Análise quantitativa de mastócitos e de granulócitos eosinófilos no miocárdio de ratos wistar cronicamente infectados pelo Trypanosoma cruzi. Contribuição ao conhecimento da fibrose miocárdia

Pinheiro, Maria da Conceição Nascimento; Beraldo, Paulo Sérgio Siebra; Júnior, Luiz Fernando Junqueira; Lopes, Edison Reis; Chapadeiro, E

doi:10.1590/s0037-86821992000100007

Cited by 6 publications

(1 citation statement)

References 6 publications

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“…Our data are in agreement with quantitative studies of mast cells in the human myocardium 7 and in the myocardium of rats with chronic Chagas' disease via experimental infection 12 . The data also agree with the description of increased mast cell counts in the circular musculature of the small and large intestines in mice with chronic Trypanosoma cruzi infection 14 .…”

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confidence: 89%

Morphometric study of the fibrosis and mast cell count in the circular colon musculature of chronic Chagas patients with and without megacolon

Pinheiro

Rua

Etchebehere

et al. 2003

Rev. Soc. Bras. Med. Trop.

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A morphometric study of the circular colon musculature was performed, in which the mast cell count was determined and the connective fibrous tissue in this layer was measured. The objective was to gain better understanding of Chagas megacolon morphology and contribute towards the knowledge of fibrosis pathogenesis in Chagas megas. An evaluation was made of 15 distal sigmoid rings from Chagas patients with megacolon (MCC), 15 without megacolon (CSMC) and 15 non-Chagas patients (NC). The rings were fixed in formol, embedded in paraffin, and 7mm thick sections were cut and stained using Azan-Heidenhain and Giemsa. The mast cell count and fibrosis were greater in the MCC group than in the CSMC and NC groups (p < 0.05; Kruskal-Wallis test) and there was no significant difference between the latter two. The fibrosis and increased mast cell count in the colon musculature of the MCC group possibly indicates that there is a relationship between mastocytosis and fibrosis, as has already been demonstrated in other pathologies.

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Section: Discussionsupporting

confidence: 89%

Morphometric study of the fibrosis and mast cell count in the circular colon musculature of chronic Chagas patients with and without megacolon

Pinheiro

Rua

Etchebehere

et al. 2003

Rev. Soc. Bras. Med. Trop.

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Mast cell-nerve interaction in the colon of Trypanosoma cruzi-infected individuals with chagasic megacolon

et al. 2018

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Chagas disease is an infection caused by the parasite Trypanosoma cruzi that affects millions of people worldwide and is endemic in Latin America. Megacolon is the most frequent complication of the digestive chronic form and happens due to lesions of the enteric nervous system. The neuronal lesions seem to initiate in the acute phase and persist during the chronic phase, albeit the mechanisms involved in this process are still debated. Among the cells of the immune system possibly involved in this pathological process is the mast cell (MC) due to its well-known role in the bi-directional communication between the immune and nervous systems. Using ultrastructural analysis, we found an increased number of degranulated MCs in close proximity to nerve fibers in infected patients when compared with uninfected controls. We also immunostained MCs for the two pro-inflammatory molecules tryptase and chymase, the first being also important in neuronal death. The number of MCs immunostained for tryptase or chymase was increased in patients with megacolon, whereas increased tryptase staining was additionally observed in patients without megacolon. Moreover, we detected the expression of the tryptase receptor PAR2 in neurons of the enteric nervous system, which correlated to the tryptase staining results. Altogether, the data presented herein point to the participation of MCs on the denervation process that occurs in the development of T. cruzi-induced megacolon.

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Mast Cell Function and Death in Trypanosoma cruzi Infection

Meuser-Batista

Corrêa

Carvalho

et al. 2011

The American Journal of Pathology

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Although the roles of mast cells (MCs) are essential in many inflammatory and fibrotic diseases, their role in Trypanosoma cruzi-induced cardiomyopathy is unexplored. In this study, we treated infected CBA mice with cromolyn, an MC stabilizer, and observed much greater parasitemia and interferon-γ levels, higher mortality, myocarditis, and cardiac damage. Although these data show that MCs are important in controlling acute infection, we observed MC apoptosis in the cardiac tissue and peritoneal cavity of untreated mice. In the heart, pericardial mucosal MC die, perhaps because of reduced amounts of local stem cell factor. Using RT-PCR in purified cardiac MCs, we observed that infection induced transcription of P2X(7) receptor and Fas, two molecules reportedly involved in cell death and inflammatory regulation. In gld/gld mice (FasL(-/-)), apoptosis of cardiac, but not peritoneal, MCs was decreased. Conversely, infection of P2X(7)(-/-) mice led to reduced peritoneal, but not cardiac, MC death. These data illustrate the immunomodulatory role played by MCs in T. cruzi infection and the complexity of molecular interactions that control inflammatory pathways in different tissues and compartments.

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Análise quantitativa de mastócitos e de granulócitos eosinófilos no miocárdio de ratos wistar cronicamente infectados pelo Trypanosoma cruzi. Contribuição ao conhecimento da fibrose miocárdia

Cited by 6 publications

References 6 publications

Morphometric study of the fibrosis and mast cell count in the circular colon musculature of chronic Chagas patients with and without megacolon

Morphometric study of the fibrosis and mast cell count in the circular colon musculature of chronic Chagas patients with and without megacolon

Mast cell-nerve interaction in the colon of Trypanosoma cruzi-infected individuals with chagasic megacolon

Mast Cell Function and Death in Trypanosoma cruzi Infection

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