Detection of Mycobacterium leprae infection in wild nine-banded armadillos (Dasypus novemcinctus) using the rapid ML Flow test

Deps, Patrícia Duarte; Antunes, João Marcelo Azevedo de Paula; Tomimori‐Yamashita, Jane

doi:10.1590/s0037-86822007000100019

Cited by 24 publications

(28 citation statements)

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“…31 In another study, Deps et al (2007) found 11 out of 37 (29.7%) positive serum samples using the ML Flow test. 32 Search for M. leprae in wild armadillos D. novemcinctus of different geographical areas resulted negative in Florida, Colombia, Paraguay, [33][34][35] and in the Southeast area of the United States, corresponding to Alabama, Ar- kansas, Florida, Georgia and Mississipi, besides other 800 armadillos examined in Florida. 33,36 In the biodiversity of Latin America fauna, of the actual 20 known armadillo's species, 17 are only found in Brazil and have been little studied.…”

Section: Discussionmentioning

confidence: 95%

“…12,20,21 In that case, however, armadillos would remain for some time with high IgM antibody titles against PGL-1 antigen. The study of Deps et al(2007), using ML-flow, may reflect this situation. 32 The objective of the present study was to identify indigenous leprosy in wild animals, looking for a better understanding of transmission.…”

Section: Discussionmentioning

confidence: 99%

“…The study of Deps et al(2007), using ML-flow, may reflect this situation. 32 The objective of the present study was to identify indigenous leprosy in wild animals, looking for a better understanding of transmission. Some questions are yet to be answered: are armadillos and other animals of South America indeed naturally infected by the M. leprae?…”

Section: Discussionmentioning

confidence: 99%

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Search for Mycobacterium leprae in wild mammals

Pedrini¹,

Rosa²,

Medri³

et al. 2010

Braz J Infect Dis

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Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Search for Mycobacterium leprae in wild mammals

Pedrini¹,

Rosa²,

Medri³

et al. 2010

Braz J Infect Dis

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show abstract

“…Infected nine-banded armadillos have been identified in the states of Texas and Louisiana and in Central and South America (Smith et al 1983, Amezcua et al 1984, Stallknecht et al 1987, Zumarraga et al 2001. Additionally, biomarkers of armadillo infection have been detected in Colombia and Brazil (Deps et al 2007, Cardona-Castro et al 2009). Several studies have shown an association between armadillo exposure through hunting, cleaning and eating the meat and the development of leprosy (Clark et al 2008, Deps et al 2008, Truman 2008.…”

mentioning

confidence: 99%

Mycobacterium leprae in six-banded (Euphractus sexcinctus) and nine-banded armadillos (Dasypus novemcinctus) in Northeast Brazil

Frota

Lima

Rocha

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…Moreover, recent work has shown that M. leprae can be found infecting both humans and wild nine-band arma- dillos in this area (Truman et al 2011) and leprosy transmission by wild fauna, even though the agent was first introduced into the Americas by humans, makes leprosy eradication impossible because this disease may persist as a zoonosis. Wild armadillos naturally infected by leprosy have also been found in different Brazilian regions (Deps et al 2007). A case-control study in Texas found a significant association between leprosy and contact Fig.…”

Section: Discussionmentioning

confidence: 98%