Seroepidemiological monitoring in sentinel animals and vectors as part of arbovirus surveillance in the state of Mato Grosso do Sul, Brazil

Abstract

Introduction: From February-September 2010, seroepidemiological surveys were conducted on non-human primates and transmitter vector capture was used to investigate the possible circulation of arboviruses in the municipalities of Bonito, Campo Grande, and Jardim, State of Mato Grosso do Sul, Brazil. Methods: A total of 65 primates from the wild and captivity were used, and potential vectors were captured using Castro and dip nets. Serum samples were tested at the Instituto Evandro Chagas, State of Pará, using the hemagglutination inhibition test to detect total antibodies against 19 different arboviruses. Virus isolation was attempted from serum samples and arthropod suspensions using newborn mice and the C6/36 cell line clone. In addition, identification of the vector species was conducted. Results: From the 19 serum samples from Campo Grande, 1 sample had a 1:20 titer for Flavivirus. From the 35 samples collected in Bonito, 17 samples had antibodies to arboviruses, 4 (11.4%) were positive for Alphavirus, and 5 (14.2%) were positive for Flavivirus. Monotypic reactions were observed for the Mayaro (n = 10) and Oropouche (n = 5) viruses, and 6 (17.1%) samples had titers for >1 virus. We captured 120 Culicidae individuals that were potential arbovirus transmitters in Jardim; however, all the samples were negative for the viruses. Conclusions: Mato Grosso do Sul has a variety of vertebrate hosts and transmission vectors, thereby providing ideal conditions for the emergence or reemergence of arboviruses, including some pathogenic to human beings.

Oropouche virus is detected in peripheral blood leukocytes from patients

“…Recently, the S segment of OROV was detected in sera from patients with acute febrile illness and in pools of Culex quinquefasciatus in the urban area of Cuiabá and in another nearby county in the state of Mato Grosso, also within the Cerrado biome, and thus away from the Amazon forest [Cardoso et al, ]. Serological surveys suggest that OROV may circulate in a range of wild and domestic animals, including species of birds and primates from the Amazon [Pinheiro et al, ; Pinheiro et al, ] and primates from Southeast and South of Brazil [Cardoso Jda et al, ; Batista et al, ; Batista et al, ]. Additionally, positive serology for OROV was reported in the city of Ribeirao Preto, state of São Paulo, Southeast of Brazil [Figueiredo et al, ].…”

Detection of arboviruses of public health interest in free-living New World primates (Sapajus spp.; Alouatta caraya) captured in Mato Grosso do Sul, Brazil

“…Arboviruses are considered to be emerging disease agents when they initially appear in a population or when their incidence or geographic distribution increases considerably 3 , as observed in a recent serological study of non-human primates (NHPs) in the municipality of Bonito, Mato Grosso do Sul, which detected antibodies to MAYV and OROV 4 . A number of factors can precipitate emerging disease, such as ecological changes due to economic development, agricultural or climatic abnormalities, demographic and behavioral changes, international traffi c and wildlife trade, microbial adaptation or a collapse in public health control programs 3 .…”

Prolonged polyarthralgia in a German traveller with Mayaro virus infection without inflammatory correlates

“…The virus is transmitted by bites of Haemagogus janthinomys which lives in the forest canopy and propagates in a sylvatic cycle predominantly in monkeys [16,17]. Hence, working and living in the Amazon rain forest is the most important risk factor for the acquisition of MAYV infection [12,13,18]. Clearly, our patient was at risk while working in a wildlife resort.…”