Serotyping and evaluation of the virulence in mice of Streptococcus suis strains isolated from diseased pigs

Costa, Alfredo; Lobato, Francisco Carlos Faria; Abreu, V.L.V.; Assis, Ronnie Antunes de; Reis, Ronaldo; Uzal, Francisco A.

doi:10.1590/s0036-46652005000200012

Cited by 25 publications

(21 citation statements)

References 8 publications

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“…A few reports on serotype 2 strains recovered from diseased pigs in Brazil have been published (Costa et al , 2005; Doto et al , 2016; Rocha et al , 2012), though no human case has yet been reported. Human cases have been reported in Argentina (Lopreto et al , 2005; Callejo et al ., 2014; Nagel et al , 2008) and, only once, in Chile (Alarcon et al , 2013), but strains were not further characterized.…”

Section: Discussionmentioning

confidence: 99%

Streptococcus suis serotype 2 strains isolated in Argentina (South America) are different from those recovered in North America and present a higher risk for humans

Callejo

Zheng

et al. 2016

JMM Case Reports

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Introduction:Streptococcus suis serotype 2 is an important swine pathogen and emerging zoonotic agent causing meningitis and septicemia/septic shock. Strains are usually virulent (Eurasia) or of intermediate/low virulence (North America). Very few data regarding human and swine isolates from South America are available.Case presentation:Seventeen new human S. suis cases in Argentina (16 serotype 2 strains and a serotype 5 strain) are reported. Alongside, 14 isolates from pigs are analyzed: 12 from systemic disease, one from lungs and one from tonsils of a healthy animal. All human serotype 2 strains and most swine isolates are sequence type (ST) 1, as determined by multilocus sequence typing and present a mrp+/epf+/sly+ genotype typical of virulent Eurasian ST1 strains. The remaining two strains (recovered from swine lungs and tonsils) are ST28 and possess a mrp+/epf−/sly− genotype typical of low virulence North American strains. Representative human ST1 strains as well as one swine ST28 strain were analyzed by whole-genome sequencing and compared with genomes from GenBank. ST1 strains clustered together with three strains from Vietnam and this cluster is close to another one composed of 11 strains from the United Kingdom.Conclusion: Close contact with pigs/pork products, a good surveillance system, and the presence of potentially virulent Eurasian-like serotype 2 strains in Argentina may be an important factor contributing to the higher number of human cases observed. In fact, Argentina is now fifth among Western countries regarding the number of reported human cases after the Netherlands, France, the UK and Poland.

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Section: Discussionmentioning

confidence: 99%

Streptococcus suis serotype 2 strains isolated in Argentina (South America) are different from those recovered in North America and present a higher risk for humans

Callejo

Zheng

et al. 2016

JMM Case Reports

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“…Serotyping is an important step in the routine diagnostic procedure for S. suis infections. Different procedures have been described, but most laboratories use the coagglutination technique (Higgins & Gottschalk, 2001; Costa et al , 2005). Although the incidence of nontypeable isolates is in general low, their isolation is reported in the literature (Higgins & Gottschalk, 2000; Wei et al , 2009).…”

Section: Discussionmentioning

confidence: 99%

Cell surface characteristics of nontypeable isolates of Streptococcus suis

Bonifait

Gottschalk

Grenier

2010

FEMS Microbiology Letters

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Streptococcus suis is a worldwide cause of various swine infections and is also an important agent of zoonosis. Strains of S. suis are classified according to their serotype, and currently, 35 serotypes are recognized. The aim of this study was to characterize nontypeable isolates of S. suis with regard to their cell surface properties and compare them with serotype 2 strains, the most frequently associated with infections. The seven nontypeable strains of S. suis isolated from infected animals demonstrated a stronger capacity to adhere to a fibronectin-coated polystyrene surface than the serotype 2 isolates. Three nontypeable strains were also tested for their ability to adhere to endothelial cells and were found to attach in higher amounts compared with the serotype 2 isolates. Electron microscopy analysis revealed the absence of a capsule in the seven nontypeable isolates, which correlated with a much higher cell surface hydrophobicity than that of serotype 2 isolates. All nontypeable isolates of S. suis also showed the capacity to form a biofilm while serotype 2 isolates were unable to do so. In conclusion, the nontypeable isolates of S. suis examined in this study possess surface properties different from those of serotype 2 isolates.

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“…In particular the liver and lungs showed very similar infection patterns. It is generally accepted that the mouse is a good model for S. suis infection [1,29], although there have been some studies questioning the mouse model [31]. We believe that this mouse model can be used to examine the effect of a treatment with a potential anti-adhesion reagent.…”

Section: Discussionmentioning

confidence: 99%

“…Streptococcus suis is a major swine pathogen worldwide and is associated with meningitis, arthritis, endocarditis, septicemia, pneumonia and sudden death especially in young pigs [1]. S. suis is considered a zoonotic agent associated with human infections and has especially been described as the cause of meningitis in persons with occupational exposure to pigs [2,3].…”

Section: Introductionmentioning

confidence: 99%

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Use of Tetravalent Galabiose for Inhibition of Streptococcus Suis Serotype 2 Infection in a Mouse Model

Pieters

Slotved

Mortensen

et al. 2013

Biology

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Streptococcus suis is an important swine pathogen associated with a variety of infections such as meningitis, arthritis and septicemia. The bacterium is zoonotic and has been found to cause meningitis especially in humans occupationally exposed to infected pigs. Since adhesion is a prerequisite for colonization and subsequent infection, anti-adhesion treatment seems a natural alternative to traditional treatment with antibiotics. In order to optimize the inhibitory potency a multivalency approach was taken in the inhibitor design. A synthetic tetravalent galabiose compound was chosen which had previously shown promising anti-adhesion effects with S. suis in vitro. The aim of this study was to evaluate the in vivo effects of the compound using an infection peritonitis mouse model. As such S. suis serotype 2 infection and treatment were tested in vivo and the effects were compared to the effect of treatment with penicillin.

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Serotyping and evaluation of the virulence in mice of Streptococcus suis strains isolated from diseased pigs

Cited by 25 publications

References 8 publications

Streptococcus suis serotype 2 strains isolated in Argentina (South America) are different from those recovered in North America and present a higher risk for humans

Streptococcus suis serotype 2 strains isolated in Argentina (South America) are different from those recovered in North America and present a higher risk for humans

Cell surface characteristics of nontypeable isolates of Streptococcus suis

Use of Tetravalent Galabiose for Inhibition of Streptococcus Suis Serotype 2 Infection in a Mouse Model

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