Morphological changes in the reproductive organs of male and female <i>Schistosoma mansoni</i> worms caused by streptozotocin, a drug used to induce diabetes mellitus

Hulstijn, Maarten; Barros, Luciano Antunes; Neves, Renata Heisler; Moura, Egberto Gaspar de; Machado-Silva, José Roberto

doi:10.1017/s0031182002002512

Cited by 20 publications

(14 citation statements)

References 26 publications

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“…The lobes of these supernumerary testes contain variable amounts of fully developed oocytes instead of a testicular mass (Hulstijn et al, 2003). The lobes of these supernumerary testes contain variable amounts of fully developed oocytes instead of a testicular mass (Hulstijn et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Light and confocal microscopic observations of adultSchistosoma mansonifrom mice fed on a high-fat diet

Ac²,

et al. 2007

J. Helminthol.

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The morphological aspects of Schistosoma mansoni adult worms recovered from albino mice fed on a cholesterol-rich diet compared to mice fed on a standard chow were investigated. After feeding on their respective diets for over a period of 5 months, mice were subcutaneously infected with c. 50 S. mansoni cercariae/mouse. Blood samples were obtained 1 day prior to experimental infections and 63 days later, when mice were euthanized by jugular section (hypovolaemic shock). Total cholesterol (TC) levels were determined. Recovered worms were stained with hydrochloric carmine, and preserved as whole-mounts for examination by bright-field and laser confocal microscopy. The infected mice fed on high-fat chow showed higher levels of serum lipoproteins than the infected mice fed on standard chow, except for very-low-density lipoprotein cholesterol (VLDL-c) and triglycerides (TG). In this experiment, worms from mice fed on a high-fat chow showed a greater percentage of morphological differentiation regarding supernumerary testes, seminal vesicle, and seminal receptacle. In mice of this group, the rate of oocyte laying in the ovary was much higher than in control females. The present results suggest that cholesterol could be actively involved in the modulation of cell signalling and reproduction, because the lobes contained fully developed oocytes in variable amounts, different from control males. The data presented here are the first to report the role of a cholesterol-rich diet affecting the development of S. mansoni worms.

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Section: Discussionmentioning

confidence: 99%

Light and confocal microscopic observations of adultSchistosoma mansonifrom mice fed on a high-fat diet

Ac²,

et al. 2007

J. Helminthol.

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“…Different endohelminths of fish were suggested as sentinel organisms to detect metal pollution in aquatic habitats [1]. Most of the available papers emphasize adult intestinal parasites such as acanthocephalans (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the available papers emphasize adult intestinal parasites such as acanthocephalans (e.g. Pomphorhynchus laevis, Acanthocephalus lucii ), which have a remarkable capacity to accumulate heavy metals [1,2] at concentrations many hundred to a thousand times higher than their host tissues and the aqueous environment [3,4]. With the use of these parasites even low levels of environmental pollution can be detected [5], which is often impossible using conventional analytical methods and/or free living sentinels such as mussels [6].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the metal accumulation capacity between the acanthocephalan Pomphorhynchus laevis and larval nematodes of the genus Eustrongylides sp. infecting barbel (Barbus barbus)

2013

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BackgroundMetal uptake and accumulation in fish parasites largely depends on the parasite group with acanthocephalans showing the highest accumulation rates. Additionally, developmental stage (larvae or adult) as well as parasite location in the host are suggested to be decisive factors for metal bioconcentration in parasites. By using barbel (Barbus barbus) simultaneously infected with nematode larvae in the body cavity and adult acanthocephalans in the intestine, the relative importance of all of these factors was compared in the same host.MethodsEleven elements Arsenic (As), Cadmium (Cd), Cobalt (Co), Copper (Cu), Iron (Fe), Manganese (Mn), Lead (Pb), Selenium (Se), Tin (Sn), Vanadium (V) and Zinc (Zn) were analyzed in barbel tissues (muscle, intestine, liver) as well as in their acanthocephalan parasites Pomphorhynchus laevis and the larval nematode Eustrongylides sp. (L4) using inductively coupled plasma mass spectrometry (ICP-MS).ResultsNine elements were detected in significantly higher levels in the parasites compared to host tissues. The element composition among parasites was found to be strongly dependent on parasite taxa/developmental stage and localization within the host. Intestinal acanthocephalans accumulated mainly toxic elements (As, Cd, Pb), whereas the intraperitoneal nematodes bioconcentrated essential elements (Co, Cu, Fe, Se, Zn).ConclusionOur results suggest that in addition to acanthocephalans, nematodes such as Eustrongylides sp. can also be applied as bioindicators for metal pollution. Using both parasite taxa simultaneously levels of a wide variety of elements (essential and non essential) can easily be obtained. Therefore this host-parasite system can be suggested as an appropriate tool for future metal monitoring studies, if double infected fish hosts are available.

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“…Schistosomes are blood-dwelling flukes that are highly dependent on the host metabolism (Saule et al 2005). As a result, physiological changes associated with schistosomiasis infection have been considered important factors influencing this host-parasite relationship (Hulstijn et al 2003). Early studies showed dramatic alterations in adult worm development (Neves et al 2001, as well as diminished schistosomal periovular granuloma and milder chronic lesions in undernourished mice compared to mice fed a normal diet .…”

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confidence: 99%

High fat diet has a prominent effect upon the course of chronic schistosomiasis mansoni in mice

Alencar

Neves

Águila³

et al. 2009

Mem. Inst. Oswaldo Cruz

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This study investigated whether a long-term high-fat diet has an effect on the outcome of chronic murine schistosomiasis mansoni compared to a standard diet. Swiss Webster female mice (3 weeks old) were fed each diet for up to six months and were then infected with 50 Schistosoma mansoni cercariae. Their nutritional status was assessed by monitoring total serum cholesterol and body mass. Infected mice were examined 6-17 weeks post infection to estimate the number of eggs in faeces. Mice were euthanised the next day. Total serum cholesterol was lower in infected mice in comparison to uninfected controls (p = 0.0055). In contrast, body mass (p = 0.003), liver volume (p = 0.0405), spleen volume (p = 0.0124), lung volume (p = 0.0033) and faecal (p = 0.0064) and tissue egg density (p = 0.0002) were significantly higher for infected mice fed a high-fat diet. From these findings, it is suggested that a high-fat diet has a prominent effect on the course of chronic schistosomiasis mansoni in mice.

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Morphological changes in the reproductive organs of male and female Schistosoma mansoni worms caused by streptozotocin, a drug used to induce diabetes mellitus

Cited by 20 publications

References 26 publications

Light and confocal microscopic observations of adultSchistosoma mansonifrom mice fed on a high-fat diet

Light and confocal microscopic observations of adultSchistosoma mansonifrom mice fed on a high-fat diet

Comparison of the metal accumulation capacity between the acanthocephalan Pomphorhynchus laevis and larval nematodes of the genus Eustrongylides sp. infecting barbel (Barbus barbus)

High fat diet has a prominent effect upon the course of chronic schistosomiasis mansoni in mice

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