Isolamento e identificação de amebas de vida livre potencialmente patogênicas em amostras de ambientes de hospital público da cidade de Porto Alegre, RS

Carlesso, Ana Maris; Simonetti, Amauri Braga; Artuso, Georgia Lazzari; Rott, Marilise Brittes

doi:10.1590/s0037-86822007000300013

Cited by 21 publications

(5 citation statements)

References 27 publications

(2 reference statements)

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“…The present results showed that 50% of the samples (fresh water and dust) collected from a hospital environment in the Southern Brazil harbored potentially pathogenic species of Acanthamoeba (A. castellanii and A. polyphaga), representing a risk for patients and visitors. These data corroborated those of previous works developed in different Brazilian hospitals (Carlesso et al 2007;Texeira et al 2009;Carlesso et al 2010;Costa et al 2010), and emphasized the need for increasing the public awareness and hospital disinfection measures in order to reduce the incidence of Acanthamoeba infections (Carvalho et al 2009).…”

Section: Discussionsupporting

confidence: 90%

“…Nevertheless as observed in Table 1, five out of ten samples (50%) collected at community bathrooms of a public hospital (two fresh water and three dust samples) exhibited the presence of Acanthamoeba. Compared with other Brazilian studies, the prevalence of Acanthamoeba was lower than that obtained by the analysis of dust samples from a university hospital in Curitiba, PR (Costa et al 2010), but higher than that reported in the hospitals of Porto Alegre, RS (Carlesso et al 2007(Carlesso et al , 2010 and Santos, SP (Teixeira et al 2009). Considering the ubiquitous nature of Acanthamoeba, and the large number of factors that influenced their presence in a hospital environment, it was not surprising to obtain high but variable incidence of these protozoa.…”

Section: Discussioncontrasting

confidence: 56%

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A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples

Zanella

Costa

Zacaria

et al. 2012

Braz. arch. biol. technol.

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Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.

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Section: Discussionsupporting

confidence: 90%

Section: Discussioncontrasting

confidence: 56%

A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples

Zanella

Costa

Zacaria

et al. 2012

Braz. arch. biol. technol.

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“…Its resistant form tolerates adverse environmental conditions, allowing it to survive in the various physical and chemical treatments, and arousing the interest of many researchers in the study of this amphizoic protozoan. Carlesso et al (2007) isolated and characterized amoebae of the genus Acanthamoeba and Naegleria in biofilms and dust, from a hospital in southern Brazil. In this study, the most prevalent genus found was Acanthamoeba.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

Aguiar

Silveira

Winck

et al. 2013

Acta Parasitologica

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The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies.

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“…After sedimentation for 5 minutes, 100 μL of each sample were spread over a plate of non-nutrient agar (PAS + 1.5% agar) containing a lawn of heat-killed Escherichia coli OP50. The plates were incubated at 25°C for 10–14 days and observed daily by light microscopy for the presence of amoeba cysts or trophozoites [15, 16]. Agar sections containing cysts or trophozoites were cut and transferred to new plates to enrich the cultures.…”

Section: Methodsmentioning

confidence: 99%

A hidden battle in the dirt: Soil amoebae interactions with Paracoccidioides spp

et al. 2019

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Paracoccidioides spp. are thermodimorphic fungi that cause a neglected tropical disease (paracoccidioidomycosis) that is endemic to Latin America. These fungi inhabit the soil, where they live as saprophytes with no need for a mammalian host to complete their life cycle. Despite this, they developed sophisticated virulence attributes allowing them not only to survive in host tissues but also to cause disease. A hypothesis for selective pressures driving the emergence or maintenance of virulence of soil fungi is their interaction with soil predators such as amoebae and helminths. We evaluated the presence of environmental amoeboid predators in soil from armadillo burrows where Paracoccidioides had been previously detected and tested if the interaction of Paracoccidioides with amoebae selects for fungi with increased virulence. Nematodes, ciliates, and amoebae–all potential predators of fungi–grew in cultures from soil samples. Microscopical observation and ITS sequencing identified the amoebae as Acanthamoeba spp, Allovahlkampfia spelaea, and Vermamoeba vermiformis. These three amoebae efficiently ingested, killed and digested Paracoccidioides spp. yeast cells, as did laboratory adapted axenic Acanthamoeba castellanii. Sequential co-cultivation of Paracoccidioides with A. castellanii selected for phenotypical traits related to the survival of the fungus within a natural predator as well as in murine macrophages and in vivo (Galleria mellonella and mice). These changes in virulence were linked to the accumulation of cell wall alpha-glucans, polysaccharides that mask recognition of fungal molecular patterns by host pattern recognition receptors. Altogether, our results indicate that Paracoccidioides inhabits a complex environment with multiple amoeboid predators that can exert selective pressure to guide the evolution of virulence traits.

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Isolamento e identificação de amebas de vida livre potencialmente patogênicas em amostras de ambientes de hospital público da cidade de Porto Alegre, RS

Abstract: RESUMO

Cited by 21 publications

References 27 publications

A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples

A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

A hidden battle in the dirt: Soil amoebae interactions with Paracoccidioides spp

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