Distribution of hepatitis C virus genotypes among blood donors from mid-west region of Brazil

Martins, Regina Maria Bringel; Teles, Sheila Araújo; Freitas, Nara Rubia de; Motta-Castro, Ana Rita Coimbra; Souto, Francisco José Dutra; Mussi, Aparecida Duarte Hg; Amorim, Regina Maria Santos de; Martins, Cláudio

doi:10.1590/s0036-46652006000100012

Cited by 32 publications

(27 citation statements)

References 13 publications

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“…The genotype of the other nine samples could not be determined due to insufficient material. These data were similar to those previously found in Brazil, where genotype 1 is predominant (1,4,26,31,36). These data are in contrast to the genotype distribution observed by Busek et al (8) in a study conducted among ten patients from a haemodialysis centre, where there was a higher prevalence of genotype 3 followed by genotypes 1 and 2.…”

Section: Discussionsupporting

confidence: 90%

Viral Hepatitis C in a leading Brazilian hospital: epidemiological factors and genotyping

Bezerra¹,

Lima²,

Vilar³

et al. 2007

Braz. J. Microbiol.

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The hepatitis C virus (HCV) is classified into six different genotypes and their distribution is different throughout the world. Epidemiologic studies are important to determine several characteristics of the virus, as well as the disease. This study analysed the prevalence of HCV and its genotypes among patients from a leading hospital in Ceará, which is located in Northeast Brazil. A total of 119 anti-HCV-seropositive patients, each having previously completed a questionnaire about risk behaviours related to HCV infection were tested for HCV infection using a qualitative HCV polymerase chain reaction (PCR) assay and genotyping by restriction fragment length polymorphism (RFLP). The detection was based on amplifying of the non-coding 5' region. Of the 119 patients, 95 showed positive results in the qualitative HCV test. History of surgery was the most reported risk factor, followed by the use of drugs, having tattoos, undergoing haemodialysis and occupational exposure. Genotype 1 was the most prevalent (46.9%), followed by genotype 3 (34.4%) and 2 (8.3%). The genotype distribution was similar for all of the various risk behaviours.

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Section: Discussionsupporting

confidence: 90%

Viral Hepatitis C in a leading Brazilian hospital: epidemiological factors and genotyping

Bezerra¹,

Lima²,

Vilar³

et al. 2007

Braz. J. Microbiol.

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“…Similarly, another study conducted in male prisoners from the same penitentiary complex found genotype 1 in 80% of the samples (1a = 60%), followed by genotype 3a (13.3%) 28. In this sense, the HCV genotypes circulating in Goiás prisoners reflect the diversity of this virus in Goiás, Central Brazil 29,30 .…”

Section: Methodsmentioning

confidence: 99%

Epidemiology of the viral hepatitis B and C in female prisoners of Metropolitan Regional Prison Complex in the State of Goiás, Central Brazil

Barros

Pessoni

Teles

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:Little information regarding hepatitis B virus (HBV) and hepatitis C virus (HCV) infections among Brazilian female prisoners exists. This study investigated the prevalence and risk factors associated with HBV and HCV infections and identified viral genotypes among female prisoners in Goiás, Central Brazil. Methods: Women incarcerated in the largest prison in the State of Goiás were invited to participate in the study. All female prisoners were interviewed and tested for the detection of hepatitis B surface antigen (HBsAg), antibodies against HBsAg (anti-HBs), against hepatitis B core antigen (anti-HBc), and antibody against HCV (anti-HCV) by ELISA. HBsAg and anti-HCV positive samples were tested for HBV DNA and HCV RNA and genotyped, respectively. Results: Participants (n=148; 98.6%) completed the study with an overall HBV prevalence of 18.9%. Age >30 years, a low education level, sex with a sexually transmitted diseases carrier, and a male sexual partner serving in the same penitentiary were associated with HBV infections. Only 24% of the women were anti-HBs positive suggesting previous HBV vaccination. Nine female prisoners (6.1%) were anti-HCV positive. Age >40 years, injecting drug use and length of incarceration were statistically associated with anti-HCV antibodies. Five samples were HCV RNA positive and classified as genotypes 1 (subtypes 1a; n=3 and 1b; n=1) and 3 (subtype 3a; n=1). The HBsAg-reactive sample was HBV DNA positive and genotype A. Conclusions: These findings highlight the necessity of public policies to control hepatitis B and C infections and emphasize the importance of hepatitis B vaccination in prison environments.

