Candiduria in a public hospital of São Paulo (1999-2004): characteristics of the yeast isolates

Silva, Elza Helena da; Ruiz, Luciana da Silva; Matsumoto, Flavia E.; Auler, Marcos Ereno; Giudice, Mauro Cintra; Moreira, Débora Astoni; Szeszs, Walderez; Paula, Claudete Rodrigues

doi:10.1590/s0036-46652007000600003

Cited by 30 publications

(32 citation statements)

References 42 publications

(47 reference statements)

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“…This was also evident in a recent epidemiological study of 100 candiduria cases (between 1999 and 2004) in a paediatric hospital in Brazil. In this study, C. parapsilosis was only isolated on four occasions compared with 56 for C. albicans, 20 for C. tropicalis and 11 for C. glabrata (da Silva et al, 2007). Furthermore, despite the apparently reduced ability to adhere to silicone, C. parapsilosis strains exhibited a higher degree of intra-species heterogeneity compared with the two other species examined (Fig.…”

Section: Discussionmentioning

confidence: 67%

Silicone colonization by non-Candida albicans Candida species in the presence of urine

Silva

Negri

Henriques

et al. 2010

Journal of Medical Microbiology

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Urinary tract infections (UTIs) are the most common nosocomial infections and 80 % are related to the use of urinary catheters. Furthermore, Candida species are responsible for around 15 % of UTIs and an increasing involvement of non-Candida albicans Candida (NCAC) species (e.g. Candida glabrata, Candida tropicalis and Candida parapsilosis) has been recognized. Given the fact that silicone is frequently used in the manufacture of urinary catheters, the aim of this work was to compare both the adhesion and biofilm formation on silicone of different urinary clinical isolates of NCAC species (i.e. C. glabrata, C. tropicalis and C. parapsilosis) in the presence of urine. Several clinical isolates of NCAC species recovered from patients with UTIs, together with reference strains of each species, were examined. Adhesion and biofilm formation were performed in artificial urine and the biofilm biomass was assessed by crystal violet staining. Hydrophobicity and surface charge of cells was determined by measuring contact angles and zeta potential, respectively. The number of viable cells in biofilms was determined by enumeration of c.f.u. after appropriate culture. The biofilm structure was also examined by confocal laser scanning microscopy (CLSM). The results showed that all isolates adhered to silicone in a species-and strain-dependent manner with C. parapsilosis showing the lowest and C. glabrata the highest levels of adhesion. However, these differences in adhesion abilities cannot be correlated with surface properties since all strains examined were hydrophilic and exhibited a similar zeta potential. Despite a higher number of cultivable cells being recovered after 72 h of incubation, stronger biofilm formation was not observed and CLSM showed an absence of extracellular polymeric material for all isolates examined. In summary, this work demonstrated that all tested NCAC species were able to adhere to and survive on silicone in the presence of urine. Furthermore, C. glabrata strains presented higher colonization abilities than C. tropicalis and C. parapsilosis strains, a fact that might explain the larger role of C. glabrata colonization and disseminated infections in hospitalized and catheterized patients. INTRODUCTIONNosocomial infections constitute a serious public health problem, as they are a major cause of morbidity and mortality, and cause an increased time of hospitalization with associated enhanced healthcare costs (Tamura et al., 2003). Urinary tract infection (UTI) is one of the most common types of nosocomial infections (Schaberg et al., 1991;Tamura et al., 2003) and more than 80 % of nosocomial UTIs are related to the use of medical devices such as urinary catheters (Febré et al., 1999;Harris et al., 1999;Douglas, 2002;Chow et al., 2008). Furthermore, Candida species are responsible for around 80 % of fungal infections in the hospital environment and 10-15 % of UTIs are caused by these micro-organisms (Amer et al., 2004). Although the majority of infections are caused by Candida albicans (Richards et a...

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Section: Discussionmentioning

confidence: 67%

Silicone colonization by non-Candida albicans Candida species in the presence of urine

Silva

Negri

Henriques

et al. 2010

Journal of Medical Microbiology

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“…Em um estudo com 100 amostras de leveduras, isoladas de urina, provenientes de Hospital Público Infantil de São Paulo Brasil, no período de 1999-2004, as espécies mais freqüentes foram Candida albicans, seguida de Candida tropicalis, Candida glabrata e Candida parapsilosis 14 .…”

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Resistência a antifúngicos de Candida tropicalis isoladas no Estado do Ceará

Menezes

Mendes

Cunha

2009

Rev. Soc. Bras. Med. Trop.

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“…Bu nedenle, Saps, lokalize invaziv hastalara (özellikle vajinal enfeksiyonlarda) kıyasla kan dolaşımı enfeksiyonlarının patogenezi için daha az önemli görünmektedir (2) . İlginç bir veri olarak, Brezilya, Sao Paulo'da kandidürili hastalardan izole edilen C. parapsilosis suşlarının (n=4) hepsi proteolitik aktivite sergilemiştir (19) . Mohan ve Ballal'ın (20) çalışmalarında da, kan kültürlerin-den izole edilen 5 C. parapsilosis izolatının tümünde proteinaz aktivitesi belirlenmiştir.…”

Section: Discussionunclassified

“…Ayrıca, bazı araştırmacılar sistemik ve yüzeysel izolatları karşılaştırırken yalnızca kan dolaşımı izolatlarında fosfolipaz aktivitesini tanımlarken (17) , diğerleri yüzey-sel C. parapsilosis izolatlarında sistemik izolatlardan çok daha yüksek aktiviteler açıklamakta-dır (26) . Brezilya'nın Sao Paulo kentinde kandidüri'ye neden olan dört C. parapsilosis suşundan yalnızca bir tanesi fosfolipaz aktivitesi sergilemiştir (19) . Dolayısıyla bu aktivite bulguları çelişkilidir.…”

Section: Discussionunclassified

Comparison of secretory acid proteinase, phospholipase activity, and slime production in Candida parapsilosis complex strains isolated from blood cultures and other clinical samples

Güler¹,

Tosun²

2017

TMCD

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Candiduria in a public hospital of São Paulo (1999-2004): characteristics of the yeast isolates

Cited by 30 publications

References 42 publications

Silicone colonization by non-Candida albicans Candida species in the presence of urine

Silicone colonization by non-Candida albicans Candida species in the presence of urine

Resistência a antifúngicos de Candida tropicalis isoladas no Estado do Ceará

Comparison of secretory acid proteinase, phospholipase activity, and slime production in Candida parapsilosis complex strains isolated from blood cultures and other clinical samples

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