2015
DOI: 10.1111/lam.12519
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Viability of Yersinia pestis subcultures in agar stabs

Abstract: We report the ability of Yersinia pestis to survive up to 47 years in agar stabs, in rubber-stoppered tubes, under refrigeration (+4 to +10°C), although overall subculture recovery rates were poor and inversely related to the length of time stored. Genetic characterization of virulence gene presence among these subcultures was suggestive of significant variation in the genomic stability of Y. pestis subcultures stored under these conditions. This variation, together with all of the inherent temporal, geographi… Show more

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Cited by 5 publications
(4 citation statements)
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References 21 publications
(35 reference statements)
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“…In contrast, routine Y . pestis culture collection did not start until the mid-1960s [6, 12]. As such, the available strain diversity is limited, with the majority of strains originating from the most active northeastern plague foci, including the six foci represented here.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…In contrast, routine Y . pestis culture collection did not start until the mid-1960s [6, 12]. As such, the available strain diversity is limited, with the majority of strains originating from the most active northeastern plague foci, including the six foci represented here.…”
Section: Resultsmentioning
confidence: 99%
“…pestis strains belonging to the Y . pestis culture collection (FIOCRUZ–CYP) of the SRP/IAM [12] using the QIAGEN DNeasy Blood and Tissue Kit (QIAGEN, Inc., Germantown, MD). The strains were isolated from 1966 to 1997 from six plague foci in Brazil and include strains isolated from humans, rodents, and fleas (S1 Table).…”
Section: Methodsmentioning
confidence: 99%
“…The genes analyzed using the VFDB returned several virulence factors, including important well-known genes in Y. pestis , such as those involved in biofilm formation, iron acquisition, and cell adhesion genes [ 35 , 36 , 37 ]. The gene clusters returned by Scoary in clades C, E, G, and H are characterized by the absence of virulence genes; however, it is necessary to consider the collection period of these strains (some from the 1960s), the prolonged laboratory handling and storage process in agar stabs, in rubber-stoppered tubes, and under refrigeration at 4 °C [ 16 ].…”
Section: Discussionmentioning
confidence: 99%
“…The present study used 411 genomes of Y. pestis belonging to the National Reference Service in Plague (Serviço de Referência em Peste—SRP). The strains were isolated from rodent, flea, and human cases from epidemic and endemic periods during surveillance activities since 1966 and were maintained in plain agar stubs, in rubber-stoppered tubes, under refrigeration at 4 °C [ 16 ]. These genomes are deposited in the NCBI database under BioProject: PRJNA421720.…”
Section: Methodsmentioning
confidence: 99%