Epidemiological factors related to the transmission risk of Trypanosoma cruzi in a Quilombola community, State of Mato Grosso do Sul, BrazilThe parasitological tests showed flagellates in an opossum and two cattle. The molecular tests showed DNA from T. cruzi in an opossum and a pig. Triatoma sordida was the only triatomine species found in the community, and it colonized households (four specimens) and the surrounding areas (124 specimens). Twentythree specimens tested positive for flagellates, which were subsequently identified as T. cruzi by PCR. Conclusions: Data analysis demonstrated that T. cruzi has a peridomestic life cycle that involves both domestic and wild mammals.