Avaliação terapêutica do artesunato na infecção experimental pelo Schistosoma mansoni

Araújo, Neusa; Kohn, Anna; Katz, Naftale

doi:10.1590/s0037-86821999000100002

Cited by 17 publications

(11 citation statements)

References 5 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the animals treated with 300 mg/kg or 600 mg/ kg artesunate, 14 or 21 days post-infection, a small percentage of oogram changes could be observed, although not statistically significant, is of relevance. According to Araújo et al (1999), treatment with artesunate, at the postural phase, is capable of interrupting egg-laying only temporarily.…”

Section: Discussionmentioning

confidence: 99%

Schistogram changes after administration of antischistosomal drugs in mice at the early phase of Schistosoma mansoni infection

Vimieiro¹,

Araújo²,

Katz³

et al. 2013

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

Mice infected with Schistosoma mansoni were treated with oxamniquine, praziquantel, artesunate at the pre-patent phase, aiming at observing schistogram alterations. Half of the animals were perfused five days post-treatment for counting and classification of immature worms, based on pre-established morphological criteria (schistogram); the remaining animals were evaluated 42 or 100 days after infection and perfusion of the portal-system was performed for collection and counting of adult worms and oogram. It was observed that oxamniquine and artesunate treatment administered at the pre-postural phase causes significant reduction in the number of immature and adult worms. However, there was little reduction with praziquantel when used at the dose of 400 mg/kg for treatments administered 14, 15, 21 or 23 days post-infection. Artesunate was responsible for significant alterations in development of young worms, as well as for a higher number of worms presenting intestinal damages. Immature adult worms were detected in mice treated with artesunate or oxamniquine at the pre-patent phase of infection and recovered by perfusion 100 days after infection. Schistogram proved to be a very useful tool for experimental evaluation of the activity of antischistosomal drugs and a good model to identify the most sensitive stages to drugs.

show abstract

Section: Discussionmentioning

confidence: 99%

Schistogram changes after administration of antischistosomal drugs in mice at the early phase of Schistosoma mansoni infection

Vimieiro¹,

Araújo²,

Katz³

et al. 2013

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to their blood habit, adult schistosomes may coexist with a large number of harmful factors (Skelly et (Araújo et al, 1999(Araújo et al, , 2002. However, the use of these drugs in a multiple-dose regimen has not been reported so far in mice fed high-fat chow.…”

Section: Discussionmentioning

confidence: 99%

“…Drug-induced morphological damages were characterized by alterations of reproductive organs (cellular alterations in vitelline glands and reduced ovary). In addition, oral administration of 300 mg/kg for five consecutive days induced greater hepatic shift and worm death compared to the single dose (Araújo et al, 1999).…”

Section: Introductionmentioning

confidence: 86%

Effects of multiple doses of simvastatin and artesunate monotherapy on SCHISTOSOMA MANSONI adult worms during infection in hyperlidemic mice

et al. 2021

View full text Add to dashboard Cite

A single dose of simvastatin and of artesunate monotherapy cause damage to the reproductive system of schistosomes as well as severe tegumental damage in male worms recovered from mice fed high-fat chow. This study aims to investigate whether treatment with multipledose regimes may offer more antischistosomal activity advantages than single daily dosing in mice fed high-fat chow. For this purpose, nine weeks post-infection, Swiss Webster mice were gavaged with simvastatin (200 mg/kg) or artesunate (300 mg/kg) for five consecutive days and euthanized two weeks post-treatment. Adult worms were analyzed using brightfieldmicroscopy, confocal microscopy and scanning electron microscopy, presenting damages caused by simvastatin and artesunate to the reproductive system of males and females as well as tegument alterations, including peeling, sloughing areas, loss of tubercles, tegumental bubbles and tegument rupture exposing subtegumental tissue. The overall findings in this study revealed the potential antischistosomal activity of simvastatin and artesunate against Schistosoma mansoni adult worms, in addition to showing that multiple doses of either monotherapy caused severe damage to the tegument.KEY WORDS: Schistosoma mansoni; hyperlipidemia; simvastatin; artesunate; microscopy.

show abstract

“…Degeneration of the ovary of schistosomes is well known to occur under various conditions, such as in vitro cultivation (Shaw, 1977 ;Irie, Basch & Beach, 1983), separation of male and female worms (reviewed by Ribeiro-Paes & Rodrigues, 1997 ;Kunz, 2001), and treatment with various drugs (Davies & Jackson, 1970 ;Irie et al 1989 ;Araujo, Kohn & Katz, 1999). Atrophy of the ovary and of the vitelline glands is normally related, and coincides with a decrease in worm length.…”

Section: Discussionmentioning

confidence: 99%

Morphological changes in the reproductive organs of male and female Schistosoma mansoni worms caused by streptozotocin, a drug used to induce diabetes mellitus

et al. 2003

View full text Add to dashboard Cite

Host metabolic changes have been observed to affect Schistosoma mansoni egg production, worm survival and morphology. We examined worms recovered from streptozotocin-induced diabetic mice by morphometric and morphological analysis through brightfield and confocal laser scanning microscopy. Tegument thickness was slightly smaller and changes in the reproductive organs were observed in 23-30% and consecutively 88-100% of the worms. The testicular lobes had a large diminution of cells in one or more of the lobes, which was associated with a lack of spermatozoids in the seminal vesicle. Ovaries were atrophied, manifested by a complete or large reduction in oocytes but other parts of the reproductive system like the vitelline glands were mainly unaffected. Streptozotocin (STZ) instead of hyperglycaemia caused the degeneration since worms from mice injected with a non-diabetogenic dose, or with nicotinamide to prevent diabetes showed the same alterations. The drug did not affect worm survival or pairing. We conclude that STZ, an alkylating agent that provokes chromosome and DNA damage, changes the morphology of ovaries and testicular lobes in S. mansoni worms in vivo. This is the first report of STZ action in helminths and we suggest that STZ affects oogenesis and spermatogenesis and might cause sterilization of schistosomes.

show abstract

Avaliação terapêutica do artesunato na infecção experimental pelo Schistosoma mansoni

Abstract: Resumo Camundongos infectados experimentalmente com

Cited by 17 publications

References 5 publications

Schistogram changes after administration of antischistosomal drugs in mice at the early phase of Schistosoma mansoni infection

Schistogram changes after administration of antischistosomal drugs in mice at the early phase of Schistosoma mansoni infection

Effects of multiple doses of simvastatin and artesunate monotherapy on SCHISTOSOMA MANSONI adult worms during infection in hyperlidemic mice

Morphological changes in the reproductive organs of male and female Schistosoma mansoni worms caused by streptozotocin, a drug used to induce diabetes mellitus

Contact Info

Product

Resources

About