An evaluation of manual and mechanical methods to identify Candida spp. from human and animal sources

Abstract

To compare two yeast identification methods, i. e, the manual and the VITEK mechanical methods, 62 clinical samples from hemocultures and animal sources were analyzed. After identification as Candida yeasts by the VITEK method, the strains were recharacterized using manual assimilation methods and sugar fermentation tests. Our findings reveal 58% concurrent identification between the two methods for animal strains, and 51% for human hemoculture strains.

Oral rinse as a potential method to culture Candida isolate from AIDS patients

“…This is the reason for clinicians to identify correctly the microorganism causing the disease. [8][9][10] Eighteen patients recently admitted to the Intermediate Care Unit for Infectious Diseases were included in the study, however 4 of them must be excluded because 2 were barely conscious while the other 2 were not able to sit up. All 14, 12 males and 2 females, were diagnosed with stadium 4 AIDS, indicating CD4 + counts below 200/mm 3 .…”

“…The rest of the isolate can also be grown on to standard growth medium for candida species. 3,6,10 However, the gold method does not cover taking isolates from patients presenting less obvious form of candidiasis, such as the erythematous type. Patients with risk of contracting candidiasis, such as hospitalized patients and especially HIV positive ones, should receive early management for this debilitating opportunistic infection.…”

“…Phosphat buffer saline ensures the cells obtained are well preserved during transfer time to the laboratory. 8,10 By telling the patient to rinse his mouth, we also gather information about the patient's motoric and sensoric reflex. This will also be a consideration for clinician to prescribe topical antifungal and antiseptic in the form of rinse or gargle.…”

Molecular Diagnosis Compared to Conventional Diagnosis for Rapid Detection of Resistant Strains of Candida Spp.

“…Currently, mycoses are important opportunistic infections in immunosuppressed individuals, and their diagnoses and treatments are decisive, since, in some cases, these can lead to a possible death (Silveira, 2017). Several systems for yeast culture capable of identifying these pathogens have been developed and require a time between 4 to 72 hours for analysis of the results, being widely available and commercialized (Espinel-Ingroff et al, 1998;Silva;Candido, 2005;Oliveira et al, 2006). Although correct identification of clinically important yeast strains can be achieved with these systems, incomplete or incorrect identifications can occur when new and emerging yeast strains are tested (Pfaller et al, 1996;Morschhäuser, 2016).…”

“…Early detection of infection has a major impact on the clinical course of many infectious diseases (Back-Brito et al, 2009). Morphological features and biochemical tests useful to identify isolated fungi may take days or even weeks to grow in culture, and evaluation of test results requires knowledge of mycology (Oliveira et al, 2006;Back-Brito et al, 2009). In recent years, several DNA-based methods have been developed to improve the diagnosis of mycotic infections and the identification of pathogenic fungi (Luo & Mitchell, 2002;Morschhäuser, 2016).…”