Destruição de larvas infectantes de Strongyloides venezuelensis pelos fungos Duddingtonia flagrans, Arthrobotrys robusta e Monacrosporium sinense

Braga, Fábio Ribeiro; Araújo, Juliana Milani; Silva, André Ricardo e; Araújo, Jackson Víctor de; Carvalho, Rogério Oliva; Tavela, Alexandre de Oliveira; Silva, Manoel Eduardo da; Fernandes, Fernanda Mara; Melo, Alan Lane de

doi:10.1590/s0037-86822011000300026

Cited by 11 publications

(8 citation statements)

References 11 publications

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“…The fungal species most commonly found was M. eudermatum. Some species of the Monacrosporium genus have been considered potential agents for control of animal parasitic diseases [24][25][26]. Another species we identified was A. oligospora which is the most intensively studied nematophagous species worldwide and a number of important research works are published every year about its use against parasitic nematodes of either plants [27,28] or animals [29,30].…”

Section: Discussionmentioning

confidence: 99%

“…The yearly average temperature is 21.1 ∘ C, with yearly average precipitation between 800 and 1,500 mm. The highest temperatures (24)(25)(26)(27)(28) ∘ C) occur in April and May. The lowest monthly temperature average (below 15 ∘ C) occurs in December and January [9].…”

Section: Area Of Study For Fungal Isolationmentioning

confidence: 99%

“…A previously described method of sprinkling soil on 2% agar plates for fungal isolation [12] was used. Each sample was sprinkled on the surface of 9 cm diameter water agar plates and incubated at room temperature (18)(19)(20)(21)(22)(23)(24)(25) ∘ C) and 60% humidity. After 2 days, an undetermined number of mixed stages of free-living nematodes (Panagrellus redivivus) were added to the agar surface of each plate to promote nematophagous fungal development.…”

Section: Fungalmentioning

confidence: 99%

“…Faecal material was processed to elaborate faecal cultures [19]. These cultures were incubated for 7 days at room temperature (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28) ∘ C). Each day, they were mixed with a wooden spoon and hydrated with tap water to promote maximum egg hatching and larva production.…”

Section: Haemonchus Contortus (L3) Infective Larvaementioning

confidence: 99%

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Phylogenetic Analysis of Nucleotide Sequences from the ITS Region and Biological Characterization of Nematophagous Fungi from Morelos, Mexico

Castañeda-Ramírez

Mendoza-de-Gives

Aguilar-Marcelino

et al. 2016

Journal of Mycology

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We determined the morphological taxonomy of eighteen nematophagous fungi (NF), as well as their in vitro predatory activity against Haemonchus contortus infective larvae (L3). Fungi were classified into six genera and three species, the most common of which were Monacrosporium eudermatum and Arthrobotrys oligospora. We then sequenced five NF isolates using ITS4 and ITS5 primers. These sequences showed high identity with sequences from the NCBI database (98-99%). In contrast, alignments among the same genera and species demonstrated 83-97% identity. Polymorphisms observed between Arthrobotrys and Monacrosporium appear to be associated with differences in biological function, nonspecific mutations, evolutionary processes, feeding behaviour, predatory activity, and microecosystems.

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Section: Discussionmentioning

confidence: 99%

Section: Area Of Study For Fungal Isolationmentioning

confidence: 99%

Section: Fungalmentioning

confidence: 99%

Section: Haemonchus Contortus (L3) Infective Larvaementioning

confidence: 99%

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Phylogenetic Analysis of Nucleotide Sequences from the ITS Region and Biological Characterization of Nematophagous Fungi from Morelos, Mexico

Castañeda-Ramírez

Mendoza-de-Gives

Aguilar-Marcelino

et al. 2016

Journal of Mycology

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“…).Estudo com fungos nematófagos tem apresentado resultados promissores como potenciais controladores biológicos. As espécies de fungos nematófagos Duddingtonia flagrans, Arthrobotrys robusta e Monacrosporium sinense são identificadas como predadoras de nematoides e têm atividade predatória sobre larvas infectantes de S. venezuelensis(BRAGA et al, 2011).Estudo recente demonstrou também a utilização de probióticos na diminuição da carga parasitaria de S. venezuelensis em camundongos. No qual foi comprovado que houve redução de 33% das fêmeas parasitos no intestino, 21% de eliminação de ovos e também foi observado que a altura das vilosidades no duodeno em animais tratados com probiotico sofrem menos agressão(OLIVEIRA-SEQUEIRA et al, 2014).Os parasitos do gêneroSyphacia pertencem à classe Nematoda, ordenar Ascarida, subordem Oxyurina.…”

