MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS

Abstract

SUMMARY Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for SAB of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans.

Dissemination of macrolides, fusidic acid and mupirocin resistance among Staphylococcus aureus clinical isolates

“…Meanwhile, ST965 was found to be different in seven loci from ST338 (19-23-15-48-19-20-15), but they were assigned to one cluster. These findings suggest adaptation to varying environmental conditions occurs through microevolutionary changes within a single isolate [ 58 , 59 ]. Notably, the 23 isolates with the similar resistance phenotype and genotype belonged to various STs and clusters, revealing the genetic relatedness among these isolates was weak and there was a high genetic heterogeneity.…”

COMPARISON BETWEEN FOUR USUAL METHODS OF IDENTIFICATION OF Candida SPECIES

“…Among the various molecular techniques, the best known are: multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), duplex PCR, restriction fragment length polymorphisms (RFLP), random amplified polymorphic DNA (RAPD), length heterogeneity polymerase chain reaction (LH-PCR), and microsatellites 4 , 15 . The molecular techniques are accurate, sensitive, specific and relatively simple, and have the potential to greatly decrease the time of…”

Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present

“…RAPD afforded diverse profiles for both primers M2 and P4. Using CDC3 and HIS3 markers, microsatellite endorsed the observation of six and seven unlike alleles, respectively [244].…”