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“…In Brazil, several studies have been conducted to determine the distribution of HCV genotypes among different groups of individuals. Most of these studies have shown a predominance of genotype 1 (Krug et al 1996, Oliveira et al 1999, Paraná et al 2000, Busek & Oliveira 2002, Codes et al 2003, Vogler et al 2004, Martins et al 2006, Silva et al 2006. The aim of the present study was to determine with precision the frequency of each HCV genotype in the South of Brazil, by studying a large cohort of patients from Rio Grande do Sul (RS) and Santa Catarina (SC), the southernmost states of the country.…”

mentioning

confidence: 93%

High proportion of hepatitis C virus genotypes 1 and 3 in a large cohort of patients from Southern Brazil

Silva

Costi

Krug³

et al. 2007

Mem. Inst. Oswaldo Cruz

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Hepatitis C virus (HCV) isolates have been divided into six genotypes (1 to 6). The duration of hepatitis C standard treatment is 48 weeks for patients infected with HCV genotype 1 vs 24 weeks for those infected with genotypes 2 and 3. A total of 1544 HCV isolates from chronic patients living in the southern Brazilian states of Rio Grande do Sul (RS, n = 627) and Santa Catarina (SC, n = 917) were genotyped by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products. In RS, 338 (53.9%; 34 (5.4%; 255 (40.7%;.6%) samples were from genotypes 1, 2, and 3, respectively. In SC, 468 (51%; 26 (2.9%; 423 (46.1%; samples were from genotypes 1, 2, and 3, respectively. Genotyping results were confirmed by direct nucleotide sequencing of PCR products derived from 68 samples, without any discrepancy between PCR-RFLP and nucleotide sequencing methods. In conclusion, almost half of the hepatitis C patients from South of Brazil are infected by genotypes 2 and 3 and, these results have important consequential therapeutic implications as they can be treated for only 24 weeks, not 48.Key words: hepatitis C virus genotypes -polymerase chain reaction -restriction fragment lengh polymorphismnucleotide sequencing -Rio Grande do Sul -Santa CatarinaChronic hepatitis C, caused by infection with hepatitis C virus (HCV), is a global health problem, with more than 170 million infected individuals worldwide (WHO 1999). In 15 -20% of acute HCV infections, the patients recover spontaneously. However, in a large majority of cases, the disease runs a chronic course, clinically silent. Persistent infection may lead to severe liver diseases, such as cirrhosis and hepatocellular carcinoma, which require intensive treatment regimens and, in some cases, liver transplantation, and long-term care.HCV is primarily transmitted by the parenteral route. Sexual and perinatal transmissions have also been demonstrated, although less frequent (Poupon 2005). Since there is no available vaccine against hepatitis C, the only means to lower the disease burden are prevention of new infections and treatment of chronic carriers.HCV has a large genetic heterogeneity. Virus isolates have been classified into six genotypes (1 to 6), based on nucleotide sequence divergence of 30 -35% (Simmonds 2004). Genotypes 1, 2, and 3 circulate around the world, while genotypes 4, 5, and 6 are restricted to determined geographical areas (McOmish et al. 1994). In Brazil, genotypes 1, 2, and 3 predominate, although genotypes 4 and 5 have also been found (Campiotto et al. 2005).HCV genotype determination is necessary to define the optimal duration of therapy and the likelihood of response. According to the European Association for the Study of the Liver (EASL 1999) International Consensus Panel and National Institutes of Health (NIH 2002) Consensus Panel on management of hepatitis C, standard therapy for chronic HCV infection is based on combination of pegylated interferon (PEG IFN) alfa-2a or alfa-2b with ribavirin (RBV) during 48 weeks for patie...

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Distribution of hepatitis C virus genotypes among blood donors from mid-west region of Brazil

Cited by 32 publications

References 13 publications

Viral Hepatitis C in a leading Brazilian hospital: epidemiological factors and genotyping

Viral Hepatitis C in a leading Brazilian hospital: epidemiological factors and genotyping

Epidemiology of the viral hepatitis B and C in female prisoners of Metropolitan Regional Prison Complex in the State of Goiás, Central Brazil

High proportion of hepatitis C virus genotypes 1 and 3 in a large cohort of patients from Southern Brazil

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