Infecções oportunistas por leveduras e perfil enzimático dos agentes etiológicos

Macêdo, Danielle Patrícia Cerqueira; Farias, Aline Mary de Almeida; Neto, Reginaldo Gonçalves de Lima; Silva, Vanessa Karina Alves da; Leal, André Ferraz Goiana; Neves, Rejane Pereira

doi:10.1590/s0037-86822009000200019

Cited by 7 publications

(9 citation statements)

References 24 publications

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“…Similarly, the present study showed a high prevalence of Candida non-albicans species in the group of children with AIDS -a condition equally favorable to the emergence of opportunistic infections -and in the groups of children without AIDS. The most frequent Candida non-albicans species were C. parapsilosis, C. famata, and C. tropicalis, as reported by Macedo et al 17 . The main clinically relevant Candida species are Candida albicans, C. parapsilosis, and C. tropicalis.…”

supporting

confidence: 70%

“…The main clinically relevant Candida species are Candida albicans, C. parapsilosis, and C. tropicalis. However, an increasing number of cases of superficial and invasive diseases caused by emerging Candida species (species that have appeared recently in a population or whose incidence and geographic range has increased rapidly) have been described and include C. dubliniensis, C. kefyr, C. famata, C. utilis, and C. norvegensis, among others [17][18] . In the present study, the Candida species considered the most important clinically and that are emerging were isolated from the oral cavity of children exposed and non-exposed to HIV and of children with AIDS.…”

mentioning

confidence: 99%

“…The same species were also isolated from HIV-positive and HIV-negative children by Cerqueira et al 16 . The correct identification of the yeast species has great clinical and epidemiological relevance and indicates the species that are resistant to conventionally antifungal agents used in clinical practice [16][17][18][19] . Similar to the results of this study, Domaneschi et al 4 and Cerqueira et al 16 identified Candida tropicalis, C. kefyr, C. krusei, and C. glabrata isolates from the oral cavity of HIV-positive children and children with AIDS treated at a hospital in São Paulo.…”

mentioning

confidence: 99%

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Prevalence of Candida yeasts in oral samples from children with AIDS and children exposed and not exposed to HIV served by SUS in the state of Bahia, Brazil

Silva

Viana

Araújo

et al. 2015

RGO, Rev. Gaúch. Odontol.

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ObjectiveThis study aimed to determine the prevalence of Candida albicans and non-albicans yeast species isolated from oral samples of children with AIDS and of children exposed and not exposed to HIVduring pregnancy and served by the public health system in a county located in the interior of the state of Bahia, Brazil. Methods Saliva samples from 50 children aged between 2 and 12 years treated by SUS (group I = control group, group II = group exposed to HIV without seroconversion, and group III = AIDS carriers) were collected, seeded in Sabouraud dextrose agar with chloramphenicol, and fungal cultures were grown at 35 ± 2° C for 24 hours. Three isolated colonies were randomly selected from each individual plaque for identification using the API20 AUX Biomerieux® method. ResultsThe most frequent fungal species in samples from children not exposed to HIV, exposed to HIV, and AIDS carriers was Candida albicans (48.80%, 25.64%, and 58.13%, respectively). Species of Candida non-albicans were also isolated from all groups studied. ConclusionsDespite the higher prevalence of Candida albicans in these groups, non-albicans species represented a significant percentage of Candida isolates.

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supporting

confidence: 70%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Prevalence of Candida yeasts in oral samples from children with AIDS and children exposed and not exposed to HIV served by SUS in the state of Bahia, Brazil

Silva

Viana

Araújo

et al. 2015

RGO, Rev. Gaúch. Odontol.

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“…The maximum temperature of growth for each isolate was determined according Barnett et al (2000) and De Hoog et al (2000). The isolates which presented ambiguous identification were further analysed by VITEK-2 XL (bioMerieux, Brazil) according to previous reports (Macedo et al , 2009). …”

Section: Methodsmentioning

confidence: 99%

Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection

Lima-Neto¹,

Santos²,

Lima³

et al. 2014

Braz. J. Microbiol.

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Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical isolates were identified by morphological and biochemical analyses. Identifications were also performed by the new proteomic approach based on MALDI-TOF MS. Results demonstrated 15% discordance when compared with morphological and biochemical analyses. Discordant isolates were analysed by ITS sequencing, which confirmed the MALDI-TOF MS identifications and these strains were renamed in the culture collection catalogue. In conclusion, proteomic profiles by MALDI-TOF MS represents a rapid and reliable method for identifying clinical Candida species preserved in culture collections and may present clear benefits when compared with the performance of existing daily routine methods applied at health centres and hospitals.

