GENETIC DIVERSITY OF MEASLES VIRUS - RESURGENCE OF NEW GENOTYPE D8 IN São Paulo, BRAZIL

Oliveira, Maria Isabel de; Afonso, Ana Maria Sardinha; Adelaide, Crsitina Figueiredo de; Lemos, Xênia Rawena Medeiros Romeu; Almeida, Jalusy; Yu, Ana Lúcia Frugis; Curti, Suely Pires

doi:10.1590/s0036-46652014000400018

Cited by 5 publications

(6 citation statements)

References 3 publications

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“…In Brazil, all cases of fever and rash meeting clinical case definitions for measles, defined as fever and maculopapular rash accompanied by ≥1 of the 3 following symptoms: cough, coryza, or conjunctivitis, in any individual, regardless of age or vaccination status or rubella, defined as fever and maculopapular rash accompanied by retroauricular, occipital, or cervical lymphadenopathy in any individual, regardless of age and vaccination status, should be notified and confirmed or discarded by laboratory testing 11 , 12 . In accordance with the established procedures of the regional measles/rubella laboratory network, composed of Public Health laboratories throughout the State of Sao Paulo, laboratory evaluation of suspected cases and contacts include serologic testing, viral culture, detection of viral RNA by Real Time PCR and viral genotyping 1 , 3 , 7 . For this reason, the present patient was at first misdiagnosed with rubella and only later the correct diagnosis was made.…”

Section: Discussionmentioning

confidence: 99%

“…Viral RNA extraction was performed from urine samples and infected culture cells were processed using PureLink Viral RNA/DNA Mini Kit (Invitrogen, USA) according to the manufacturer’s recommendations. Initially, the Real Time PCR (RT-qPCR) was performed using Measles virus (MV) and Rubella virus (RV) primers, as previously described 1 , 7 . The urine sample and infected culture cells were negative for MV and RV.…”

Section: Case Reportmentioning

confidence: 99%

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Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

Oliveira

Namiyama

Cabral

et al. 2018

Rev. Inst. Med. trop. S. Paulo

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A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage.

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Section: Discussionmentioning

confidence: 99%

Section: Case Reportmentioning

confidence: 99%

Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

Oliveira

Namiyama

Cabral

et al. 2018

Rev. Inst. Med. trop. S. Paulo

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“…In conclusion, the measles outbreak in Ceará was probably imported directly from Europe or from there through the bordering state of Pernambuco ( 4 , 5 , 9 ). Cases were concentrated in Fortaleza and the northwest region of the state.…”

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confidence: 95%

Measles Reemergence in Ceará, Northeast Brazil, 15 Years after Elimination

Leite¹,

Barreto²,

Sousa³

2015

Emerg. Infect. Dis.

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“…Previous studies in Brazil have primarily concentrated on genotyping a small fragment of 450bp of the measles nucleoprotein (9,10). Although, these studies have undoubtedly contributed to the molecular epidemiology of measles, sequencing larger genome fragments or better yet, complete viral genomes, is pivotal for a better understanding of the epidemic dynamics of any emerging or remerging viral diseases (11,12).…”

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confidence: 99%

Complete genome of a Measles virus from Roraima state, Brazil

Meneses

Nascimento

Souza

et al. 2018

Preprint

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SUMMARYMeasles is a human infectious disease of global concern caused by the measles virus. In this study, we report the complete genome sequencing of one measles isolate, genotype D8, obtained in Boa Vista city, the capital of the Roraima State, Brazil, directly from the urine sample. Phylogenetics reconstruction grouped the genome described in this study with samples from Australia, Italy, United Kingdom, and the USA. To our knowledge, this is the first complete genome of a wild-type measles virus from Latin America. Therefore, the present data strengthens the current knowledge about the molecular epidemiology of measles worldwide.SponsorshipsCNPq / CAPES / MS-DECIT / Fiocruz

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GENETIC DIVERSITY OF MEASLES VIRUS - RESURGENCE OF NEW GENOTYPE D8 IN São Paulo, BRAZIL

Cited by 5 publications

References 3 publications

Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

Measles Reemergence in Ceará, Northeast Brazil, 15 Years after Elimination

Complete genome of a Measles virus from Roraima state, Brazil

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