Controle do calazar canino: comparação dos resultados de um programa de eliminação rápida de cães sororreagentes por ensaio imuno-enzimático com outro de eliminação tardia de cães sororreagentes por teste de imunofluorescência indireta de eluato de papel filtro

Abstract

The kala-azar control program, adopted by the Fundação Nacional de Saúde-FNS (National Health Foundation) has not been able to reduce to an acceptable level the incidence of human cases. The diagnostic method utilized is a blood eluate immunofluorescence. A dogs diagnosed as infected is eliminated a mean of eighty days after the blood collection. The low sensitivity of the test used and the continuing residence of the infected dog in the region due to the elimination delay may be critical in the lack of success of this program. In this study, the FNS standard canine control method is compared to a strategy based on ELISA identification of infected dog and elimination within 7 days. In both study areas the canine seroprevalence was noted ten months before and ten months after the intervention. In the routine FNS area a 9% decrease in seroprevalence was noted, compared to statistically significant greater 27%, reduction (p = 0.0015) in the ELISA intervention area.

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

“…The first is related to the number of infected dogs that remain after culling of seropositive animals and that are not detected by other tests, and the second refers to the unnecessary sacrifice of dogs due to the large number of false-positive IFAT reactions in serum. BRAGA et al 4 demonstrated that up to 2.85 times more seropositive dogs are detected by ELISA using serum samples than by IFAT testing of eluate samples.…”

American visceral leishmaniasis: disease control strategies in dracen microregion in alta paulista, SP, Brazil

“…Afterwards, the sample will be subject only to IFAT method (14). According to the suppliers of anti-Leishmania antibody tests, there is an inconsistency in use procedures to obtain a final result, regarding sensitivity and specificity, which determines whether or not the dog will be killed, a problem also reported by Braga et al (32). Since IFAT presents greater sensitivity, it would be more useful in the "sorting", while ELISA -with greater specificity -would guarantee the accuracy of the infection suspicion.…”

Visceral leishmaniasis in Brazil: revisiting paradigms of epidemiology and control

“…These control strategies have remained unchanged since the 1950's 20 , and have not been able to reduce the incidence of human cases to an acceptable level 8,14 . Indeed, the prevalence of VL has been increased and the disease has become a serious public health problem in several Brazilian states.…”