Sobre a sensibilidade da cultura de leucócitos circulantes na detecção de Leishmania no sangue periférico de pacientes com leishmaniose tegumentar

Sílveira, Fernando Tobias; Lainson, Ralph; Shaw, Jeffrey Jon; Ishikawa, Edna Aoba Yassui; Souza, Álvaro Nogueira de; Braga, R. R.

doi:10.1590/s0037-86821989000300006

Cited by 13 publications

(4 citation statements)

References 4 publications

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“…Involvement of mucosae and multiple cutaneous sites may also result from the dissemination of the parasite through the blood or lymphatic route as suggested for human infection (Martinez et al 1992;Sousa et al 1995 ;Weigle and Saravia 1996). In the present study there was no evidence of dissemination of Leishmania through blood cultures, suggesting that blood dissemination should be an eventual or intermittent phenomenon (Bowdre et al 1981;Martinez et al 1992;Silveira et al 1989;Sousa et al 1995). Other authors tested whole blood, buffy coat and bone marrow aspirates with the more-sensitive method PCR to detect L. (Viannia) infection in both asymptomatic and symptomatic dogs (Reithinger et al 2003).…”

Section: Discussionsupporting

confidence: 53%

Is Leishmania (Viannia) braziliensis preferentially restricted to the cutaneous lesions of naturally infected dogs?

Madeira

Schubach

et al. 2005

Parasitol Res

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Nineteen dogs naturally infected with Leishmania (Viannia) braziliensis were studied in order to determine the presence of the parasite outside cutaneous lesions. Eleven (57.9%) animals showed single cutaneous or mucosal lesions and eight (42.1%) presented two or three lesions. Twenty-eight active lesions were biopsied. Isolation in culture and characterization by enzyme electrophoresis were possible in 100% of cases and amastigote forms were visualized upon histopathological examination in three samples (n=25, 12%). Isolation of the parasite in culture from peripheral blood and intact skin fragments obtained from the scapular region was negative in all animals, as was the histopathological analysis of skin from this region. Serological reactivity determined by an immunofluorescent antibody test and/or enzyme-linked immunosorbent assay was demonstrated in 15 animals. The results obtained suggest that L. braziliensis preferentially remains at the site of lesion, in contrast to the systemic distribution of parasites observed in dogs infected with L. (Leishmania) chagasi. A better understanding of this aspect may help direct diagnostic and control strategies applicable to areas characterized by the simultaneous occurrence of the cutaneous and visceral forms of leishmaniasis, as is the case for the Municipality of Rio de Janeiro, Brazil.

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Section: Discussionsupporting

confidence: 53%

Is Leishmania (Viannia) braziliensis preferentially restricted to the cutaneous lesions of naturally infected dogs?

Madeira

Schubach

et al. 2005

Parasitol Res

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“…Attempts to isolate other dermotropic species in human peripheral blood have been unsuccessful (43).…”

Section: Parasite Persistencementioning

confidence: 99%

Anthropogenic Dispersal of Leishmania (Viannia) braziliensis in the Americas: A Plausible Hypothesis

Marzochi

Fagundes

et al. 2021

Front. Trop. Dis

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There are several gaps in our knowledge on the origin and spread of Leishmania (Viannia) braziliensis, an etiological agent of cutaneous and mucocutaneous or American tegumentary leishmaniasis, to different biomes, hosts, and vectors, with important epidemiological implications, including the possible existence of an anthroponotic component. Historical, biological, and epidemiological evidence suggests that Leishmania (V.) braziliensis and its variants were preexistent in Amazonia with great genetic variability, where they dispersed with less variability to other regions (clonal expansion). During pre-Columbian times the parasite may have been transported by migrating humans and probably also their dogs, from western Amazonia to the high inter-Andean valleys and from there to other regions of South America. The same thing could have happened later, in the same way, when it spread to non-Amazonian regions of Brazil and other countries of South and Central America, between the late 19th and early 20th centuries, during the so-called Rubber Boom and construction of the Madeira-Mamoré Railway in the Brazilian Amazon, by migrant workers who later returned to their places of origin, transporting the agent. The parasite’s dispersal in genetic correlated clusters, involving unexpectedly distinct ecosystems in Brazil (Amazonian, Cerrado, Caatinga and Atlantic Forest biomes), has continued until the present through human displacement. The infection of certain species of domestic, synanthropic and even wild animals, could be secondary to anthropogenic introduction of L. (V.) braziliensis in new environments. We admit the same phenomena happening in the probable transference of Leishmania infantum (visceral leishmaniasis), and of Yersinia pestis (plague) from the Old world to the New world, generating domestic and wild enzotic cycles from these agents. These assumptions associated with human infections, chronicity and parasite persistence with possibility of recovery of Leishmania in peripheral blood, skin and scars of cured or asymptomatic patients, (that may provide an alternative blood meal), along with the sand flies’ adaptation to the peri-domicile and the high susceptibility of domestic dogs, horses, mules and cats to the parasite, can reinforce the evidence of anthropogenic spread of L. (V.) braziliensis.

