Perfil de isotipos de imunoglobulinas e subclasses de IgG na leishmaniose tegumentar americana

Souza, de; Ag, Da Silva; Afonso-Cardoso,; Sj, Favoreto; Ms, Ferreira

doi:10.1590/s0037-86822005000200002

Cited by 14 publications

(4 citation statements)

References 23 publications

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“…In the literature, the predominance of anti-Leishmania antibodies in visceral leishmaniasis can be explained by the evidence of Th2 response in this disease, with polyclonal activation of B cells and production of specific anti-Leishmania and other antibodies with different specificites. 32,34 Among patients with visceral leishmaniasis, the ANA was undetermined in 8.9% of the cases and positive in 4.4% of the cases (titers above 1/160). Other studies comparing the presence of ANA in patients with visceral or cutaneous leishmaniasis were not found in the literature.…”

Section: Discussionmentioning

confidence: 99%

Frequência de autoanticorpos e dosagem de complemento sérico em pacientes com diagnóstico de leishmaniose cutânea ou visceral

Horimoto¹,

Costa²

2009

Rev. Bras. Reumatol.

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Introduction: Leishmaniasis is a chronic infectious disease whose spectrum can vary from isolate cutaneous involvement with oligosymptomatic manifestations to systemic involvement with clinically important manifestations. The development of the infection of each type of leishmaniasis (visceral or cutaneous) depends on a complex and intriguing interaction between virulence factors of the pathogen and the immune response of the host. Analysis of sera of with Leishmania infection demonstrates the presence of autoantibodies against cellular and humoral components, besides circulating immune complexes and anti-IgG antibodies (rheumatoid factor). Patients with visceral leishmaniasis can present symptoms that mimic Systemic Lupus Erythematosus (SLE), hindering early diagnosis and treatment. Objectives: To identify the profile of autoantibodies and complement levels of patients with visceral or cutaneous leishmaniasis and to correlate their clinical presentation to those of patients with SLE. Methods: The presence of autoantibodies and complement levels of 90 patients, 45 with visceral leishmaniasis and 45 with cutaneous leishmaniasis, was determined. Results: The presence of statistically significant autoantibodies in patients with visceral leishmaniasis included: antinuclear antibody (ANA), positive (4.4%) or in low titers (8.9%), and IgG anticardiolipin antibody, positive (17.8%) or undetermined (8.9%). A reduction in C3 levels was also seen in 17.8% of the patients and anti-Leishmania antibodies > 1/80 in all patients with visceral leishmaniasis. Conclusions: Visceral leishmaniasis can have a positive correlation with the presence of autoantibodies, possibly by triggering a predominantly humoral, systemic, type Th2 response, representing an obligatory differential diagnosis with SLE, especially in endemic areas.

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Section: Discussionmentioning

confidence: 99%

Frequência de autoanticorpos e dosagem de complemento sérico em pacientes com diagnóstico de leishmaniose cutânea ou visceral

Horimoto¹,

Costa²

2009

Rev. Bras. Reumatol.

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“…é a capacidade do parasito em desviar e subverter as respostas imunes do hospedeiro (GURUNG; KANNEGANTI, 2015). A resposta imune inata é essencial para a eliminação de patógenos invasores, embora o mecanismo protetor da LTA seja dependente das células T (TRUJILLO et al, 2000;SOUZA et al, 2005).…”

Section: Introductionunclassified

“…Nas leishmanioses cutânea e mucocutânea a resposta do tipo Th1 tem sido associada com a presença de isotipos IgG1, IgG2 e IgG3. A resposta do tipo Th2 está vinculada com a presença de IgG4 para a leishmaniose cutânea difusa (SOUZA et al, 2005;).…”

Section: Introductionunclassified

AVALIAÇÃO DO ISOTIPO IgG1 e IgG2 POR CITOMETRIA DE FLUXO EM PACIENTES COM LTA

Pereira¹,

Oliveira²,

Oliveira³

et al. 2017

Anais Do 5º Encontro Brasileiro Para Inovação Terapêutica

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“…In dogs, four IgG subclasses have been defined 33,34,35 , which can be applied to the diagnosis of infectious diseases, including leishmaniasis 24,52 . The study of IgG subclasses in canine AVL might have a practical application both to the diagnosis and to the development of control strategies 50 .…”

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confidence: 99%

Use of elisa employing homologous and heterologous antigens for the detection of IgG and subclasses (IgG1 and IgG2) in the diagnosis of Canine visceral leishmaniasis

Ribeiro

Schubach

Mouta-Confort

et al. 2011

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIndirect immunofluorescence is the method recommended for the diagnosis of visceral leishmanisis in dogs, however, the accuracy of this technique is low and its use on a large scale is limited. Since ELISA does not present these limitations, this technique might be an option for the detection of IgG or specific IgG1 and IgG2 subclasses. Canine ehrlichiosis is an important differential diagnosis of American Visceral Leishmaniasis (AVL). The present study compared ELISA using Leishmania chagasi and Leishmania braziliensis antigen for the detection of anti-Leishmania IgG and subclasses in serum samples from 37 dogs naturally infected with L. chagasi (AVL) and in samples from four dogs co-infected with L. braziliensis and L. chagasi (CI). The occurrence of cross-reactivity was investigated in control serum samples of 17 healthy dogs (HC) and 35 infected with Ehrlichia canis (EC). The mean optical density obtained for the detection of IgG was significantly higher when L. chagasi antigen was used, and was also higher in subgroup VLs (symptomatic) compared to subgroup Vla (asymptomatic). The correlation between IgG and IgG1 was low. The present results suggest that IgG ELISA using homologous antigen yields the best results, permitting the diagnosis of asymptomatic L. chagasi infection and the discrimination between cases of AVL and ehrlichiosis in dogs.

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Perfil de isotipos de imunoglobulinas e subclasses de IgG na leishmaniose tegumentar americana

Cited by 14 publications

References 23 publications

Frequência de autoanticorpos e dosagem de complemento sérico em pacientes com diagnóstico de leishmaniose cutânea ou visceral

Frequência de autoanticorpos e dosagem de complemento sérico em pacientes com diagnóstico de leishmaniose cutânea ou visceral

AVALIAÇÃO DO ISOTIPO IgG1 e IgG2 POR CITOMETRIA DE FLUXO EM PACIENTES COM LTA

Use of elisa employing homologous and heterologous antigens for the detection of IgG and subclasses (IgG1 and IgG2) in the diagnosis of Canine visceral leishmaniasis

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