Avaliação dos testes rápidos Optimal-IT® e ICT P.f./P.v.® para o diagnóstico da malária, na Atenção Básica de Saúde, no município de Manaus, Amazonas

Arcanjo, Ana Ruth Lima; Lacerda, Marcus Vinícius Guimarães; Alecrim, Wilson Duarte; Alecrim, Maria das Graças Costa

doi:10.1590/s0037-86822007000100020

Cited by 7 publications

(6 citation statements)

References 9 publications

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“… 11 Operational issues became an obstacle in the use of microscopy in the routine screening of potential donors in blood banks serving large populations and with a high throughput of samples. 10 , 11 In 2010, Ali et al in a study carried out in Pakistan, tested 1558 blood donors using microscopy to diagnose malaria, and found nine (0.577%) positive tests. 13 However, this is not a viable approach for medium and large donation centers.…”

Section: Discussionmentioning

confidence: 99%

“…The immunochromatic antigen-based rapid malaria tests have been developed because of the need of fast and accurate results. 10 Some of these methods utilize monoclonal and polyclonal antibodies directed against the P. falciparum histidine-rich protein 2 (PfHRP-2) and against the Plasmodium lactate dehydrogenase (PLDH) of four Plasmodium species. 11 These are rapid and efficient tests but lack the ability to diagnose mixed infections and present limited sensitivity when parasite levels are low.…”

Section: Discussionmentioning

confidence: 99%

“… 11 These are rapid and efficient tests but lack the ability to diagnose mixed infections and present limited sensitivity when parasite levels are low. 10 …”

Section: Discussionmentioning

confidence: 99%

“…However, this method is limited by its sensitivity and the need of well trained technicians and specialized materials. 10 Even though the thick smear is the most utilized method to diagnose malaria, it cannot be performed as a high throughput test due to the impact on the flow of patient screening in hematology and hemotherapy centers that need to process many patients per day; it is thus unviable in endemic areas. 10 …”

Section: Introductionmentioning

confidence: 99%

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Transfusion-transmitted malaria in endemic zone: epidemiological profile of blood donors at the Fundação HEMOAM and use of rapid diagnostic tests for malaria screening in Manaus

Torres

Moresco

Sales

et al. 2014

Revista Brasileira de Hematologia e Hemoterapia

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ObjectiveWith 99% of the cases in Brazil, malaria is endemic in the Amazon region. Transfusion-transmitted malaria, an important risk in endemic areas, has been reported. The aim of this study was to describe the epidemiological profile of blood donor candidates at the Fundação de Hematologia e Hemoterapia do Amazonas and evaluate the efficacy of rapid diagnostic tests used for malaria screening of blood donors within endemic regions.MethodsBetween May 2008 and May 2009, 407 blood donor candidates were selected and grouped based on the Malaria Annual Parasite Index of the geographic area in which they originated: Group 1 (eligible donors – n = 265) originated from areas of low to medium risk of exposure to malaria and Group 2 (ineligible donors – n = 142) originated from high-risk areas. All samples were concurrently screened using two immunochromatic antigen-based rapid tests and by the thick smear test.ResultsAll samples were negative by all three methods. The demographic profile indicated that the majority of participants were male, ages ranged from 18 to 39 years and less than half the candidates had only elementary schooling. Two issues need to be addressed: one is the ineligibility of donors and its impact on blood donor centers as, in this study, 22.7% of the donors were considered ineligible. The other is the limited sensitivity of the parasitological tests used, allowing a risk of false-negative results.ConclusionNew methods are needed to ensure transfusion safety without rejecting potential donors, which would ensure safe transfusion without harming the blood supply.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“… 11 These are rapid and efficient tests but lack the ability to diagnose mixed infections and present limited sensitivity when parasite levels are low. 10 …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transfusion-transmitted malaria in endemic zone: epidemiological profile of blood donors at the Fundação HEMOAM and use of rapid diagnostic tests for malaria screening in Manaus

Torres

Moresco

Sales

et al. 2014

Revista Brasileira de Hematologia e Hemoterapia

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show abstract

“…However, this method is limited by its sensitivity and its absolute dependence on the operator. Again, blood smears cannot be performed as a high throughput test due to the huge numbers of donor blood units that are processed by blood banks daily [14]. Due to the aforementioned, there is currently no screening test that is fast, practical, affordable and suitably sensitive for blood banks [15].…”

