In vitro PHYTOTHERAPY OF VECTOR SNAILS BY BINARY COMBINATIONS OF LARVICIDAL ACTIVE COMPONENTS IN EFFECTIVE CONTROL OF FASCIOLIASIS

Kumari, Sunita; Kumar, Pradeep; Singh, Dinesh

doi:10.1590/s0036-46652013000500002

Cited by 9 publications

(10 citation statements)

References 22 publications

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“…recorded a mean mortality time of 8.11 min and 10.11 min, respectively [38]. A similar synergistic effect was reported with the binary combination of ferulic acid and azadirachtin against Fasciola larva in the snail Lymnaea acuminata, which was 64.28 times more effective than a single treatment with ferulic acid [43]. Rao and Singh [44] reported that the synergistic action in binary and tri-herbal combinations of A. indica and Cedrus deodara oil (1:7 ratio) and A. indica, piperonyl butoxide, and C. deodara at a ratio of 1:5:7 were found to be more toxic to L. acuminata than single treatment.…”

Section: Discussionsupporting

confidence: 61%

Development and Evaluation of Poly Herbal Molluscicidal Extracts for Control of Apple Snail (Pomacea maculata)

2017

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Golden Apple Snail (GAS) is the most destructive invasive rice pest in Southeast Asia. The cost of synthetic molluscicides, their toxicity to non-target organisms, and their persistence in the environment have propelled the research of plant-derived molluscicides. Most research efforts have focused on individual plant extracts for their molluscicidal potency against GAS and have not been proven to be entirely effective in rice field conditions. Selective combination of synergistically acting molluscicidal compounds from various plant extracts might be an effective alternative. In this direction, ethanolic extracts from six different plants (Neem, Tobacco, Nerium, Pongamia, Zinger, and Piper) were evaluated against Pomacea maculata Perry. Of the various combinations studied, a binary extract (1:1) of nerium and tobacco (LC 90 177.71 mg/L, 48 h), and two tri-herbal extract formulations (1:1:1) of (nerium + tobacco + piper) and (nerium + tobacco + neem) were found to be most effective, with LC 90 values of 180.35 mg/L and 191.52 mg/L, respectively, in laboratory conditions. The synergistic effect of combined herbal extracts resulted in significant reduction in LC 90 values of the individual extracts. The findings of this study demonstrate that the selective combinations of potent molluscicidal herbal extracts are effective for management of P. maculata under laboratory conditions.

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Section: Discussionsupporting

confidence: 61%

Development and Evaluation of Poly Herbal Molluscicidal Extracts for Control of Apple Snail (Pomacea maculata)

2017

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“…Cercariae shedding infected snails were identified according to morphological characteristics (large size, swollen foot, appeared yellowish in colour, slow locomotion, shedding cercaria appeared at the mouth of the snails, changed and shell morphology) as described by Largue et al [22] and Sunita et al [8]. The infected snails were allowed to acclimatize for 24 h in laboratory conditions.…”

Section: Methodsmentioning

confidence: 99%

“…The pH of the water was 7.1–7.5, and dissolved oxygen, free carbon dioxide, and bicarbonate alkalinity were 6.3–7.4 mgL −1 , 5.2–6.4 mgL −1 , and 103–105 mgL −1 , respectively. Snail L. acuminata and F. gigantica were identified by Zoological Survey of India (ZSI), Kolkata [8]. Different wavelengths of visible light were used to study the phototoxicity of pheophorbide.…”

Section: Methodsmentioning

confidence: 99%

“…Release of molluscicides in aquatic ecosystem for snail control also affects the other nontarget organisms. The Fasciola larval stages sporocyst, redia, and cercaria are in division phases of F. gigantica in the snail body [8]. Now a new approach is considered that if larvae of Fasciola will be destroyed in the snail body, the rate of infection can be reduced without killing the snails.…”

