Comparison between immunomagnetic separation, coupled with immunofluorescence, and the techniques of Faust et al. and of Lutz for the diagnosis of Giardia lamblia cysts in human feces

Abstract

SUMMARYIn the present study, the performance of Immunomagnetic Separation technique, coupled with Immunofluorescence (IMS-IFA), was compared with the FAUST et al. and Lutz parasitological techniques for the detection of Giardia lamblia cysts in human feces.One hundred and twenty-seven samples were evaluated by the three techniques at the same time showing a rate of cyst detection of 27.5% by IMS-IFA and 15. The use of this methodology as a routine procedure enables the processing of many samples simultaneously, in order to increase recovery rate of G. lamblia cysts and reduce the time of sample storage.

Evaluation of α-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia

“…Advanced technologies have been developed to detect G. lamblia, which include labeling by fluorescent in situ hybridization and quantum dots-labeled IFA [21], as well as real-time PCR [22]. IFA coupled with immunomagnetic separation is currently one of the main methods to detect Giardia [23]. In addition, PCR-RFLP had been used frequently in field investigations on the occurrence of Giardia among primary school children [24] or in wild animals [25].…”