FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL

Abstract

Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized patients. The Candida isolates included in this study were obtained from blood cultures, abdominal fluids, and central venous catheters (CVC) of hospitalized patients at the Clinical Hospital of the Federal University of Uberlândia during the period of July 2010 - June 2011. Susceptibility tests were conducted by the broth microdilution method. The RAPD-PCR tests used employed initiator oligonucleotides OPA09, OPB11, and OPE06. Of the 63 Candida isolates, 18 (28.5%) were C. albicans, 20 (31.7%) were C. parapsilosis complex species, 14 (22.2%) C. tropicalis, four (6.4%) C. glabrata, four (6.4%) C. krusei, two (3.3%) C. kefyr, and one (1.6%) C. lusitaniae. In vitro resistance to amphotericin B was observed in 12.7% of isolates. In vitroresistance to azoles was not detected, except for C. krusei. The two primers, OPA09 and OPB11, were able to distinguish different species. Isolates of C. albicans and C. parapsilosis complex species presented six and five clusters, respectively, with the OPA09 marker by RAPD-PCR, showing the genetic variability of the isolates of those species. It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole. High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker.

Antifungal Resistance of Candida Species in Bacolod City, Philippines

“…This is because C. albicans is the most common and most pathogenic [20] among Candida species [21] worldwide [22]. The findings of this study are similar to the study of Menezes [23], Bhattacharjee [9], and Lima [3]. Further noted in other studies, non-albicans Candida were starting to rise in numbers [9,24] and seen as a suggestive emergence of important pathogens.…”

Candidemia by Candida parapsilosis in a neonatal intensive care unit: human and environmental reservoirs, virulence factors, and antifungal susceptibility

“…These results are higher than those observed in previous studies conducted in the same NICU, where the frequency of Candida spp. was below 10% [7,29]. This can be related to the admission of newborns with severe health condition, especially because most newborns of the study were extremely low weight.…”

“…The C. parapsilosis complex has emerged as a major cause of candidemia worldwide among non-albicans species [7,8]. In addition, it is associated with several outbreaks of NICU and high morbidity and mortality rates [6,9,10].…”

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA†

“…Quite a few of the reports deemed relevant in the ELS (43) presented information demonstrating a low or very low prevalence (seldom above 5%) of K. marxianus among fungi (mainly Candida spp.) isolated from patients with various serious underlying diseases, like cancer or HIV, or other immunosuppressed states, and patients with catheters (de Freitas et al., ; Ghahri et al., ; Parmeland et al., ; Abrantes et al., ; Dufresne et al., ; Haddadi et al., ; Taj‐Aldeen et al., ; Youngster et al., ; Leuck et al., ; Menezes et al., ; Nidhi et al., ; Nieto et al., ; Sahin et al., ).…”