Bait formulations of molluscicides and their effects on biochemical changes in the ovotestis of snail Lymnaea acuminata (Mollusca; Gastropoda:Lymnaeidae)

Abstract

SUMMARYThe effect of sub-lethal feeding of bait formulations containing molluscicidal component of Ferula asafoetida (ferulic acid, umbelliferone), Syzygium aromaticum (eugenol) and Carum carvi (limonene) on biochemical changes in the ovotestis of snail Lymnaea acuminata were studied. Bait formulations feeding to L. acuminata were studied in clear glass aquaria having diameter of 30 cm. Baits were prepared from different binary combinations of attractant amino acid (valine, aspartic acid, lysine and alanine 10 mM) in 100 mL of 2% agar solution + sub-lethal (20% and 60% of 24h LC 50 ) doses of different molluscicides (ferulic acid, umbelliferone, eugenol and limonene). These baits caused maximum significant reduction in free amino acid, protein, DNA, RNA levels i.e. 41.37, 23.56, 48.36 and 14.29% of control in the ovotestis of the snail, respectively. Discontinuation of feeding after treatment of 60% of 96h LC 50 of molluscicide containing bait for next 72h caused a significant recovery in free amino acid, protein, DNA and RNA levels in the ovotestis of L. acuminata.

In vitro PHYTOTHERAPY OF VECTOR SNAILS BY BINARY COMBINATIONS OF LARVICIDAL ACTIVE COMPONENTS IN EFFECTIVE CONTROL OF FASCIOLIASIS

“…Ferulic acid and umbelliferone extracted from Ferula asafoetida root latex are potent molluscicides against L. acuminata 11 12 13. The antioxidant, anticarcinogenic, antispasmodic, antihelminthic activity of F. asafoetida extract and ferulic acid were reported by various workers5 6 20.…”

Feeding of Bait to SnailLymnaea acuminataand Their Effect on Certain Enzyme in the Nervous Tissue

“…The inhibition in ALP and AChE activities may be due to the direct interference of these active molluscicidal with enzyme. Kumar et al, [ 27 ] reported that there was a depletion of amino acid and reduction of protein and nucleic acid level in the ovotestis of L. acuminata when these active molluscicides were fed to snails in bait formulations. Alkaline phosphatase plays a critical role in protein synthesis [ 28 ], shell formation [ 29 ], and other secretary activities [ 30 ] and its inhibition may result in the reduction of protein level [ 22 , 31 ] in gastropods.…”

Enzyme Activity in the Nervous Tissue of <i>Lymnaea Acuminata</i> Fed to Different Bait Formulations

“…The inhibition in ALP and AChE activity may be due to the direct interference of these active molluscicidal with enzyme. Kumar et al, [24] reported that there was a depletion of amino acid and reduction of protein and nucleic acid level in the ovotestis of L. acuminata when these active molluscicidel were fed to snails in bait formulations. Alkaline phosphatase plays a critical role in protein synthesis [25], shell formation [26] and other secretary activities [27] and its inhibition may result reduction in protein level [19,28] in gastropods.…”