Molecular detection of Treponema pallidum sp. pallidum in blood samples of VDRL-seroreactive women with lethal pregnancy outcomes: a retrospective observational study in northern Brazil

Casal, Charliana Aragão Damasceno; Silva, Mayra Oliveira da; Costa, Igor Brasil; Araújo, Eliete da Cunha; Corvelo, Tereza Cristina de Oliveira

doi:10.1590/s0037-86822011005000047

Cited by 15 publications

(20 citation statements)

References 28 publications

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“…Newborns of mothers with syphilis who are untreated or inadequately treated may be asymptomatic. This can lead to the absence of diagnosis and treatment, causing serious damage to their health, with psychological and social repercussions 6 .…”

Section: Introductionmentioning

confidence: 99%

Risk factors for syphilis in women: case-control study

et al. 2017

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OBJECTIVE To determine the sociodemographic, behavioral, and health care factors related to the occurrence of syphilis in women treated at public maternity hospitals.METHODS This is a case-control study (239 cases and 322 controls) with women admitted to seven maternity hospitals in the municipality of Recife, Brazil, from July 2013 to July 2014. Eligible women were recruited after the result of the VDRL (Venereal Disease Research Laboratory) under any titration. The selection of cases and controls was based on the result of the serology for syphilis using ELISA (enzyme-linked immunosorbent assay). The independent variables were grouped into: sociodemographic, behavioral, clinical and obstetric history, and health care in prenatal care and maternity hospital. Information was obtained by interview, during hospitalization, with the application of a questionnaire. Odds ratios and 95% confidence intervals were estimated using logistic regression to identify the predicting factors of the variable to be explained.RESULTS The logistic regression analysis identified as determinant factors for gestational syphilis: education level of incomplete basic education or illiterate (OR = 2.02), lack of access to telephone (OR = 2.4), catholic religion (OR = 1.70 ), four or more pregnancies (OR = 2.2), three or more sexual partners in the last year (OR = 3.1), use of illicit drugs before the age of 18 (OR = 3.0), and use of illicit drugs by the current partner (OR = 1.7). Only one to three prenatal appointments (OR = 3.5) and a previous history of sexually transmitted infection (OR = 9.7) were also identified as determinant factors.CONCLUSIONS Sociodemographic, behavioral, and health care factors are associated with the occurrence of syphilis in women and should be taken into account in the elaboration of universal strategies aimed at the prevention and control of syphilis, but with a focus on situations of greater vulnerability.

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Section: Introductionmentioning

confidence: 99%

Risk factors for syphilis in women: case-control study

et al. 2017

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“…Among women with fetal loss, a higher frequency of detection of bacterial DNA was recorded in mothers with early (active) syphilis 11. A significant association was found between stillbirths and early maternal syphilis, and miscarriages and latent maternal syphilis.…”

Section: Discussionmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

“…The serum samples were submitted to the FTA-Abs IgG, ELISA IgG and FTA-Abs IgM tests, a procedure described by Casal et al 11. The DNA of T pallidum was detected using a nested PCR (nPCR) to amplify an external region of 291 bp described by Casal et al 11 and an internal region of 121 bp of the polA gene. The second primer pair was based on an internal region of the PCR product obtained in the first amplification of the polA gene and consisted of a 19-mer forward primer (5 ′ -TTAAAAGAAGCGCTGCGCA-3 ′ ) and a 22-mer reverse primer (5 ′ -TACAACAGGAATCTTCGAGCGA-3 ′ ).…”

Section: Methodsmentioning

confidence: 99%

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Risk factors and pregnancy outcomes in women with syphilis diagnosed using a molecular approach

Casal¹,

Araújo

Corvelo

2012

Sex Transm Infect

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“…The sensitivity and specificity of the molecular PCR test results are affected by the type of specimen, the PCR method, and the target gene used. (10,11) Specimens that may be used for the Treponema pallidum PCR test in secondary syphilis are exudates from syphilitic lesions and blood. The sensitivity of PCR with smears from lesions of secondary syphilis reaches 80% and the specificity 98.6%.…”

Section: Introductionmentioning

confidence: 99%

Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum

et al. 2018

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UNIVERSA MEDICINAMultiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum Ida Effendi* , **, Yeva Rosana**, Andi Yasmon**, and Wresti Indriatmi*** BACKGROUND Syphilis is a multistage disease transmitted primarily through sexual intercourse. Nowadays, the polymerase chain reaction (PCR) test for Treponema pallidum has been widely used and is expected to overcome problems in diagnostic tests for syphilis. The Treponema pallidum PCR is influenced by type of specimens, PCR methods and target genes. This study aimed to assess the use of blood and serum in multiplex nested PCR for Treponema pallidum, targeting the 23S rRNA. METHODSA cross-sectional study was conducted from April 2015 -April 2016. Sampling was carried out consecutively among patients with clinical features of secondary syphilis who came to Sexually Transmitted Disease (STD) clinics in Jakarta. All sera were also tested with Rapid Plasma Reagin (RPR) and Treponema pallidum Hemagglutination Assay (TPHA) assay, which was considered as the gold standard for this study. We determined the sensitivity and specificity of the multiplex nested PCR for Treponema pallidum using blood and serum. RESULTS PCR test was performed on 122 clinical specimens (61 blood and 61 serum).The positive results of PCR test on blood was 22.95% and serum was 6.56%, while the positive results of serology was 68.85%. The sensitivity of Treponema pallidum multiplex nested PCR on blood was 30.95% compared to serum 9.52% (p=0.006). PCR test on blood is able to detect 3.25 times higher than serum. CONCLUSIONThe use of blood has a higher proportion of positives compared to serum in Treponema pallidum multiplex nested PCR using 23S rRNA target gene.

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Molecular detection of Treponema pallidum sp. pallidum in blood samples of VDRL-seroreactive women with lethal pregnancy outcomes: a retrospective observational study in northern Brazil

Cited by 15 publications

References 28 publications

Risk factors for syphilis in women: case-control study

Risk factors for syphilis in women: case-control study

Risk factors and pregnancy outcomes in women with syphilis diagnosed using a molecular approach

Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum

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