Section: Introductionmentioning

confidence: 99%

Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors

Aninagyei

Smith-Graham

Boye

et al. 2019

Malar J

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Background Undesirable consequences of donor Plasmodium falciparum parasitaemia on stored donor blood have been reported. Therefore, it is imperative that all prospective blood donors are screened for P. falciparum infections using sensitive techniques. In this study, the sensitivities of microscopy, rapid diagnostic test (RDT), loop-mediated isothermal amplification (LAMP) assay and selective whole genome amplification (sWGA) technique in detecting P. falciparum infections in blood donors was assessed. Methods Randomly selected blood donors from 5 districts in Greater Accra Region of Ghana were screened for asymptomatic P. falciparum infections. Each donor sample was screened with SD Bioline RDT kit for P. falciparum histidine rich protein 2 and Plasmodium lactate dehydrogenase antigens, sWGA and 18s-rRNA LAMP. Crude DNA LAMP (crDNA-LAMP) was compared to purified DNA LAMP (pDNA-LAMP). Results A total of 771 blood donors were screened. The respective overall prevalence of P. falciparum in Ghana by microscopy, RDT, crDNA-LAMP, pDNA-LAMP and sWGA was 7.4%, 11.8%, 16.9%, 17.5% and 18.0%. Using sWGA as the reference test, the sensitivities of microscopy, RDT, crDNA-LAMP and pDNA-LAMP were 41.0% (95% CI 32.7–49.7), 65.5% (95% CI 56.9–73.3), 82.6% (95% CI 75.8–88.3) and 95.7% (95% CI 90.1–98.4), respectively. There was near perfect agreement between LAMP and sWGA (sWGA vs. crDNA-LAMP, κ = 0.87; sWGA vs. pDNA-LAMP, κ = 0.96), while crDNA-LAMP and pDNA-LAMP agreed perfectly (κ = 0.91). Goodness of fit test indicated non-significant difference between the performance of LAMP and sWGA (crDNA-LAMP vs. sWGA: x 2 = 0.71, p = 0.399 and pDNA-LAMP vs. sWGA: x 2 = 0.14, p = 0.707). Finally, compared to sWGA, the performance of LAMP did not differ in detecting sub-microscopic parasitaemia (sWGA vs. crDNA-LAMP: x 2 = 1.12, p = 0.290 and sWGA vs. pDNA-LAMP: x 2 = 0.22, p = 0.638). Conclusions LAMP assay agreed near perfectly with sWGA with non-significant differences in their ability to detect asymptomatic P. falciparum parasitaemia in blood donors. Therefore, it is recommended that LAMP based assays are employed to detect P. falciparum infections in blood donors due to its high sensitivity, simplicity, cost-effectiveness and user-friendliness.

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Rapid diagnostic tests for diagnosing uncomplicatedP. falciparummalaria in endemic countries

Abba

Deeks

Olliaro

et al. 2011

Cochrane Database of Systematic Reviews

179

172

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BackgroundRapid diagnostic tests (RDTs) for Plasmodium falciparum malaria use antibodies to detect either HRP-2 antigen or pLDH antigen, and can improve access to diagnostics in developing countries. ObjectivesTo assess the diagnostic accuracy of RDTs for detecting P. falciparum parasitaemia in persons living in endemic areas who present to ambulatory healthcare facilities with symptoms suggestive of malaria by type and brand.

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Avaliação dos testes rápidos Optimal-IT® e ICT P.f./P.v.® para o diagnóstico da malária, na Atenção Básica de Saúde, no município de Manaus, Amazonas

Cited by 7 publications

References 9 publications

Transfusion-transmitted malaria in endemic zone: epidemiological profile of blood donors at the Fundação HEMOAM and use of rapid diagnostic tests for malaria screening in Manaus

Transfusion-transmitted malaria in endemic zone: epidemiological profile of blood donors at the Fundação HEMOAM and use of rapid diagnostic tests for malaria screening in Manaus

Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors

Rapid diagnostic tests for diagnosing uncomplicatedP. falciparummalaria in endemic countries

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