed patients with ATL and studies that evaluated the diagnostic accuracy of ELISAs using novel antigens and SLA were included. The Fourteen studies from PubMed, Regional Portal of the Virtual Health Library (BVS), Brazilian Society of Dermatology, Virtual Health Library (IBECS), Literature in the Health Sciences in Latin America and the Caribbean (LILACS), Medical Literature Analysis and Retrieval System Online (Medline), Elsevier Embase, Cochrane Library, The National Institute for Health and Care Excellence (NICE), and Cumulative Index to Nursing and Allied Health Literature (CINAHL) were included. The novel ELISA antigens showed a high sensitivity (93.8%-100%) and specificity (82.5-100%), a better diagnostic performance than SLA-based ELISAs (1-97.4% and 57.5-100%, respectively). Only 10 studies analyzed cross-reactions in serum samples from patients with Chagas disease, and only two studies reported a percentage of cross-reactivity. In this systematic review, the novel ELISA antigens showed better sensitivity and specificity with respect to SLA-based ELISAs. However, a meta-analysis should be performed to confirm this finding.

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Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Zanetti

Sato

Longhi³

et al. 2019

Rev. Inst. Med. trop. S. Paulo

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“…At the end of the selection process, 14 papers were included based on the above-mentioned criteria ( Fig. 1 , Supplementary data II): Piñero et al (1999), Pirmez et al (1999) , Victoir et al (2003) , Disch et al (2005) , Camera et al (2006) , Bracho et al (2007) , Deborggraeve et al (2008) , Pereira et al (2008) , Fagundes et al (2010), Boggild et al (2011) , Thomaz-Soccol et al (2011) , Veland et al (2011) , Marco et al (2012) and Neitzke-Abreu et al (2013) .…”

Section: Resultsmentioning

confidence: 99%

Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis

Gomes¹,

Mazin²,

Santos³

et al. 2015

Mem. Inst. Oswaldo Cruz

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The diagnosis of mucocutaneous leishmaniasis (MCL) is hampered by the absence of a gold standard. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. This study aimed to assess the ability of polymerase chain reaction (PCR) to identify MCL and to compare these results with clinical research recently published by the authors. A systematic literature review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses: the PRISMA Statement was performed using comprehensive search criteria and communication with the authors. A meta-analysis considering the estimates of the univariate and bivariate models was performed. Specificity near 100% was common among the papers. The primary reason for accuracy differences was sensitivity. The meta-analysis, which was only possible for PCR samples of lesion fragments, revealed a sensitivity of 71% [95% confidence interval (CI) = 0.59; 0.81] and a specificity of 93% (95% CI = 0.83; 0.98) in the bivariate model. The search for measures that could increase the sensitivity of PCR should be encouraged. The quality of the collected material and the optimisation of the amplification of genetic material should be prioritised.

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“…The same work demonstrated the high concordance between kDNA and SSU rDNA, concluding that both may be used for American CL diagnosis; altogether, the results indicate the best performance of the kDNA protocol. Summarizing, the kDNA target may be used for diagnosis due to its sensitivity [ 78 , 79 , 87 , 103 – 105 ], whereas ITS-1 is more specific and may be used for species identification [ 78 ].…”

Section: Molecular Tools For Leishmaniases Diagnosismentioning

confidence: 99%

Leishmaniases diagnosis: an update on the use of immunological and molecular tools

et al. 2015

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Leishmaniases are caused by obligate intracellular protozoan parasites of the genus Leishmania. They cause a spectrum of diseases, most notably visceral (VL), cutaneous (CL), and mucosal (ML) leishmaniasis, which affect millions of people around the world, each year. Despite scientific advances, leishmaniases cases are expanding, constituting an important public health problem. Immunological and molecular diagnostic tools have been increasingly applied for the early detection of these parasitic infections, since the existence of limitations in clinical and parasitological examinations may provide false results, thus interfering in epidemiological research and diseases control. Although there is a great diversity of available immunological assays, important common deficiencies persist, which explains the current exploration of the molecular biology in research fields, especially the Polymerase Chain Reaction (PCR) and its variants, such as real-time quantitative PCR. However, in the last years, significant results have also been reached inside of immunological context (especially by Flow Cytometry), for humans and dogs, demonstrated by research works of the New and Old worlds. In spite of their potential to clarify and minimize the present global situation of the diseases, the implementation of molecular or immunological innovative reference assays for VL and CL at health services is still a challenge due to several reasons, including lack of standardization among laboratories and structural concerns. In this article we bring classical and current information about technological advances for the immunological and molecular leishmaniases diagnosis, their features, and applications.

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Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with american cutaneous leishmaniasis

Cited by 5 publications

References 13 publications

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Diagnostic accuracy of Enzyme-Linked Immunosorbent Assays to detect anti-Leishmania antibodies in patients with American Tegumentary Leishmaniasis: a systematic review

Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis

Leishmaniases diagnosis: an update on the use of immunological and molecular tools

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