A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)

Rocha, Sérgio M.; Suzuki, Lisandra Akemi; Silva, Andréa D.T. da; Arruda, Gisele Cristina; Rossi, Claudio

doi:10.1590/s0036-46652002000100011

Cited by 13 publications

(11 citation statements)

References 7 publications

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“…Since then, the LAT has been used to detect antibodies in a variety of parasitic diseases such as visceral leishmaniasis,[15] toxoplasmosis,[16] cystic echinococcosis,[17] and neurocysticercosis. [18]…”

Section: Discussionmentioning

confidence: 99%

A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

Biswas

Parija

2011

Trop Parasitol

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Background:Simple and rapid latex-based diagnostic tests have been used for detecting specific antigens or antibodies in several diseases.Aims:The aim of the present study was to standardize and evaluate the latex agglutination test (LAT) for the detection of Taenia solium metacestode antigen in the cerebrospinal fluid (CSF) and serum for the diagnosis of neurocysticercosis (NCC).Settings and Design:The study was conducted at Department of Microbiology, Jawaharlal Institute of Post graduate medical education and research after obtaining informed consent from the study subjects.Materials and Methods:In the present study, CSF and serum samples were collected from clinically suspected NCC, CT/MRI proven cases of NCC, non-cysticercal central nervous system infection control and from healthy control subjects. CSF was not collected from healthy controls. Polyclonal antisera raised in rabbits against porcine T. solium metacestode complete homogenate antigen, was used in the LAT to detect the antigen in the specimens.Statistical Analysis Used:The statistical analysis was carried out using Epi Info. The sensitivity, specificity, positive predictive value, and negative predictive value of the LAT were calculated.Results:The LAT exhibited sensitivity of 64.7% and specificity of 85.7% with CSF samples and sensitivity of 52.08% and specificity of 96% with serum samples.Conclusions:Results of the present study shows that the LAT can be employed as a moderately sensitive and specific test for the detection of T. solium metacestode antigen in the CSF and serum specimens for the diagnosis of NCC in poorly equipped laboratories.

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Section: Discussionmentioning

confidence: 99%

A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

Biswas

Parija

2011

Trop Parasitol

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“…The beads, with a density of 1.05 g/cm 3 , were modified to bind with a carboxyl functional group (COOH − ) at their surfaces. The antibodies were coupled to carboxylcoated nanobeads via the standard EDC/NHS cross-linking protocol (Cao et al 2006;Rocha et al 2002). A shaker and several types of porous membrane filters with various aperture sizes were used to reduce the particle self-assembly and filter out particle clusters.…”

Section: Methodsmentioning

confidence: 99%

TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

Fan

Sheen

Chen

et al. 2015

Microfluid Nanofluid

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“…23 A sonicator and several types of porous membrane lters with different aperture sizes were used to reduce self-assembly of beads and lter bead clusters. Polystyrene beads with diameters of 300 nm (Merck™ XC030) and 500 nm (Merck™ XC050), which are observable sizes of particles in our system, were used.…”

Section: Methodsmentioning

confidence: 99%

Detection of orchid viruses by analyzing Brownian diffusion of nanobeads and virus–immunobead association

et al. 2015

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This paper describes a new sensing technique for detecting orchid viruses by measuring the Brownian diffusion of immunobeads in liquid samples. Both the capsid proteins and virus particles of Odontoglossum ringspot virus (ORSV) are detectable targets by using this technique. When the targets bind to immunobeads, the Brownian diffusion of the beads decreases. Thereafter, the kinetic model of the antibody-antigen interaction in a free liquid space can be established. This model is used to calculate the association constant rate, dissociation constant rate, and dissociation constant of ORSV capsid proteins and antibody-coated nanobeads. This paper presents the results of using 300 nm immunobeads to detect ORSV capsid proteins and particles in a phosphate-buffered saline solution, as well as the results of using 500 nm beads to detect ORSV particles in a plant sap solution. In addition, transmission electron microscopy images of the antibody-coated nanobeads reacting to low and high concentrations of the ORSV are shown.

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A rapid latex agglutination test for the detection of anti-cysticercus antibodies in cerebrospinal fluid (CSF)

Cited by 13 publications

References 7 publications

A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

A rapid slide agglutination test for the diagnosis of neurocysticercosis in the rural health set up

TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

Detection of orchid viruses by analyzing Brownian diffusion of nanobeads and virus–immunobead association

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