Caracterização de cepas de referência de Leptospira sp utilizando a técnica de pulsed field gel electrophoresis

Machry, Lívia; Ribeiro, Rachel Leite; Vital-Brazil, Juliana Magalhães; Balassiano, Ilana Teruszkin; Oliveira, Ivi Cristina Menezes de; Avelar, Kátia Eliane Santos; Pereira, Martha María

doi:10.1590/s0037-86822010000200012

Cited by 8 publications

(6 citation statements)

References 17 publications

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“…Serovar identification by pulsed field gel electrophoresis - Pulsed field gel electrophoresis (PFGE) was performed using the NotI restriction enzyme to generate fingerprint patterns, as previously described by (Machry et al 2010). Pulse marker 0.5-1000 kb (SIGMA) was used as a size standard.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity of Leptospira in northwestern Colombia: first report of Leptospira santarosai as a recognised leptospirosis agent

Sanchez

López

Pereira

et al. 2016

Mem. Inst. Oswaldo Cruz

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The region of Antioquia in northeastern Colombia has the highest number of reported leptospirosis cases in the country. It also shows high seroprevalence indexes in the general population and socio-environmental conditions favourable for the transmission of the disease between humans and animals. In this study, 25 Leptospira isolates from Colombia’s Antioquia department were identified to the species level as L. santarosai (12), L. interrogans (9) and L. meyeri (4) using phylogenetic analysis of the Amidohydrolase gene. Typing at the serovar level was performed using multilocus sequence typing (MLST) and monoclonal antibodies. The serovars Canalzonae, Babudieri, Alice, Beye, and Copenhageni have been identified as causing human or animal infections in Antioquia, Colombia. The four environmental isolates were not identified to the serovar level. L. santarosai serovar Canalzonae and Alice were identified as new etiologic agents of human leptospirosis in Antioquia, Colombia. This paper reports species and serovars that were previously unknown in the region.

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Section: Methodsmentioning

confidence: 99%

Genetic diversity of Leptospira in northwestern Colombia: first report of Leptospira santarosai as a recognised leptospirosis agent

Sanchez

López

Pereira

et al. 2016

Mem. Inst. Oswaldo Cruz

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“…Leptospirosis is an acute, infectious, and systemic disease caused by a pathogenic bacterium of the Leptospiraceae family, Leptospira gender known as L. interrogans; more than 200 serovars have been described, with distribution into 19 serogroups (1) .…”

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confidence: 99%

Seroprevalence of and risk factors for leptospirosis in the City of Manaus, State of Amazonas, Brazil

Silva¹,

Lima²,

Fernandes³

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction:Leptospirosis is caused by a bacterium of the genus Leptospira. This study aimed at investigating the seroprevalence of and risk factors for leptospirosis in humans in Manaus, State of Amazonas. Methods: Interviews were performed, and 1,000 blood serum samples were examined using a microscopic agglutination test. Results: Forty-three cases were positive; there were 10 serotypes, with coagglutination in 8 cases. The most frequently occurring serotypes were Icterohaemorrhagiae (20.7%), Cynopteri (20.7%), Australis (18.8%), and Copenhageni (16.9%), and the Midwest (54.7%) and South (23.8%) had the most cases; these areas lack basic sanitation. Conclusions: Disease occurrence might be reduced through improved basic infrastructural conditions.

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“…sirvió como una biblioteca de búsqueda para la comparación e identificación de serovares de los aislamientos estudiados. Se comparó con los resultados obtenidos por Galloway y Levett y el IOC-Fiocruz para las cepas referenciales (12,21) , y por Ganoza et al y Matthias et al para algunos de nuestros aislamientos (7,15) .…”

Section: Digestión De Restricciónunclassified

Diversidad genética de aislamientos peruanos de Leptospira spp. mediante electroforesis en gel de campo pulsado

Rivera¹,

Ticlla

Balda

et al. 2014

Rev Peru Med Exp Salud Publica

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Caracterização de cepas de referência de Leptospira sp utilizando a técnica de pulsed field gel electrophoresis

Cited by 8 publications

References 17 publications

Genetic diversity of Leptospira in northwestern Colombia: first report of Leptospira santarosai as a recognised leptospirosis agent

Genetic diversity of Leptospira in northwestern Colombia: first report of Leptospira santarosai as a recognised leptospirosis agent

Seroprevalence of and risk factors for leptospirosis in the City of Manaus, State of Amazonas, Brazil

Diversidad genética de aislamientos peruanos de Leptospira spp. mediante electroforesis en gel de campo pulsado

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