SUMMARYThe study involved 100 yeast isolates, obtained from urine samples provided by a Public Pediatric Hospital of São Paulo, Brazil, from 1999 to 2004. The most frequent species was Candida albicans, followed by C. tropicalis, C. glabrata and C. parapsilosis. In regard to virulence, 97% of the isolates showed index 3 for proteinase and 63% index 2 for phospholipase. The most frequent killer biotypes were 511 and 888.

Aims: Vulvovaginal candidiasis is one of the most common complaints in the medical clinic. In recent years, due to the increasing frequency of non-albicans species, the number of cases of therapeutic failure has increased considerably, generating the need for research to learn the profile of yeasts isolated in vulvovaginal candidiasis. The aims of this study were to collect samples of vaginal secretion, verify the presence of yeast, identify the species of yeast, and verify their in vitro susceptibility profile against six antifungal agents -amphotericin B, nystatin, fluconazole, itraconazole, ketoconazole and voriconazole. Materials and Methods: Vaginal secretion was collected from 130 patients with symptoms characteristic of vulvovaginal candidiasis (VVC). For amphotericin B, fluconazole, itraconazole, ketoconazole and voriconazole, the in-vitro tests were carried out using the commercial Etest susceptibility testing kit; for nystatin the disk diffusion method was used. Results: The yeasts isolated were: Candida albicans (90%), C. glabrata (5%), C. parapsilosis (3%) and C. tropicalis (2%). By the CLSI method, all the isolates were susceptible to fluconazole, ketoconazole, nystatin and voriconazole. Tests showed that 98.8% of the isolates were susceptible to amphotericin B and 97.6% to itraconazole. Conclusion: Although a high number of resistant strains were not found, these studies may help guide physicians to the most convenient therapeutic orientation, conducting treatment specific to the identified yeast species.