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“…As arboviroses formam um grupo heterogêneo de vírus ecologicamente bem definidos, sendo mantidos em natureza entre vetores artrópodes hematófagos e hospedeiros vertebrados susceptíveis, em especial animais silvestres, sendo o homem geralmente o hospedeiro acidental (Silveira et al, 2013). No entanto, alguns arbovírus, como os vírus Dengue (DENV), vírus Chikungunya (CHIKV) e vírus Zika (ZIKV), se adaptaram ao ciclo de transmissão urbana, em especial aqueles transmitidos pelo mosquito Aedes aegypti, onde a transmissão ocorre entre homem-vetor devido ao caráter antropofílico desse mosquito e sua alta adaptação de reprodução em ambientes urbanos (Terra et al, 2017).…”

Section: Introductionunclassified

Caracterização do perfil epidemiológico e clínico dos casos de arboviroses urbanas transmitidas pelo Aedes no Brasil, nos anos de 2017 a 2021

Rios

Ferreira

Azevedo

et al. 2023

RSD

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As infecções por arbovírus aumentaram dramaticamente durante os últimos anos. A sintomatologia semelhante na fase inicial da doença dificulta o diagnóstico diferencial e o manejo clínico. Esse estudo descreve o perfil epidemiológico e clínico dos casos de arboviroses urbanas transmitidas por Aedes aegypti no Brasil no período de 2017 a 2021. Foram analisados 1.145 casos humanos disponíveis no Sistema de Gerenciador de Ambiente Laboratorial (GAL) positivos para os vírus Chikungunya (CHIKV) (741/64,71%), vírus Dengue (DENV) (381/33,27%) e vírus Zika (ZIKV) (23/2%). As informações clínicas e epidemiológicas foram obtidas a partir das fichas de investigação epidemiológicas do Sistema de Informação de Agravos de Notificação (SINAN). A maioria dos infectados eram do sexo feminino (63%), pardos (31%) e faixa etária de 31 a 40 anos de idade. Os sintomas mais relatados foram febre (90%), artralgia intensa (67,3%), cefaleia (53,2%), mialgia (52,8%) e exantema (50%). Durante a fase aguda (D0 a D6) foi observado que os indivíduos infectados com DENV apresentavam sinais/sintomas mais elevados dos que os infectados com o ZIKV e CHIKV. Na fase de convalescência (D7 a >D15), os sinais/sintomas dos infectados com CHIKV elevaram-se e os com DENV e ZIKV declinaram. Quando se considerou a avaliação dos sinais/sintomas em uma proporção aumentada (≥10%) foram observados entre os infectados com o CHIKV os sinais/sintomas apresentaram-se em uma proporção aumentada mais tardiamente (D6 a >D15). A caracterização do perfil clínico das arboviroses pode direcionar o diagnóstico clínico diferencial, bem como, correlacionar os aspectos epidemiológicos com a evolução da doença.

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Sobre a sensibilidade da cultura de leucócitos circulantes na detecção de Leishmania no sangue periférico de pacientes com leishmaniose tegumentar

Cited by 13 publications

References 4 publications

Is Leishmania (Viannia) braziliensis preferentially restricted to the cutaneous lesions of naturally infected dogs?

Is Leishmania (Viannia) braziliensis preferentially restricted to the cutaneous lesions of naturally infected dogs?

Anthropogenic Dispersal of Leishmania (Viannia) braziliensis in the Americas: A Plausible Hypothesis

Caracterização do perfil epidemiológico e clínico dos casos de arboviroses urbanas transmitidas pelo Aedes no Brasil, nos anos de 2017 a 2021

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