Section: Introductionmentioning

confidence: 99%

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Photomediated Larvicidal Activity of Pheophorbide a against Cercaria Larvae of Fasciola gigantica

Singh

2017

Scientifica

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Fasciolosis is a parasitic disease caused by Fasciola gigantica. The freshwater snail Lymnaea acuminata is the intermediate host of F. gigantica which cause endemic fasciolosis in the northern part of India. To investigate larvicidal activity of pure and laboratory extracted pheophorbide a (Pa) against cercaria larvae of F. gigantica, data were analyzed in different spectra of visible light, sunlight, and laboratory conditions. Photostimulation of chlorophyll derivative pheophorbide a (Pa) caused time and concentration dependent larvicidal activity against cercaria larvae of F. gigantica. Larvicidal activity of pure Pa under 650 nm and 400–650 nm (8 h LC50 0.006 mg/10 mL) was more pronounced than extracted Pa under same irradiations (650 nm LC50 0.12 mg/10 mL, 400–650 nm LC50 0.14 mg/10 mL). Lowest toxicity of pure (8 h LC50 0.14 mg/10 mL) and extracted Pa (8 h LC50 1.25 mg/10 mL) was noted under 400 nm. Pa was found to be toxic in laboratory conditions also. The results presented in this paper indicate that pheophorbide a possess potential larvicidal activity against Fasciola gigantica larvae in different wavelengths of visible light, sunlight, and laboratory conditions.

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“…The larval stages rediae and cercariae are the division phases of F. gigantica inside the snail body. [10][11][12] If these larvae will be destroyed by plant larvicides without killing the infected snails, the rate of Fasciola infection can be reduced. In the last three decades, we are using plant derived larvicides against host snails for effective control of fasciolosis.…”

Section: Introductionmentioning

confidence: 99%

Photoactivated chlorophyllin and acetylcholinesterase/ cytochrome oxidase activity in Fasciola gigantica cercaria larvae

Singh¹,

Singh²

2018

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Objective:The effect of chlorophyllin (CHL), CHL + freeze dried cow urine (FCU) and FCU on acetylcholinesterase (AChE) and cytochrome oxidase activity in the nervous tissue of the host snail Lymnaea acuminata, and parasitic cercaria larvae of Fasciola gigantica were studied. Methods: Whole cercaria larvae and snail nervous tissue was homogenized and supernatant was used as an enzyme source. Enzyme activity was measured in 10-mm path length cuvette using incubation mixtures and change in optical density was recorded. Results: Sublethal 4 h LC 50 40% and 80% of these formulations in sunlight and laboratory conditions caused no significant change in AChE activity in the nervous tissue of host snail. It was noted that sublethal in vivo 8 h exposure to 40% and 80% of 4 h LC 50 of single and binary combination of CHL+FCU, CHL and FCU significantly altered the AChE and cytochrome oxidase activity in the cercaria larvae. Binary combinations of CHL+FCU in sunlight were more effective in inhibiting both the enzymes. Conclusion: The present study reported that enzymes AChE and cytochrome oxidase were inhibited by chlorophyllin formulations and thus causing the larval death inside the snail body without affecting the snail itself which is an important bioindicator of aquatic ecosytem.

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In vitro PHYTOTHERAPY OF VECTOR SNAILS BY BINARY COMBINATIONS OF LARVICIDAL ACTIVE COMPONENTS IN EFFECTIVE CONTROL OF FASCIOLIASIS

Cited by 9 publications

References 22 publications

Development and Evaluation of Poly Herbal Molluscicidal Extracts for Control of Apple Snail (Pomacea maculata)

Development and Evaluation of Poly Herbal Molluscicidal Extracts for Control of Apple Snail (Pomacea maculata)

Photomediated Larvicidal Activity of Pheophorbide a against Cercaria Larvae of Fasciola gigantica

Photoactivated chlorophyllin and acetylcholinesterase/ cytochrome oxidase activity in Fasciola gigantica cercaria larvae

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