Analysis of genetic relatedness between populations of Aedes aegypti from different geographic regions of São Paulo state, Brazil

Abstract

SUMMARYRAPD markers have been used for the analysis of genetic differentiation of Aedes aegypti, because they allow the study of genetic relationships among populations. The aim of this study was to identify populations in different geographic regions of the São Paulo State in order to understand the infestation pattern of A. aegypti. The dendrogram constructed with the combined data set of the RAPD patterns showed that the mosquitoes were segregated into two major clusters. Mosquitoes from the Western region of the São Paulo State constituted one cluster and the other was composed of mosquitoes from a laboratory strain and from a coastal city, where the largest Latin American port is located. These data are in agreement with the report on the infestation in the São Paulo State. The genetic proximity was greater between mosquitoes whose geographic origin was closer. However, mosquitoes from the coastal city were genetically closer to laboratory-reared mosquitoes than to field-collected mosquitoes from the São Paulo State. The origin of the infestation in this place remains unclear, but certainly it is related to mosquitoes of origins different from those that infested the West and North region of the State in the 80´s.

Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers

“…This agrees with the results of other genetic analyses of populations corresponding to several vector insects (mainly mosquitoes), where their reproducibility and polymorphism have also been shown (de Sousa et al, 1999;Gorrochotegui-Escalante et al, 2000;Fonseca et al, 2001;dos Santos et al, 2003;Julio et al, 2009). For instance, Ayres et al (2002), in study done on genetic diversity in 6 Brazilian Aedes albopictus populations, obtained just reproducibility and efficiency in 4 of the 10 primers tested.…”

Association of insecticide use and alteration on Aedes aegypti susceptibility status

“…Also, in Santos there is the biggest port in the country, and genetic characterization of Ae. aegypti showed that this population might have a distinct origin from other populations from SP (Santos 2003, Bracco et al 2007. In this sense the resistance observed in Ae.…”

Genetic Diversity in Dengue Mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon Region: Comparative Analysis with Isozymes and RAPD Loci

“…In this context, RAPD markers have shown genetic differentiation of Ae. aegypti populations all over the world [30,32] and in Brazil [12,31,33]. Gorrochotegui-Escalante et al (2000) [30] analyzed populations of Ae.…”

“…aegypti, Ayres et al (2003) [31] analyzed 47 RAPD polymorphic loci in 15 populations from five states, and detected a high level of genetic differentiation, suggesting population structuring. Santos et al (2003) [33] analyzed populations from different regions of São Paulo, using these markers, and observed that genetic similarity was higher among mosquitoes whose geographic origins were closer. Paduan et al (2006) [12], studying Ae.…”