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Avaliação parasitológica e sorológica de coinfecção por Syphacia muris e Strongyloides venezuelensis

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Agradeço, primeiramente, a Deus por me proteger e sempre estar ao meu lado, em todos os momentos da minha vida.Aos meus pais Mônica e José Carlos que não mediram esforços para que eu chegasse até esta etapa de minha vida. Obrigado por ter me fornecido está oportunidade. Gostaria de agradecer especialmente minha mãe mesmo à distância, seu cuidado е dedicação foi que deram, em alguns momentos, а esperança para seguir. Aos meus irmãos Wanderlei, Diego e Karla por serem acima de tudo, meus amigos. A minha família principalmente meus avós paternos José Eduardo e Sebastiana e aos maternos Otacílio e Ivete, por serem exemplos de pessoas humildes e honestas. Aos meus sobrinhos Camila e Vinícius. A todos os meus tios (as) e primos (as). Devo tudo o que sou hoje, a minha família. Muito obrigado a todos. À Profa. Dra. Julia Maria Costa Cruz, pela orientação, oportunidade e dedicação, mostrando-se sempre disposta a resolver os problemas que surgiram neste período de dois anos. Quero expressar o meu reconhecimento e admiração pela sua competência profissional, por ser uma profissional extremamente qualificada e pela forma humana que conduziu minha orientação. Muito Obrigado por tudo. A toda equipe do laboratório Daniela, Gabriela, Lucas, Renata, Camila, Nágilla e Henrique que se tornaram grandes companheiros e amigos. Agradeço em especial meu colega Edson por todo apoio e cumplicidade e que durante esse período acabou se transformando em um grande amigo. As minhas colegas de mestrado Elivaine, Layane, Leilaine, Melina, Pâmela e Letícia. Agradeço a todas pelo convívio e troca de experiências. Gostaria de agradecer especialmente aos colegas Murilo e Eliezer que se tornaram grandes amigos no decorrer destes dois anos. Aos funcionários do laboratório de Parasitologia Juliana, Scheila e Elaine. Agradeço em especialmente a Maria do Rosário e ao Marcelo pela paciência e disponibilidade nunca negada.As pessoas que ao longo do tempo, desde a infância viram meu crescimento enquanto pessoa, enquanto amigo, enquanto estudante e em busca de novos sonhos e horizontes,

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Viability of Strongyloides venezuelensis eggs and larvae in vermiculite containing the fungus Duddingtonia flagrans

et al. 2017

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Strongyloidiasis is the most clinically important disease among the infections caused by geohelminths, seeing that this parasite can cause autoinfection. The use of nematophagous fungi like Duddingtonia flagrans, that have predation action on eggs and infecciososas forms of helminths, emerges as an alternative method for environmental control. For this reason, analyzing the viability of larvae and eggs of Strongyloides venezuelensis and the action of Duddingtonia flagrans AC001 in vermiculite, as well as the action of the nematophagous fungi in different growth stages, is important to elaborate and define the best culture conditions that favor the activity of the fungus. Two different growth conditions were applied: both eggs and AC001 fungi were added at the same time to the vermiculite (assay A) and the addition of eggs after the growth of the AC001 fungi in the vermiculite (assay B). To recover the L larvae, the Baermann-Moraes method was applied, followed by the counting of L dead and alive. At last, it was observed that the vermiculite enriched with organic material is an adequate culture medium not only for the growth of the S. venezuelensis but also for the growth of the D. flagrans fungus, being therefore, a satisfactory culture medium for tests of viability and predatory action of this fungus. It was also observed that the activity of the AC001 fungus is greater when it is growing concomitantly with the eggs, in other words, when it is in the adaptation phase.

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Destruição de larvas infectantes de Strongyloides venezuelensis pelos fungos Duddingtonia flagrans, Arthrobotrys robusta e Monacrosporium sinense

Cited by 11 publications

References 11 publications

Phylogenetic Analysis of Nucleotide Sequences from the ITS Region and Biological Characterization of Nematophagous Fungi from Morelos, Mexico

Phylogenetic Analysis of Nucleotide Sequences from the ITS Region and Biological Characterization of Nematophagous Fungi from Morelos, Mexico

Avaliação parasitológica e sorológica de coinfecção por Syphacia muris e Strongyloides venezuelensis

Viability of Strongyloides venezuelensis eggs and larvae in vermiculite containing the fungus Duddingtonia flagrans

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