2013
DOI: 10.1590/s0036-46652013000600004
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APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL

Abstract: SUMMARYThis study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emílio Ribas (IIER), a reference center for infectious diseases in São Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the traditional methods demonstrated the following percentages of positivity: 62.8% (49/78) for the Montenegro skin test,… Show more

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Cited by 24 publications
(19 citation statements)
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“…21 The side effects include liver failure, pancreatitis, constitutional symptoms, and abnormal electrocardiographic findings (T wave inversion; QTc interval prolonged), which can affect 10-30% of individuals treated. [21][22][23][24] These effects limit the use of antimonials in patients with previous comorbidities and advanced age. Amphotericin B deoxycholate, liposomal amphotericin, azole drugs, miltefosine, and pentamidine are recommended as a second-line therapeutic option and in cases of therapeutic failure with antimonials, according to the Pan American Health Organization guidelines.…”
Section: Discussionmentioning
confidence: 99%
“…21 The side effects include liver failure, pancreatitis, constitutional symptoms, and abnormal electrocardiographic findings (T wave inversion; QTc interval prolonged), which can affect 10-30% of individuals treated. [21][22][23][24] These effects limit the use of antimonials in patients with previous comorbidities and advanced age. Amphotericin B deoxycholate, liposomal amphotericin, azole drugs, miltefosine, and pentamidine are recommended as a second-line therapeutic option and in cases of therapeutic failure with antimonials, according to the Pan American Health Organization guidelines.…”
Section: Discussionmentioning
confidence: 99%
“…The parasite's kDNA and circular DNA were good targets for molecular diagnostic studies and identification of the species due to having thousands of variable and conserved copies. On the other hand, this region has many taxonomic applications due to its high number of copies and repetitive sequences in the parasite genome (20). Similar to some studies, the present study used kDNA (present in a unique organelle called kinetoplast) that is both specific and highly sensitive for diagnosis.…”
Section: Discussionmentioning
confidence: 93%
“…The later L5.8SR/ LITSV targeting ribosomal internal transcribed spacer 2 separating the genes coding for 5.8S rRNA and large subunit rRNA gene. [17,18], genomic DNA, splice leader mini-exon [19], telomeric repeats [20], rRNA gene [21] and gp63 [22].…”
Section: Methodsmentioning
confidence: 99%