Atividade enzimática, produção de slime e sensibilidade a antifúngicos de Candida sp

Silva, Jaqueline Otero; Ferreira, Joseane Cristina; Candido, Renato

doi:10.1590/s0037-86822007000300023

Cited by 6 publications

(8 citation statements)

References 7 publications

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“…It is important to note that 21.5% of the Candida non-albicans samples isolated from the environment showed proteinase activity. Other studies demonstrate a larger incidence of proteinase production by Candida non-albicans species 25,29,59,60 .…”

Section: Discussionmentioning

confidence: 93%

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Candida spp. isolated from inpatients, the environment, and health practitioners in the pediatric unit at the Universitary Hospital of the Jundiaí Medical College, state of São Paulo, Brazil

Storti

Pasquale

Scomparim

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: This study aimed to isolate and identify Candida spp. from the environment, health practitioners, and patients with the presumptive diagnosis of candidiasis in the Pediatric Unit at the Universitary Hospital of the Jundiaí Medical College, to verify the production of enzymes regarded as virulence factors, and to determine how susceptible the isolated samples from patients with candidiasis are to antifungal agents. METHODS: Between March and November of 2008 a total of 283 samples were taken randomly from the environment and from the hands of health staff, and samples of all the suspected cases of Candida spp. hospital-acquired infection were collected and selected by the Infection Control Committee. The material was processed and the yeast genus Candida was isolated and identified by physiological, microscopic, and macroscopic attributes. RESULTS: The incidence of Candida spp. in the environment and employees was 19.2%. The most frequent species were C. parapsilosis and C. tropicalis among the workers, C. guilliermondii and C. tropicalis in the air, C. lusitanae on the contact surfaces, and C. tropicalis and C. guilliermondii in the climate control equipment. The college hospital had 320 admissions, of which 13 (4%) presented Candida spp. infections; three of them died, two being victims of a C. tropicalis infection and the remaining one of C. albicans. All the Candida spp. in the isolates evidenced sensitivity to amphotericin B, nystatin, and fluconazole. CONCLUSIONS: The increase in the rate of hospital-acquired infections caused by Candida spp. indicates the need to take larger measures regarding recurrent control of the environment.

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Section: Discussionmentioning

confidence: 93%

“…A number of works have implied that the proteinase and phospholipase enzymes might be virulence factors of Candida spp [59][60][61][62] .…”

Section: Discussionmentioning

confidence: 99%

Candida spp. isolated from inpatients, the environment, and health practitioners in the pediatric unit at the Universitary Hospital of the Jundiaí Medical College, state of São Paulo, Brazil

Storti

Pasquale

Scomparim

et al. 2012

Rev. Soc. Bras. Med. Trop.

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“…De acordo com Silva e cols 30 , esta metodologia pode ser utilizada na caracterização fenotípica de leveduras e, portanto, servir como um marcador epidemiológico de infecções por Candida, especialmente em ambiente hospitalar que predispõe os imunocomprometidos a leveduroses graves e de repetição devido aos procedimentos invasivos a que estes pacientes estão expostos 18 .…”

Section: Discussionunclassified

“…A habilidade de leveduras de interesse médico em secretar proteases tem sido associada a fatores de patogenicidade 30 .…”

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Infecções oportunistas por leveduras e perfil enzimático dos agentes etiológicos

Macêdo

Farias

Neto

et al. 2009

Rev. Soc. Bras. Med. Trop.

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Infecções por leveduras são freqüentes em imunocomprometidos, contudo espécies emergentes têm alterado o perfil epidemiológico. A habilidade de secretar proteases tem sido associada à patogenicidade do gênero Candida. Esta pesquisa teve como objetivos diagnosticar leveduroses em pacientes imunocomprometidos e avaliar a virulência dos agentes etiológicos baseado em teste de secreção de protease utilizando soro de albumina bovina como substrato. Do total de 104 pacientes estudados, 19 apresentaram episódios de leveduroses. O trato respiratório (63,2%), seguido pelo trato urinário (10,5%) foram os locais mais comuns de infecção. Candida albicans, Candida parapsilosis, Candida tropicalis e espécies emergentes como Candida krusei e Candida guilliermondii foram isoladas. Cinco isolados de Candida parapsilosis e um de Candida albicans e Candida guilliermondii exibiram alta atividade enzimática. Concluímos que a caracterização enzimática de isolados de Candida pode ser um útil marcador prognóstico, especialmente em imunocomprometidos, uma vez que leveduroses nestes pacientes são geralmente graves.

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“…This finding differs from other authors and corroborates the results of other studies in which phospholipase activity was detected in C. albicans, C. parapsilosis, C. tropicalis, and C. guilliermondii (35,36). Enzymatic activity has been detected in yeasts isolated from biological samples from different anatomical origins (anus, vagina, and mouth) in 17 patients (37,38). The detection of phospholipase and proteinase enzymes on solid media has been described, especially in C. albicans samples.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic characteristics of yeasts isolated from vaginal secretion preserved under mineral oil

Gomes¹,

Souza-Motta²,

Lima³

et al. 2011

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:In order to evaluate the pathogenicity of yeasts isolated from vaginal secretion of pregnant and non-pregnant women -stored in mineral oil at the URM Mycology Collection, Department of Mycology, Federal University of Pernambuco -30 samples belonging to the genera Candida, Rhodotorula, Trichosporon, and Kloeckera, were studied regarding their pathogenic characteristics, ability to grow at room temperature (28°C ± 1°C), 37°C, and 42°C for 72 hours, and production of both phospholipase and proteinase. Results showed that all 30 isolates (100%) were able to grow at room temperature and 37°C, and that 17 samples (57%) were able to grow at 42°C. Evaluation of enzymatic activity showed protease activity in only two isolates (7%), namely C. maritima and C. obtusa. Phospholipase activity was detected in 20 isolates (67%) using soy lecithin as substrate at different temperatures. The characterization of yeasts isolated from vaginal secretion and determination of their enzymatic activity may contribute to understanding the epidemiology of vulvovaginitis and assist in the treatment of patients.

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Atividade enzimática, produção de slime e sensibilidade a antifúngicos de Candida sp

Cited by 6 publications

References 7 publications

Candida spp. isolated from inpatients, the environment, and health practitioners in the pediatric unit at the Universitary Hospital of the Jundiaí Medical College, state of São Paulo, Brazil

Candida spp. isolated from inpatients, the environment, and health practitioners in the pediatric unit at the Universitary Hospital of the Jundiaí Medical College, state of São Paulo, Brazil

Infecções oportunistas por leveduras e perfil enzimático dos agentes etiológicos

Pathogenic characteristics of yeasts isolated from vaginal secretion preserved under mineral oil

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