Primeiro workshop interno dos serviços de referência do Centro de Pesquisas Aggeu Magalhães da Fundação Oswaldo Cruz

Rocha, Abraham; Barbosa, Constança Simões; Brandão-Filho, Sinval Pinto; Oliveira, Cláudia Maria Fontes de; Almeida, Alzira Maria Paiva de; Gomes, Yara de Miranda

doi:10.1590/s0037-86822009000200030

Cited by 4 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomes were sequenced using the Illumina HiSeq 2000 platform (Illumina, Inc., San Diego, CA, USA) using standard protocols for the TruSeq SBS Kit v3-HS (Illumina, Code FC-401-3001) [3]. The strains belong to the Y. pestis cultures collection Fiocruz/CYP (http://cyp.fiocruz.br/accessed on 4 April 2019) of the Aggeu Magalhães Institute (IAM/Fiocruz-PE) [17]. The collection began as an initiative of the Brazilian government and the World Health Organization (WHO) in which a broad research program was developed for plague control in the Araripe plateau between 1966 and 1974.…”

Section: Biological Datamentioning

confidence: 99%

Genomic Analysis of Yersinia pestis Strains from Brazil: Search for Virulence Factors and Association with Epidemiological Data

et al. 2023

Self Cite

View full text Add to dashboard Cite

Yersinia pestis, the etiological agent of the plague, is considered a genetically homogeneous species. Brazil is currently in a period of epidemiological silence but plague antibodies are still detected in sentinel animals, suggesting disease activity in the sylvatic cycle. The present study deployed an in silico approach to analyze virulence factors among 407 Brazilian genomes of Y. pestis belonging to the Fiocruz Collection (1966–1997). The pangenome analysis associated several known virulence factors of Y. pestis in clades according to the presence or absence of genes. Four main strain clades (C, E, G, and H) exhibited the absence of various virulence genes. Notably, clade G displayed the highest number of absent genes, while clade E showed a significant absence of genes related to the T6SS secretion system and clade H predominantly demonstrated the absence of plasmid-related genes. These results suggest attenuation of virulence in these strains over time. The cgMLST analysis associated genomic and epidemiological data highlighting evolutionary patterns related to the isolation years and outbreaks of Y. pestis in Brazil. Thus, the results contribute to the understanding of the genetic diversity and virulence within Y. pestis and the potential for utilizing genomic data in epidemiological investigations.

show abstract

Section: Biological Datamentioning

confidence: 99%

Genomic Analysis of Yersinia pestis Strains from Brazil: Search for Virulence Factors and Association with Epidemiological Data

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…In recognition of its importance, this culture collection was institutionalized in 2007 and named ‘Collection of Yersinia pestis ’ with the acronym Fiocruz‐CYP and is currently maintained by the National Plague Reference Service (SRP) of FIOCRUZ‐PE (Rocha et al . ,b).…”

Section: Introductionmentioning

confidence: 99%

Viability of Yersinia pestis subcultures in agar stabs

Leal

Sobreira

Araujo

et al. 2015

Lett Appl Microbiol

View full text Add to dashboard Cite

We report the ability of Yersinia pestis to survive up to 47 years in agar stabs, in rubber-stoppered tubes, under refrigeration (+4 to +10°C), although overall subculture recovery rates were poor and inversely related to the length of time stored. Genetic characterization of virulence gene presence among these subcultures was suggestive of significant variation in the genomic stability of Y. pestis subcultures stored under these conditions. This variation, together with all of the inherent temporal, geographic and other genetic variation represented by all of the recoverable strains in the historical 'Collection of Yersinia pestis' (Fiocruz-CYP) maintained by the SRP of FIOCRUZ-PE in Brazil was preserved in new frozen culture stocks stored at -70°C as a result of this study. These frozen culture stocks represent a valuable resource for future comparative studies of Y. pestis.

show abstract

Perfil Epidemiológico De Pacientes Atendidos No Ambulatório Do Serviço De Referência Nacional Em Filarioses Do Centro De Pesquisas Aggeu Magalhães, Recife-Pe

et al. 2015

View full text Add to dashboard Cite

Este trabalho descreve o perfil epidemiológico dos pacientes atendidos no ambulatório do Serviço de Referência Nacional em Filarioses (SRNF), onde são desenvolvidas atividades clínicas, epidemiológicas e laboratoriais relacionadas à pesquisa e assistência aos pacientes acometidos por filarioses. As informações foram obtidas de 1.109 prontuários de pacientes atendidos no período de seis anos. Do total de prontuários analisados, foi observado que 62,8% dos indivíduos atendidos eram do gênero masculino e que 85,4% eram residentes na Região Metropolitana do Recife (RMR). A frequência de indivíduos encaminhados ao SRNF que se apresentavam microfilarêmicos e/ou antígenos positivos para filariose bancroftiana foi de 55%. Tal resultado foi considerado baixo uma vez que esses indivíduos apresentavam forte indicativo clínico ou epidemiológico para infecção filarial. Esta informação evidencia a necessidade de um esforço maior no estabelecimento de parâmetros que determinem o fluxo de encaminhamento dos pacientes ao SRNF, de modo que se evite demanda desnecessária e sejam otimizados os recursos. DESCRITORES: Filariose; assistência à saúde; perfil de saúde; acesso aos serviços de saúde.

show abstract

Primeiro workshop interno dos serviços de referência do Centro de Pesquisas Aggeu Magalhães da Fundação Oswaldo Cruz

Cited by 4 publications

References 17 publications

Genomic Analysis of Yersinia pestis Strains from Brazil: Search for Virulence Factors and Association with Epidemiological Data

Genomic Analysis of Yersinia pestis Strains from Brazil: Search for Virulence Factors and Association with Epidemiological Data

Viability of Yersinia pestis subcultures in agar stabs

Perfil Epidemiológico De Pacientes Atendidos No Ambulatório Do Serviço De Referência Nacional Em Filarioses Do Centro De Pesquisas Aggeu Magalhães, Recife-Pe

Contact Info

Product

Resources

About