Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples

Abstract

ABSTRACT

Evaluation of two Taenia solium cysticercal antigenic preparations (vesicular fluid and a glycoprotein fraction with affinity for lentil lectin) for the immunodiagnosis of neurocysticercosis by enzyme-linked immunosorbent assay (ELISA)

“…VF was obtained as previously described 17 . Briefly, the parasites were ruptured individually in petri dishes using two needles and the VF released was collected with a Pasteur pipette and transferred to centrifuges tubes.…”

“…The WEC was obtained as previously described 15,17 , with few modifications. Briefly, parasites maintained at -80 o C were thawed and resuspended in sterile 0.15 M phosphate-buffered saline (PBS), pH 7.2 (1 volume of parasites to 3 volumes of PBS containing PMSF and leupeptin at the concentrations indicated above) and the material then homogenized in an ice-water bath using a Polytron homogenizer equipped with a PT-20 ST probe (three 30 s pulses at speed 3, with 30 s intervals between pulses).…”

“…Crude extracts of whole T. solium or T. crassiceps cysticerci, crude extracts from particular components of the parasites (scolex, membrane or vesicular fluid), excretory-secretory products, purified antigens from crude extracts, recombinant proteins and synthetic peptides have been used in immunological tests to detect specific antibodies [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] . Currently, the most reliable immunological test available for the immunodiagnosis of neurocysticercosis is an enzyme-linked immunoelectrotransfer blot (EITB) assay based on glycoprotein antigens prepared by lentil lectin affinity chromatography from an extract of intact cysts 6,16 .…”

“…However, enzyme-linked immunosorbent assays (ELISAs) are still extensively used to detect anti-cysticercal antibodies because of their simplicity and lower cost compared to EITB. Several studies have shown that ELISAs using vesicular fluid from T. solium and T. crassiceps have good diagnostic performance in terms of sensitivity and specificity 5,7,11,15,17 .…”

Evaluation of an enzyme immunoassay for clinical diagnosis of neurocysticercosis in symptomatic patients

“…Reviewing the literature, it appears that the ELISA method has shown different sensitivity and specificity scores [17][18][19][20][21][22][23][24][25][26][27][28] . This study determined a sensitivity of 31.8% and specificity of 100% for the ELISA method in CSF in relation to diagnosing neurocysticercosis, which implies low sensitivity of ELISA for diagnosing neurocysticercosis, with a 68.3% rate of false negative.…”