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“…Entretanto, é capaz de causar infecção se o equilíbrio com o hospedeiro é quebrado como nos casos em que se observa baixa imunidade nos pacientes; sob manifestações clínicas e fatores de risco relacionados à neutropenia, diabetes mellitus, tuberculose pulmonar, lúpus eritematoso sistêmico, síndrome da imunodeficiência adquirida (SIDA ou AIDS), uso de drogas imunossupressoras, mucosite induzida por quimioterapia, altas doses de irradiação e antibioticoterapia (MACEDO et al, 2009;MARR, 2005). Pacientes transplantados apresentam alto risco de desenvolver infecções sanguíneas fúngicas.…”

Section: Introductionunclassified

Ocorrência das espécies de leveduras isoladas de sangue e cateter de pacientes internados em Hospital Público Infantil de São Paulo (período 2007 a 2010).

Oliveira¹

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Neste trabalho, investigamos a ocorrência de espécies de leveduras isoladas de sangue e cateter de pacientes internados em um hospital público infantil no período de 2007 a 2010. Pesquisamos a presença de C. dubliniensis pelos métodos fenotípicos; investigamos as espécies dentro do complexo Candida parapsilosis por meio da técnica do seqüenciamento; e estudamos as espécies do complexo Candida parapsilosis quanto a produção de biofilme. Também investigamos os fatores relacionados a virulência: proteinase, fosfolipase, hemolisina e hialuronidase; e estudamos o perfil de sensibilidade das amostras frente aos antifúngicos a anfotericina B, fluconazol e caspofungina pelo método "Etest ® ". Observamos que, dentre os anos em estudo, C. albicans foi a levedura mais isolada com 36.45%, seguida de C. tropicalis 23.36%, C. parapsilosis sensu stricto 21.49%, Pichia anômala 5.61%, C. guilliermondii 4.67%, C. krusei 2.80%; C. orthopsilosis 1.87%, C. glabrata 1.87%, C. metapsilosis 0.94%, C. pararugosa 0.94%. Na pesquisa de C. dubliniensis, todas as amostras cresceram a 45°C, bem como, todas cresceram no "caldo Sabouraud dextrose" hipertônico, sendo, desta forma, identificadas como C. albicans. No seqüenciamento das amostras do complexo Candida parapsilosis, C. parapsilosis sensu estricto foi a espécie mais prevalente, representando 88,46% dos isolados, C. orthopsilosis e C. metapsilosis apresentaram apenas 7,69% e 3,85% respectivamente. Na produção de biofilme por amostras do complexo Candida parapsilosis, oito amostras de C. parapsilosis sensu estricto produziram biofilme, e apenas uma amostra de C. orthopsilosis foi produtora, enquanto que C. metapsilosis não produziu biofilme. Em relação aos fatores de virulência, as espécies não-albicans apresentaram maior atividade de proteinase e hialuronidase do que as C. albicans. No estudo das exoenzimas fosfolipase e hemolisina, C. albicans foi mais produtora, quando comparada com as espécies não-albicans. Das 107 amostras em estudo, frente ao antifúngico anfotericina B todas as amostras foram sensíveis. Com relação ao antifúngico fluconazol, 11 (10.3%) amostras demonstraram sensibilidade dose-dependente (SDD), e quatro (3.74%) foram resistentes. Em relação a caspofungina, duas amostras de C. guilliermondii foram resistentes, enquanto que as amostras de C. parapsilosis produziram os mais altos valores de CIM 50 do que as demais espécies. Dos antifúngicos em estudo, todos parecem ser mais ativos contra C. albicans que espécies não-albicans.

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Infecções oportunistas por leveduras e perfil enzimático dos agentes etiológicos

Cited by 7 publications

References 24 publications

Prevalence of Candida yeasts in oral samples from children with AIDS and children exposed and not exposed to HIV served by SUS in the state of Bahia, Brazil

Prevalence of Candida yeasts in oral samples from children with AIDS and children exposed and not exposed to HIV served by SUS in the state of Bahia, Brazil

Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection

Ocorrência das espécies de leveduras isoladas de sangue e cateter de pacientes internados em Hospital Público Infantil de São Paulo (período 2007 a 2010).

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